本實驗的目的，是利用離心分配層析的技術，搭配有機-水相溶劑系統，來 分離、純化桑葉中的有效成分，包括 Chlorogenic acid (綠原酸)、Isoquercitrin、
Astragalin 、 Quercetin 3-(6-malonylglucoside) (Q3MG) 、 Kaempferol 3-(6-malonylglucoside) (K3MG)，並用 HPLC-MS 來確定其結構。在本實驗當中 使 用 了 三 成 分 溶 劑 系 統 MTBE/Acetone/H2O 與 四 成 分 溶 劑 系 統 MTBE/Acetone/H2O/EA，皆成功的分離純化桑葉樣品。
本實驗中用 CPC 來分離 sample 1 中的有效成分 Isoquercitrin、Astragalin，
純度可達到約 97%，而 sample 2 中的有效成分 Chlorogenic acid (綠原酸)、
Quercetin 3-(6-malonylglucoside) (Q3MG) 、 Kaempferol 3-(6-malonylglucoside) (K3MG)，純度約為 70%，是因為 sample 1 中的成分被萃取到 sample 2 中，故在 萃取 sample 1 時，有機相與水相的平衡時間要夠長，以避免 sample 1 殘留在水 相中，最後被萃取到 sample 2 當中，造成純化結果不理想。未來可嘗試不同的 萃取條件，來增加分離的效果，並希望能達到純度提高的效果。
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