According to our results, BM MSCs derived from children with SAA had poor potential of proliferation and differentiation and these alterations may be important in the pathogenesis of the disease. Our findings should be useful in further understanding of the pathophysiology of SAA and consequently lead to the development of novel treatment modalities. Further studies are needed to elucidate the relationship between MSCs and SAA.
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Figure 1. MSCs of controls and SAA children shared a similar
spindle-shaped morphology (A, ×100 magnification) and a consistent immunophenotypic profile which was negative for CD45, CD14 and CD34, and positive for CD105, CD73, and CD44 (B). Black and dotted lines indicate cultured cells from controls and SAA children stained with antibodies, respectively. The respective isotype controls are shown as grey lines.
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Figure 2. Proliferative capacity. (A) Average PD of passage 4 to 6 of
control and SAA group. (B) Comparison of cumulative PD, shown as mean and 95% confidence interval. Each circle represents a subject studied.
Figure 3. Differentiation potential. Osteogenic differentiation was
demonstrated by ALP activity (A) and von Kossa stain (B) after 3-week induction. Adipogenic differentiation was demonstrated by Oil red O stain (C) after 2-week induction. Magnification of micrographs: ×100.
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Figure 4. Comparison of osteogenic and adipogenic potential by
quantification of ALP activity (A) and Oil red O (B) stain spectrophotometrically, respectively. Mean and 95% confidence interval are illustrated. Each circle represents a subject studied.
Figure 5. Comparison of differentiation potential by lineage-specific
gene expression, Cbfa1 for osteogenesis (A) and lipoproteinlipase for adipogenesis (B). Mean and 95% confidence interval are illustrated.
Each circle represents a subject studied.