KA 誘發 SD 雄性大鼠癲癇發作後餵食天麻與控制組相較，
海馬 CA3 形態較完整，細胞凋亡較少，神經細胞存活較多，因此推
論天麻有神經保護作用，至於機制需要進一步的研究。
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The neuroprotection of Gastrodia elata BL on epileptic seizures induced by kainic acid in Sprague-Dawley rats
英文摘要
Student: Wei-Tsung Hsu Major professor: Ching-Liang Hsieh
Institute of Chinese Medical Science, China Medical University
Gstrodia elata BL was used to treat wind disease by ancient chinese medical doctor,.in order to treat epilepsy, stroke and headache. In the past, we had researched and found that Gstrodia elata BL could delay seizure attack and decrease the severity of epileptic seizure which may due to its anti-oxidant, anti-inflammation and
anti-apoptosis. But we found that Gstrodia elata BL or its active compound used to treat epileptic seizure was given before epilepsy induced, and it was not compared to the clinic. This time, we use intrahippocampal kainate injection method with 0.5 ug/ul KA to induce epileptic seizure attack and after EMG and EEG recorded, rats were randomized to four groups: first group was given Gstrodia elata extract orally 1 g/kg/day, the second group was administered valproic acid 250 mg/kg/day orally, the third group was given PBS orally 1 ml/day, and the other group replaced kaiante with PBS to intrahippocampal injection. After four days drug admistered, rats were
sarcrifed and corobal brain section was done. We found that in HE stain, the
morphology of hippocampal CA3 pyramidal cell were more intact although neuronal shrinkage with pycnotic nucleus was found. TUNEL positive cells was significantly less in Gstrodia elata group than in control group.（Gstrodia elata group
p<0.001;valproic acid group p<0.05），In immunohistochemitry stain, NeuN positive cells were more in Gstrodia elata group than in control group but there was no
significantly different between Gstrodia elata group and control grouping FJB stain.
Even though, Gstrodia elata group can decrease cell death and preserve more neurons after elileptic seizure 4 days later, so its may have neuroprotection to kainate treated epileptic rats.
Keyword: Gstrodia elata BL, kainic acid, intrahippocampal injection, NeuN immunostaining, Tunel staining, FJB immunostaining