1. 由杜鵑、扁柏、蘚凋落物之淋洗實驗得知,植物在凋落後之淋洗初期,其所 含高度苯環化之DOC 即大部分被沖淋出來,且其水色深、含較多小分子量、較 親水性的苯環胺基酸,較易被微生物分解;而植物凋落較久所淋洗出的DOC,
以分子量較大、較疏水性、較難被分解的腐植物質為主。在本研究中,淋洗液之 DOC 大部分(約占 75%)為腐植物質。凋落物在淋洗約 400 天時,淋洗液的水色 仍持續波動甚至變深,對照其淋出之DOC 量也呈現相同之趨勢,顯示在鴛鴦湖 湖區的氣候條件下,凋落物腐化分解作用緩慢。在扁柏為優勢植被的鴛鴦湖集水 區,林下的附生植物(如苔蘚類)受酸雨淋洗所產生更酸的淋洗液,可能是使此地 區土壤及湖水偏酸性的主要原因。
2. 由腐植物質萃取實驗及螢光光譜圖(EEMs)得知,湖心表水之 DOC 以外源 性、較親水性之FA 為主。因為鴛鴦湖集水區深厚的有機質層下方為不易滲水的 黏土礦物層,故集水區的水流路徑應大部分為經過表土有機質層而逕流至湖中;
土壤有機質層中含量豐富、較接近湖水分子量的黃腐酸,可能即為湖水DOC 的 重要來源。杜鵑、蘚凋落物之淋洗液,其pH 值、DOC 分子量及螢光光譜(EEMs) 與湖水較為接近,顯示林下灌木或苔蘚等附生植物之淋洗液可能為土壤有機質層 中的黃腐酸的重要來源。
3. 在鴛鴦湖湖區,植物之凋落物在土壤中可能因分解緩慢而大部分變成腐植物 質,儲存於土壤有機質層中;短期之大量降雨可能無法加快凋落物之分解,但可 能從表土的有機質層和附生植物之基質中沖淋出大量的腐植物質(特別是較親水 性的黃腐酸)而流入湖中,因而影響湖水之 pH 值和水色等。鴛鴦湖湖區的降雨量 為影響湖水DOC 濃度、pH 值、水色的重要環境因子之一,這些變量間有高度之
相關性,可由關係式相互推估彼此之值。鴛鴦湖容積小且位於低溫多雨地區,故 雨量對湖水水質的影響大、湖水的更新速率快,特別是颱風帶來大量的降雨經常 造成湖水劇烈的擾動、大量DOC 從鴛鴦湖集水區流出。採集前數天的降雨量較 能反應湖水水質的變動狀況。
4. 在雨量較少、淋洗作用較小時,鴛鴦湖湖水中的內源性、微生物性來源之 DOC (EEMs 圖之 B、T 峰)才較明顯。藻類生長時也會產生類似外源性腐植物質 之DOC (EEMs 圖之 A、C 峰),但因其在湖水中濃度很低,對湖水 DOC 之影響 不大。影響湖泊之初級生產力暨藻類之生長的因素非常多,本研究之藻類培養試 驗顯示:在營養鹽充足、DOC 濃度為 0~10 ppm 的培養條件下,鴛鴦湖湖水之 DOC 濃度愈高,則愈能促進藻類生長,顯示湖中藻類之數量變化與湖水中 DOC 含量有關。
六、 參考文獻
江殷儒。2002。鯉魚潭中葡萄藻代謝物質之生物毒性。國立臺灣大學植物學研究 所碩士論文。
行政院環境保護署環境檢驗所。2000。水中總有機碳檢測方法-過氧焦硫酸鹽加 熱氧化/紅外線測定法:NIEA W532.51C
周昌弘、吳俊宗、謝昱暲、彭鏡毅、邱志郁、楊棋明、黃元勳、高文媛。1998。
中央研究院植物研究所第四期五年發展計畫大型合作研究計畫三、植物歧異 度與生態系研究:研究成果摘要。中央研究院植物研究所。
劉麗純。2007。嘉南平原高砷地下水腐植物質的特性。國立臺灣大學地質學研究 所碩士論文。
Alberts, J. J., Takács, M., and Egeberg, P. K. 2002. Total luminescence spectral characteristics of natural organic matter (NOM) size fractions as defined by ultrafiltration and high performance size exclusion chromatography (HPSEC).
Organic Geochemistry 33:817–828.
Aoki, S., Ohara, S., Kimura, K., Mizuguchi, H., Fuse, Y., and Yamada, E. 2008.
Characterization of Dissolved Organic Matter Released from Microcystis aeruginosa. Analytical Sciences 24:389–394.
Chen, J. S. and Chiu, C. Y. 2000. Effect of topography on the composition of soil organic substances in a perhumid sub-tropical montane forest ecosystem in Taiwan. Geoderma 96:19–30.
Coble, P. G. 1996. Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy. Marine Chemistry 51:325–346.
Cole, J. J., Pace, M. L., Carpenter, S. R., and Kitchell, J. F. 2000. Persistence of net heterotrophy in lakes during nutrient addition and food web manipulation.
Limnology and Oceanography 45:1718–1730.
Cuthbert, I. D. and Giorgio, P. D. 1992. Toward a standard method of measuring color in freshwater. Limnology and Oceanography 37(6):1319–1326.
Eish, M. Y. Z. A. 2003. Characterization of humic substances using chemical,
chromatographic, and spectroscopic techniques. Doctoral dissertation, Tennessee Technological University, Tennessee.
Gorski, P. R., Armstrong, D. E., Hurley, J. P., and Krabbenhoft, D. P. 2008. Influence of natural dissolved organic carbon on the bioavailability of mercury to a
freshwater alga. Environmental Pollution 154:116–123.
Gross, E. M., Meyer, H., and Schilling, G. 1996. Release and ecological impact of algicidal hydrolysable polyphenols in Myriophyllum spicatum. Phytochemistry
41:133–138.
Houser, J. N. 2006. Water color affects the stratification, surface temperature, heat content, and mean epilimnetic irradiance of small lakes. Canadian Journal of Fisheries and Aquatic Sciences 63:2447–2455.
Körner, S. and Nicklisch, A. 2002. Allelopathic growth inhibition of selected phytoplankton species by submerged macrophytes. Journal of Phycology 38:862–871.
Lai, I. L., Chang, S. C., Lin, P. H., Chou, C. H., and Wu, J. T. 2006. Climatic
characteristics of the subtropical mountainous cloud forest at the Yuanyang Lake long-term ecological research site, Taiwan. Taiwania 51(4):317–329.
Libes, S. M. 1992. An Introduction to Marine Biogeochemistry. New York: John Wiley & Sons, p. 411.
Maie, N., Yang, C. Y., Miyoshi, T., Parish, K., and Jaffé, R. 2005. Chemical characteristics of dissolved organic matter in an oligotrophic subtropical wetland/estuarine ecosystem. Limnology and Oceanography 50(1):23–35.
Mobed, J. J., Hemmingsen, S. L., Autry, J. L., and Mcgown, L. B. 1996. Fluorescence characterization of IHSS humic substances: Total luminescence spectra with absorbance correction. Environmental Science and Technology 30:3061-3065.
Pace, M. L. and Cole, J. J. 2002. Synchronous variation of dissolved organic carbon and color in lakes. Limnology and Oceanography 47(2):333–342.
Prakash, A. and Rashid, M. A. 1968. Influence of humic substances on the growth of marine phytoplankton--Dinoflagellates. Limnology and Oceanography
13:598–607.
Qiu, S., McComb, A. J., Bell, R. W., and Davis, J. A. 2005. Estimating production of gilvin from catchment leaf litter during seasonal rains. Marine and Freshwater Research 56:843–849.
Rees, R., Chang, S. C., Wang, C. P., and Matzner, E. 2006. Release of nutrients and dissolved organic carbon during decomposition of Chamaecyparis obtusa var.
formosana leaves in a mountain forest in Taiwan. Journal of Plant Nutrition and Soil Science 169:792–798.
Sachse, A., Babenzien, D., Ginzel, G., Gelbrecht, J., and Steinberg, C. E. W. 2001.
Characterization of dissolved organic carbon (DOC) in a dystrophic lake and an adjacent fen. Biogeochemistry 54:279–296.
Schulten, H. R. and Leineweber, P. 2000. New insights into organic-mineral particles:
composition, properties and models of molecular structure. Biology and Fertility of Soils 30:399–432.
Senesi, N. 1990. Molecular and quantitative aspects of the chemistry if fulvic acid and its interaction with metal ions and organic chemicals. Analytica Chimica Acta
232:77-106.
Sierra, M. M. D., Giovanela, M., Parlanti, E., and Soriano-Sierra, E. J. 2005.
Fluorescence fingerprint of fulvic and humic acids from varied origins as viewed by single-scan and excitation/emission matrix techniques. Chemosphere
58:715–733.
Steinberg, C. E. W. 2003. Ecology of Humic Substances in Freshwaters. Determinants from Geochemistry to Ecological Niches. Springer, Berlin.
Steinberg, C. E. W., Kamara, S., Prokhotskaya, V. Y., Manusadžianas, L., Karasyova, T. A., Timofeyev, M. A., Jie, Z., Paul, A., Meinelt, T., Farjalla, V. F., Matsuo, A. Y.
O., Burnison, B. K., and Menzel, R. 2006. Dissolved humic substances –
ecological driving forces from the individual to the ecosystem level? Freshwater Biology 51: 1189–1210.
Steinberg, C. E. W., Paul, A., Pflugmacher, S., Meinelt, T., Klöcking, R., and Wiegand, C. 2003. Pure humic substances have the potential to act as xenobiotic
chemicals – A review. Fresenius Environmental Bulletin 12:391–401.
Sun, B. K., Tanji, Y., and Unno, H. 2005. Influences of iron and humic acid on the growth of the cyanobacterium Anabaena circinalis. Biochemical Engineering Journal 24:195–201.
Takeda, H. 1995. A 5-year study of litter decomposition processes in a Chamaecyparis-obtusa Endl. forest. Ecological Research 10:95–104.
Thurman, E. M. 1985. Organic Geochemistry of Natural Waters. Martinus Nijhoff/Dr W. Junk Publishers, Dordrecht.
Thurman, E. M. and Malcolm, R. L. 1981. Preparative isolation of humic substances.
Environmental Science and Technology 15(4):463-466.
Tsai, J. W., Kratz, T. K., Hanson, P. C., Wu, J. T., Chang, W. Y. B., Arzberger, P. W., Lin, B. S., Lin, F. P., Chou, H. M., and Chiu, C. Y. 2008. Seasonal dynamics, typhoons and the regulation of lake metabolism in a subtropical humic lake.
Freshwater Biology 53:1929–1941.
US Environmental Protection Agency. 1992. In vitro determination of chlorophyll a and pheophytin a in marine and freshwater phytoplankton by fluorescence.
Method 445.0. Cincinnati, Ohio.
Wu, J. T., Chang, S. C., Wang, Y. S., Wang, Y. F., and Hsu, M. K. 2001.
Characteristics of the acidic environment of the Yuanyang Lake (Taiwan).
Botanical Bulletin of Academia Sinica 42:17–22.
Yamashita, Y. and Tanoue, E. 2003. Chemical characterization of protein-like fluorophores in DOM in relation to aromatic amino acids. Marine Chemistry 82:255–271.