本研究利用流動式及批次式亞臨界水系統應用於檸檬桉葉內部防蚊成分香茅 醛之提取並與乙酸乙酯萃取法做比較,以流動式亞臨界水系統處理 2 g 檸檬桉葉後 所檢測到之最高訊號強度為 15 pA,代入檢量線中得到其濃度為 31.98 μg/mL,萃 取效率為 0.048%;而以批次式亞臨界水系統處理 20 g 檸檬桉葉後所檢測到之訊號 強度為 20 pA,帶入檢量線中得到其濃度為 41.77 μg/mL,萃取效率為 0.021%;以 乙酸乙酯 15mL 萃取 2 g 檸檬桉葉後所檢測到之訊號強度為 600 pA,代入檢量線中 得到其濃度為 1177 μg/mL,萃取效率為 0.88%。相比下,流動式之萃取效率高於 批次式,但兩者之萃取效率皆未高於有機溶劑萃取,甚至低了許多。以標準品進 行測試後推測其原因為香茅醛在高溫環境下會發生降解反應,因此造成其萃取效 率低落。
未來擬於動態式亞臨界水系統之萃取管前再加一段預熱管,將液體先於預熱 管中加熱至亞臨界狀態後,再將亞臨界水導入後端放有樹葉粉末的萃取管柱中,
期望將樹葉粉末暴露於高溫狀態的時間降低,以避免其降解,進而能達成較好的 萃取效率。未來研究之架構圖如 5-1 所示。
圖 5-1 未來擬使用之亞臨界水架構圖
以批次式亞臨界水系統處理廚餘部分,本研究嘗試以不同溫度進行單次開閥 實驗並比較其結果,發現 160°C 時能源利用效率最佳,但在實際應用上,單次開 閥所排去水分過少。因此以含水率 55%,重量為 65 g 之米飯進行定時多次開閥實 驗,實驗後含水率降至 10%以下,重量為 32 g,而實驗前後具有顯著含水率差異,
證明了多次開閥在實際應用上具有可行性。亦以 5 atm 為標準進行定壓開閥實驗,
將米飯進行定壓開閥實驗,亦成功將含水率由實驗前的 62%降至 10%以下。因此 目前初步實驗結果顯示以批次式亞臨界水系統處理廚餘可能具有可行性,但目前 亦有能源利用效率低落之問題。
未來擬以更多具有代表性之廚餘,例如纖維素類及蛋白質類,重複此實驗;
並必須增加能源利用之效率,才有進一步商品化成為處理社區廚餘系統之可能 性。
參考文獻
1. Amashukeli, X., Pelletier, C. C., Kirby, J. P., & Grunthaner, F. J. 2007. Subcritical water extraction of amino acids from Atacama Desert soils. Journal of Geophysical Research: Biogeosciences, 112(G4).
2. Arapitsas, P., & Turner, C. 2008. Pressurized solvent extraction and monolithic column-HPLC/DAD analysis of anthocyanins in red cabbage. Talanta, 74(5), 1218-1223.
3. Areeprasert, C., Ma, D., Prayoga, P., & Yoshikawa, K. 2016. A Review on Pilot-Scale Applications of Hydrothermal Treatment for Upgrading Waste Materials. International Journal of Environmental Science and Development, 7(6), 425.
4. Barnard, D. R., Bernier, U. R., Posey, K. H., & Xue, R. D. 2002. Repellency of IR3535, KBR3023, para-menthane-3, 8-diol, and deet to black salt marsh mosquitoes (Diptera: Culicidae) in the Everglades National Park. Journal of medical entomology, 39(6), 895-899.
5. Barnard, D. R., & Xue, R. D. 2004. Laboratory evaluation of mosquito repellents against Aedes albopictus, Culex nigripalpus, and Ochlerotatus triseriatus (Diptera:
Culicidae). Journal of medical entomology, 41(4), 726-730.
6. Batish, D. R., Singh, H. P., Kohli, R. K., & Kaur, S. 2008. Eucalyptus essential oil as a natural pesticide. Forest Ecology and Management, 256(12), 2166-2174.
7. Bossou, A. D., Ahoussi, E., Ruysbergh, E., Adams, A., Smagghe, G., De Kimpe, N., ... & Mangelinckx, S. 2015. Characterization of volatile compounds from three Cymbopogon species and Eucalyptus citriodora from Benin and their insecticidal activities against Tribolium castaneum. Industrial Crops and Products, 76, 306-317.
8. Carroll, S. P., & Loye, J. 2006. PMD, a registered botanical mosquito repellent with deet-like efficacy. Journal of the American Mosquito Control Association, 22(3), 507-514.
9. Drapeau, J., Rossano, M., Touraud, D., Obermayr, U., Geier, M., Rose, A., & Kunz, W. 2011. Green synthesis of para-Menthane-3, 8-diol from Eucalyptus citriodora:
Application for repellent products. Comptes Rendus Chimie, 14(7), 629-635.
10. Gbashi, S., Adebo, O. A., Piater, L., Madala, N. E., & Njobeh, P. B. 2017.
Subcritical water extraction of biological materials. Separation & Purification Reviews, 46(1), 21-34.
11. Hawthorne, S. B., Trembley, S., Moniot, C. L., Grabanski, C. B., & Miller, D. J.
2000. Static subcritical water extraction with simultaneous solid-phase extraction for determining polycyclic aromatic hydrocarbons on environmental solids. Journal of Chromatography A, 886(1), 237-244.
12. Husain, S. S., & Ali, M. 2013. Volatile oil constituents of the leaves of Eucalyptus citriodora and influence on clinically isolated pathogenic microorganisms. Journal of Scientific and Innovative Research, 2(5), 852-858.
13. Ibanez, E., Kubátová, A., Señoráns, F. J., Cavero, S., Reglero, G., & Hawthorne, S.
B. 2003. Subcritical water extraction of antioxidant compounds from rosemary plants. Journal of agricultural and food chemistry, 51(2), 375-382.
14. Jaenson, T. G., Garboui, S., & Pålsson, K. 2006. Repellency of oils of lemon eucalyptus, geranium, and lavender and the mosquito repellent MyggA natural to Ixodes ricinus (Acari: Ixodidae) in the laboratory and field. Journal of medical entomology, 43(4), 731-736.
15. Jimenez-Carmona, M. M., & Luque de Castro, M. D. 1999. Isolation of eucalyptus essential oil for GC-MS analysis by extraction with subcritical water. Chromatographia, 50(9), 578-582.
16. Khajenoori, M., Asl, A. H., Hormozi, F., Eikani, M. H., & Bidgoli, H. N. 2009.
Subcritical water extraction of essential oils from Zataria multiflora Boiss. Journal of food process engineering, 32(6), 804-816.
17. Lenardão, E. J., Botteselle, G. V., de Azambuja, F., Perin, G., & Jacob, R. G. 2007.
Citronellal as key compound in organic synthesis. Tetrahedron, 63(29), 6671-6712.
18. Liu, J., Chen, P., Yao, W., Wang, J., Wang, L., Deng, L., ... & Lei, J. 2015.
Subcritical water extraction of betulinic acid from birch bark. Industrial Crops and Products, 74, 557-565.
19. Lucia, A., Licastro, S., Zerba, E., & Masuh, H. 2008. Yield, chemical composition, and bioactivity of essential oils from 12 species of Eucalyptus on Aedes aegypti larvae. Entomologia experimentalis et applicata, 129(1), 107-114.
20. Manika, N., Mishra, P., Kumar, N., Chanotiya, C. S., & Bagchi, G. D. 2012. Effect
of season on yield and composition of the essential oil of Eucalyptus citriodora Hook. leaf grown in sub-tropical conditions of North India. Journal of Medicinal Plants Research, 6(14), 2875-2879.
21. Mann, T. S., Babu, G. K., Guleria, S., & Singh, B. 2013. Variation in the volatile oil composition of Eucalyptus citriodora produced by hydrodistillation and supercritical fluid extraction techniques. Natural product research, 27(7), 675-679.
22. McGowin, A. E., Adom, K. K., & Obubuafo, A. K. 2001. Screening of compost for PAHs and pesticides using static subcritical water extraction. Chemosphere, 45(6), 857-864.
23. Mpuhlu, Batsho. Synthesis of p-menthane-3, 8-diol. Diss. Nelson Mandela Metropolitan University, 2007.
24. Nathan, S. S. 2007. The use of Eucalyptus tereticornis Sm.(Myrtaceae) oil (leaf extract) as a natural larvicidal agent against the malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Bioresource technology, 98(9), 1856-1860.
25. Ozel, M. Z., Gogus, F., & Lewis, A. C. 2003. Subcritical water extraction of essential oils from Thymbra spicata. Food Chemistry, 82(3), 381-386.
26. Peker, H., Srinivasan, M. P., Smith, J. M., & McCoy, B. J. 1992. Caffeine extraction rates from coffee beans with supercritical carbon dioxide. AIChE Journal, 38(5), 761-770.
27. Plaza, M., & Turner, C. 2015. Pressurized hot water extraction of bioactives. TrAC Trends in Analytical Chemistry, 71, 39-54.
28. Pulavarty, A., Kukde, S., Shinde, V. M., & Sarangi, B. K. 2016. Morphological, physiological and biochemical adaptations of Eucalyptus citriodora seedlings under NaCl stress in hydroponic conditions. Acta physiologiae plantarum, 38(1), 20.
29. Ramezani, H., Singh, H. P., Batish, D. R., Kohli, R. K., & Dargan, J. S. 2002.
Fungicidal effect of volatile oils from Eucalyptus citriodora and its major constituent citronellal. New zealand plant protection, 327-330.
30. Saldana, M. D., & Valdivieso-Ramírez, C. S. 2015. Pressurized fluid systems:
Phytochemical production from biomass. The Journal of Supercritical Fluids, 96, 228-244.
31. Satoh, A., Utamura, H., Nakade, T., & Nishimura, H. 1995. Absolute configuration
of a new mosquito repellent,(+)-eucamalol and the repellent activity of its epimer. Bioscience, biotechnology, and biochemistry, 59(6), 1139-1141.
32. Siddique, Y. H., Mujtaba, S. F., Jyoti, S., & Naz, F. 2013. GC–MS analysis of Eucalyptus citriodora leaf extract and its role on the dietary supplementation in transgenic Drosophila model of Parkinson’s disease. Food and chemical toxicology, 55, 29-35.
33. Singh, H. P., Kaur, S., Negi, K., Kumari, S., Saini, V., Batish, D. R., & Kohli, R. K.
2012. Assessment of in vitro antioxidant activity of essential oil of Eucalyptus citriodora ( lemon-scented Eucalypt; Myrtaceae ) and its major constituents. LWT-Food Science and Technology, 48(2), 237-241.
34. Singh, H. P., Mittal, S., Kaur, S., Batish, D. R., & Kohli, R. K. 2009.
Characterization and antioxidant activity of essential oils from fresh and decaying leaves of Eucalyptus tereticornis. Journal of agricultural and food chemistry, 57
(15), 6962-6966.
35. Specos, M. M., Garcia, J. J., Tornesello, J., Marino, P., Della Vecchia, M., Tesoriero, M. D., & Hermida, L. G. 2010. Microencapsulated citronella oil for mosquito repellent finishing of cotton textiles. Transactions of the Royal Society of Tropical Medicine and Hygiene, 104(10), 653-658.
36. Su, Y. C., Ho, C. L., Wang, E. I., & Chang, S. T. 2006. Antifungal activities and chemical compositions of essential oils from leaves of four eucalypts. Taiwan J For Sci, 21(1), 49-61.
37. Sudakin, D. L., & Trevathan, W. R. 2003. DEET: a review and update of safety and risk in the general population. Journal of Toxicology: Clinical Toxicology, 41(6), 831-839.
38. Tian, Y., Liu, X., Zhou, Y., & Guo, Z. 2005. Extraction and determination of volatile constituents in leaves of Eucalyptus citriodora. Se pu= Chinese journal of chromatography, 23(6), 651.
39. Vargaftik, N. B., Volkov, B. N., & Voljak, L. D. 1983. International tables of the surface tension of water. Journal of Physical and Chemical Reference Data, 12(3), 817-820.
40. Wang, S. F., Wang, W. H., Chang, K., Chen, Y. H., Tseng, S. P., Yen, C. H., ... &
Chen, Y. M. A. 2016. Severe dengue fever outbreak in Taiwan. The American journal of tropical medicine and hygiene, 94(1), 193-197.
41. Yang, Y., Bowadt, S., Hawthorne, S. B., & Miller, D. J. 1995. Subcritical water extraction of polychlorinated biphenyls from soil and sediment. Analytical Chemistry, 67(24), 4571-4576.
42. Yang, Y., Bowadt, S., Hawthorne, S. B., & Miller, D. J. 1995. Subcritical water extraction of polychlorinated biphenyls from soil and sediment. Analytical Chemistry, 67(24), 4571-4576.
43. Yuasa, Y., Tsuruta, H., & Yuasa, Y. 2000. A Practical and Efficient Synthesis of p-Menthane-3, 8-diols. Organic Process Research & Development, 4(3), 159-161.
44. Zakaria, S. M., & Kamal, S. M. M. 2016. Subcritical water extraction of bioactive compounds from plants and algae: applications in pharmaceutical and food ingredients. Food Engineering Reviews, 8(1), 23-34.