本研究結果顯示，增加之無機營養鹽將影響浮游生物群聚組成 變化，以超微型浮游植物最先受到促進生長，而後其他浮游植物與異 營性細菌接續增加。不同之擾動強度處理，其差異則為培養瓶中的海 水組成比例及其相對應之化學與生物組成。由營養鹽時序變化得知實 驗組中的浮游生物群聚皆對於無機營養鹽 (DIN：Nitrate+Nitrite；
Phosphate；Silicate)有明顯需求。結果建議無機營養鹽會影響浮游生
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表一、CR1015 航次參數相關分析(DIN：無機氮鹽；SiO4-：矽酸鹽；PO4-：磷酸鹽；TB：總計細菌豐度；Syn.：聚球藻；Pro.：原核綠 藻；Pico：真核超微浮游植物；Pico-P：超微浮游植物；Heter：異營性細菌；Chl_a：葉綠素 a，Cyano：藍綠藻；Proch：原核 綠藻；Chloro：綠藻；Pras：青綠藻；Dinof：渦鞭毛藻；Prymn：鈣板藻；Crypt：隱藻；Diatoms：矽藻；Chrys：黃金藻；**: p
< 0.01；*: p < 0.05；ns: 不顯著。)。
表二、CR1015 航次浮游藻類生物量比值時序變化表 (Cyano：藍綠藻；Proch：原核綠藻；Chloro：綠藻；Pras：青綠藻；Dinof：渦鞭
圖 1、南海海域地形彩繪圖（資料來源：海洋學門資料庫）。紅點處為採樣點。
SEATS Dongsha
圖 2-1、培養實驗步驟示意圖。
圖 3-2、培養實驗操作實景。
圖 4、各航次無機氮濃度之時序變化圖。
圖 5、各航次磷酸鹽濃度之時序變化圖。
圖 6、各航次矽酸鹽濃度之時序變化圖。
圖 7、各航次異營細菌豐度之時序變化圖。
圖 8、葉綠素 a 濃度之時序變化圖。
圖 9、CR1010 浮游植物群聚生物量之時序變化圖。
圖 10、CR1015 浮游植物群聚生物量之時序變化圖。
圖 11、CR1015 航次超微浮游植物豐度、異營性細菌豐度、葉綠素 a 濃度之時序 變化圖
附錄一、高壓液相呈析衝堤方程式。
時間 流速速率 A B C Conditons
Min ml/min % % %
0 1.0 100 0 0 Injection
4 1.0 0 100 0 Linear gradient
18 1.0 0 20 80 Linear gradient
21 1.0 0 100 0 Linear gradient
24 1.0 100 0 0 Linear gradient
29 1.0 100 0 0 Equilibration
(Wright et al, 1991)
附錄二、指紋色素一覽表。
Pigment Algal
Chlorophyll a All class except Prochlorophyceae (Proch) Chlorophyll b Cholorophyceae (Chloro), Prasinophyceae (Pras)
Chlorophyll c2 Bacillariophyceae (Bacilli), Prymnesiophyceae (Prymn), Chrysophytes (Chrys), Dinoflagellates (Dinof)
Chlorophyll c3 Prymn, Chrys Alloxanthin)--- Cryptophyceae 19'- butanoyloxyfucoxanthin Chry, Prymn
Diadinoxanthi Bacilli, Dinof, Prymn, Chrys Fucoxanthin Bacilli, Prymn, Chrys
19'- hexanoyloxyfucoxanthin Chloro, Pras Lutein Chloro, Peas
Peridin Dinof
Prasinoxanthin Pras
Zeaxanthin Cyanobacteria, Proch
附錄三、浮游植物名稱中英文對照表。
浮游藻類 俗名、常用名稱 名稱
Golden-brown algal line
Bacillariophyta(Bacilli) Diatoms 矽藻 Dinophyta (Dinof) Dinoflagellates 渦鞭毛藻 Chrysophyceae (Chrys) Chrysophytes 黃金藻 Prymnesiophyceae (Prymn) Coccolithophorids 鈣板藻 Cryptophyta (Crypt) Cryptomonads 隱藻
Green algal line
Cholorophyceae (Chloro) Green algae 綠藻 Prasinophyceae (Pras) Green flaglellates 青綠藻
Blue-green algal line
Cyanophyta (Cyano) Cyanobacterium 藍綠藻 Prochlorophyta (Proch) Prochlorophytes 原核綠藻
附錄四、指紋色素起始比值矩陣資料表(Initial Ratio Matrix)。
Class / Pigment Chc3 Chc2 Peri 19Butfu Fuco Hexa Pras diad allo Diat zea Chl_b Chl_a
Cyanobacteria 0 0 0 0 0 0 0 0 0 0 2.06 0 1
Prochloro 0 0 0 0 0 0 0 0 0 0 0.27 0.33 1
Chlorophytes 0 0 0 0 0 0 0 0.13 0 0 0.01 0.46 1
Prasinophytes 0 0 0 0 0 0 0.36 0.28 0 0 0 0 1
Dinophyta 0 0.53 1.06 0 0 0 0 0 0 0.04 0 0 1
Prymnes 0.06 0 0 0 0 1.71 0 0 0 0 0 0 1
Cryptophytes 0 0.13 0 0 0 0 0 0 0.29 0 0 0 1
Diatoms 0 0 0 0 0.75 0 0 0 0 0 0 0 1
Chrys 0.25 0.25 0 0.37 0.05 0 0 0 0 0.03 0 0 1
附錄五、指紋色素最終比值矩陣資料表(Final Ratio Matrix；F1)。
ChC3 Chc2 Peri 19Butfu Fucox hexa Pras diad allo Diat zea Chl_b Chl_a
Cyanobacteria 0 0 0 0 0 0 0 0 0 0 0.67 0 0.33
Prochloro 0 0 0 0 0 0 0 0 0 0 0.17 0.21 0.63
Chlorophytes 0 0 0 0 0 0 0 0.09 0 0 0.01 0.29 0.62
Prasinophytes 0 0 0 0 0 0 0.22 0.17 0 0 0 0 0.61
Dinophy 0 0.10 0.45 0 0 0 0 0 0 0.02 0 0 0.43
Prymnes 0.02 0 0 0 0 0.62 0 0 0 0 0 0 0.36
Cryptophytes 0 0.09 0 0 0 0 0 0 0.2 0 0 0 0.71
Diatoms 0 0 0 0 0.43 0 0 0 0 0 0 0 0.57
Chrysop 0.12 0.17 0 0.18 0.02 0 0 0 0 0.01 0 0 0.49