毒物、環境賀爾蒙、抗氧化物質、孕婦忌服類藥物等，將能更近一步確認這些民間 常用物質或藥材的安全性。
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圖 1 墾丁國家公園管理局頒發的保育類植物水沅花合法採集證
圖 2 水沅花生長在海岸潮間帶邊的珊瑚礁岩地形
圖 3 水沅花的植株近照
（A） （B）
（C）
圖 4 胚胎幹細胞 ES-D3 和 STO 飼細胞共同培養，ES-D3 具有團塊的形狀，和 STO 飼細胞間具有明顯的邊界（A）40X（B）100X（B）200X
(A) (B)
(C)
圖 5 BALB/3T3 Clone A1 細胞的生長情形（A）40X（B）100X（B）200X
（A） （B）
（C） （D）
（E）
圖 6 ES-D3 胚胎幹細胞在低貼附性培養皿上，以懸滴式培養培養三天後聚集成類 胚體（embryoid body）團（A）40X（B）100X（C）200X （D）製備懸滴式培 養小滴（E）懸滴式培養胚胎幹細胞
（A） （B）
(C)
圖 7 類胚體（embryoid body）團貼附在培養皿後，幹細胞開始遷移並繼續分化 的情形（A）第 1 天（B）第 3 天(C)第 5 天
圖 8 以光學顯微鏡檢測由胚胎幹細胞分化出來，具有膊動性特性的心肌細胞
（A） （B）
圖 9 以 WST-1 分析水沅花萃取物對 3T3 細胞和胚胎幹細胞的生長影響情形與毒性 效應（A）3T3 細胞（B）胚胎幹細胞
圖10 水沅花萃取液添加到細胞培養液中，高濃度的水沅花萃取液會促進 ES-D3 胚胎幹細胞分
化成心肌細胞，低濃度責反而會抑制心肌細胞的分化。
圖11 水沅花萃取液添加到細胞培養液中，ES 和 3T3 細胞種植密度為 1*104/mL，取 100uL 種
在96 wells，培養十天後以 WST-1 進行細胞毒性試驗，結果顯示低濃度的水沅花具有增殖體細胞
的作用，高濃度卻有毒殺幹細胞和體細胞的作用。
表1 水沅花影響胚胎幹細胞分化的配置方法與結果記錄
1 2 3 4 5 6 7 8 9 10 水沅花萃
取液（uL） 50uL=x/20 x/21 x/22 x/23 x/24 x/25 x/26 x/27 0 0 PBS 0 50-25/20 50-25/21 50-25/22 50-25/23 50-25/24 50-25/25 50-25/26 50 uL
medium 950 950 950 950 950 950 950 950 950 1000 uL 心肌分化
率（％） 90 95 100 85 60 60 70 10 40 50 跳動強度 強 強 強 強 中 中 中 中 微弱 微弱
結構 平貼 平貼 平貼 稍立體 較立體 高聳 高聳 腔室狀 平貼 平貼