Acetic acid (%) pH 值
0 7.4
0.05 6.17 0.1 4.84 0.2 4.55 0.4 3.86 0.8 3.48
表 一、醋酸濃度其相對應的酸鹼值
Strain
Light dose (J /cm2)
Erythrosine concentration
Gram-positive S. aureus
37.5 100 μM
50 50 μM
S. mutans
25 100 μM
50 25 μM
Yeast C. albicans 50 4 mM
表 二、赤蘚紅對懸浮菌體達到光動力作用撲殺全部菌體的條件
圖 一、赤蘚紅(Erythrosine)的結構54
圖 二、生物膜反應器及裝置67 H2O
圖 三、綠色發光二極體矩陣
圖 四、赤蘚紅(10 μM)在 PBS 溶液中之紫外光-可見光光譜。
UV-vis spectrum of erythrosine in PBS
0.0 0.2 0.4 0.6 0.8 1.0
450 500 550 600
Wavelength (nm)
Absorbace
Day 1 Day 3 Day 5 Day 7 Day 14 Day 21 Day 28
Bright Field Fluorescence
Control
10 min
30 min
圖 五、白色念珠菌與赤蘚紅(20 μM)作 10 及 30 分鐘，以螢光顯微鏡觀察 之影像。
Bright Field Fluorescence
Control
10 min
圖 六、白色念珠菌生物膜與赤蘚紅(20 mM)作用 10 分鐘，以雷射共軛焦顯微 鏡觀察之影像。
0.0
cps per bacterium (1x106 ) Erythrosine 10 μM
圖 七、白色念珠菌與赤蘚紅(20 μM)作用10及30分鐘後，以螢光光
0 菌結果。(金黃葡萄球菌：108 CFU / mL；赤蘚紅：0.01、0.025、0.05、
0.1、1 mM；光照劑量：37.5 (■)、50 ( □) J/cm2)。所有數據均是三
0
0 1 2 3 4 5 6 7 8 9
control 0 1 5 10 15 20 Erythrosine (mM)
Log (CFU/mL)
圖 十三、赤蘚紅(0~20 mM)對大腸桿菌懸浮細胞進行光動力作用之殺菌結果。(大 腸桿菌：108 CFU / mL；赤蘚紅：0、1、5、10、15 及 20 mM；光照劑 量：0(■)、50(□) J/cm2)。所有數據均是三次獨立實驗的平均值。
(A) 革蘭氏陽性菌細胞壁結構
(B) 革蘭氏陰性菌細胞壁結構
圖 十四、革蘭氏陽性菌(A)及革蘭氏陰性菌(B)細胞壁結構圖
0
Acetic acid (%)
Log (CFU/mL)
0
0
0
0
Erythrosine conc. (mM) Log (CFU/cm2 )
0 Erythrosine conc. (mM)
Log (CFU/cm2 )
Erythrosine conc. (mM)
L og ( C F U /c m
2)
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