維生素 C 對過氧化氫誘導氧化模型的測試依照不同時 間以及反應 之先後順序所測得的結果如下 (Nikon eclipse TS100 目鏡 20x；物鏡 40x):
4.3.1 維生素C 10 mM 對細胞抗氧化測試
H2O2與維生素C 對細胞的作用時間測試：
30min
30min
30min 細胞形態
1h 細胞形態
2h 細胞形態
3h 細胞形態
4h 細胞形態 加入維生素C 0o內縮 0o內縮 0o內縮 0o內縮 0o內縮
加入H2O2 0o 0o 0o 0o 0o
加入 H2O230min-4h 0o 加入維生素C 20mM 30min-4h 0o
結果顯示：加入 H2O2 後 4 個小時對細胞形態無明顯影響，損傷程度為 0o；但加入 20 mM 維生素C 30 min之後，細胞開始出現形態收縮的現象，
但並非受到氧化攻擊，判斷為 20mM 維生素C 溶液對細胞而言為高張溶 液，導致細胞形態內縮，損傷程度為 0o。
H2O2組加入維生素C 對細胞形態測試 (先以H2O2培養再加入維生素C ) 培養 12 小時後之形態：
30min
30min
之先後順序所測得的結果如下 (Nikon eclipse TS100 目鏡 20x；物鏡 40x):
加入 H2O2 培養 30min-4h 後 未加維生素E 3o
加入維生素E 培養 30min-4h 後 未加 H2O2 0o 之先後順序所測得的結果如下(Nikon eclipse TS100 目鏡 20x；物鏡40x):
H2O2與 β-carotene 對細胞的作用時間測試：
加入 H2O2 30min-4h 0o 加入 β-carotene 2h-4h 0o內縮
加入 H2O2 培養 30 min-4h 後 未加 β-carotene 3o 再加入β-carotene 1o-2o
結果顯示：加入 H2O2 後再加入 β-carotene，細胞呈現嚴重內縮且甚至破 裂死亡，但形態與受氧化壓力明顯不同，氧化損傷程度為1o-2o，判斷是由 於過高濃度的β-carotene 所導致；而未加 β-carotene 的對照組則皆受到氧 化攻擊，形態呈現皺縮，氧化損傷程度為3o。
加入 β-carotene 培養 30min-1h 未加 H2O2 0o嚴重內縮 後再加入 H2O2 0o
結果顯示：加入 β-carotene 後再加入 H2O2 可發現 30 min 至 1h 內抗氧 化效果最好，細胞雖因高濃度 β-carotene 產生內縮但未發現有受氧化攻 擊的現象，氧化損傷程度為0o；2h 至 4h 細胞逐漸減少，但整體而言細 胞型態相較於先加 H2O2 再加入 β-carotene 之實驗組為佳，氧化損傷程 度為1o；而未加 H2O2 的對照組細胞型態皆發生內縮部份甚至破裂死亡 ， 判斷為高濃度 β-carotene 所導致，氧化損傷程度為0o。
4.6 藻藍素抗氧化能力分析
在經過藻藍素、維生素C 、維生素E 以及 β-carotene 對細胞氧化壓 力的實驗後結果歸納如下(藻藍素、維生素C、維生素E 以及 β-carotene 濃 度皆為 10mM；H2O2 濃度為 0.03%；表格結果為培養12h後)：
單獨加入各種抗氧化劑與過氧化氫之形態比較 ：
30min
先加入 H2O2 再加入抗氧化劑測試抗氧化力的實驗顯示：藻藍素相較其他
先加抗氧化劑再加入 H2O2 的實驗顯示：除了 β-carotene 雖因濃度高造 成細胞內縮，但在 1h 內並無受到氧化攻擊的現象，氧化損傷程度為0o， 2h 之後細胞才逐漸破裂死亡，判斷 β-carotene 先加入 1h 內可有效抑制 氧化壓力，而超過 1h 之後則因細胞持續處於高張溶液下，造成細胞開始 形態改變；而其他抗氧化劑無論先作用細胞多久時間，當 H2O2 加入，
細胞還是無法抑制所造成的氧化壓力 ，氧化損傷程度為2o - 4o。由此可知 當細胞受到氧化性攻擊時，在特定時效內進行抗氧化作用，才可達到抗氧 化效果。
陸、結論
果，由此可知抗氧化劑若先加入或超過了有效時間，則無法有效抑制氧化 壓力，因種類而異。
9.由此實驗可得知，當身體細胞受到外來的氧化攻擊時，必須儘早做抗氧 化的動作，例如食用富含藻藍素、維生素等食品，若超過時間，抗氧化效 果則不明顯。
10.本實驗可作為Lyngbya sp.內所含有的藻藍素抗氧化能力 基礎的研究，
在濃度 10mM 下，抗氧化的能力即優於一般抗氧化劑，證明Lyngbya SP.
所萃取出的藻藍素日後有極高的商業價值 。
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附錄一、C-10 培養基配方
C-10 培養基 (藥品單位為每公升蒸餾水中所含公克數 : g/L )
HEPES(N-2-hydroxyethyl-piperazine-N’-2-ethanesulfonic acid ) 1.2 g/L
MgSO4·7H2O 0.25 g/L
K2HPO4 0.05 g/L
Ca(NO3)2·4H2O 0.025 g/L
KNO3 1.0 g/L
Na2-EDTA(Na2-ethylenediaminetetraacetic acid) 0.01 g/L
Fe2(SO4)3·6H2O 0.004 g/L
微量元素 1 ml/L
微量元素 (藥品單位為每公升蒸餾水中所含公克數 : g/L )
H3BO3 2.86 g/L
MnCl2·4 H2O 1.81 g/L
ZnSO4·7H2O 0.222 g/L
MoO3(85%) 0.018 g/L
CuSO4·5H2O 0.079 g/L
CoCl2·6H2O 0.01 g/L