internal control LC3b LC3b-II/
LC3b-I
4.2.1 Iba-1
Iba-1 4 5 6
( p=0.4712 p=0.0927
p=0.2980) 8
4.2.2 LC3b
LC3b-II/LC3b-I LC3b 7
8 9 (
p=0.2298 p=0.4712 p=0.2298) 9
4.2.3 Aβ42
Aβ42 10 11 12
( p=0.0927 p=0.9362
4.3
(Shackelford, Long et al.
2002) (slight)
(minimal)
12 16 17 18 19
24
MDA Iba-1 LC3b Aβ42
Tau
MDA
5.1
15-30 nm
300 2000 µg/m3
SRM 2975 SRM 1650 SRM 2975
SRM 1650
DEP
SRM 2975 81.69 ng/mg 9454 ng/µg
SRM 1650b SRM 2975 2~3 (202.1 ng/mg)
(2011 ng/µg)(Bengalli, Zerboni et al. 2019)
SRM 2975 SRM 1650b ROS RNS
(Bonvallot, Baeza-Squiban et al. 2001, Totlandsdal, Herseth et al. 2012, Totlandsdal, Låg et al. 2015) PAH
SRM 1650b
PBS
PBS
SRM 1650b
5.2
3xTg-AD
6-8
C57BL/6 C57BL/6
BALB/c Sprague Dawley
Maja Hullmann 5XFAD
0.95 mg/m3
Aβ42 (Hullmann, Albrecht et al. 2017)
3xTg-AD
3xTg-AD
C57BL/6
6 12 18
3xTg-AD
6 3xTg-AD
(Villamil-Ortiz and Cardona-Gomez 2015) 3xTg-AD
3xTg-AD 2
(Belfiore, Rodin et al. 2019) 3xTg-AD
3xTg-AD 12
3xTg-AD
744 450 3xTg-AD
(Rae and Brown 2015)
5.3 5.3.1
MDA HNE MDA
MDA MDA
Cole C57BL/6
250-300 µg/m3 6 MDA
(Cole, Coburn et al. 2016)
MDA C57BL/6
MDA
(2-thiobarbituric acid reacting substances test, TBARS assay)
(Thiobarbituric acid, TBA)
MDA MDA-TBA (Spectrophotometric
assay)
TBA MDA-TBA
(Meagher and FitzGerald 2000) TBARS
MDA DNPH
high-performance liquid chromatography (HPLC)
MDA(Cordis, Das et al. 1998, Pilz, Meineke et al. 2000)
TBA DNPH MDA TBA
(Ceconi, Cargnoni et al. 1991, Pilz, Meineke et al. 2000, Tukozkan,
Erdamar et al. 2006) HPLC-MS/MS DNPH MDA
MDA-DNPH
(
Dideuterated MDA, D2-MDA) MDA (Mendonça, Gning et al.
2017)
5.3.2
Iba-1
Iba-1
1α, IL-1β, TNFα, IL-6
DEPs Iba-1
50 µg 100 µg
150 µg 300 µg IL-1β, TNFα, IL-6
50 µg
M1 M2
M1 IL-1β,
IL-6, IL-12, TNF-α
M1 M2
M1 M2
M2 M1 (Jimenez, Baglietto-Vargas et al. 2008) Iba-1
M1 CD36, CD14, CD11c, MHC-II
M1 iNOS, TNFα, NADPH
MHC-II
(IHC) Iba-1 CD68
5.3.3
LC3b
(lipopolysaccharide, LPS)
(François, Terro et al. 2014)
LC3
LC3 16 kDa LC3-I 14
kDa LC3-II LC3b-1 LC3b-11
(Mizushima 2004)
LC3-II/LC3-I LC3 LC3-II
LC3
(autophagic flux) (Klionsky,
Abdelmohsen et al. 2016) LC3
Beclin-1 p62
Western blot LC3-II LC3-I LC3-II LC3-I
LC3
LC3-I LC3-II
LC3-II(Chu, Plowey et al. 2009) LC3-I LC3-II SDS
LC3-I LC3-II
(Mizushima and Yoshimori 2007) LC3-I
LC3-II ( tubulin β-actin)
LC3 (Klionsky, Abdelmohsen et al. 2016, Yoshii and Mizushima 2017)
5.3.4
42 Aβ42 (monomers)
(oligomers) (fibrils) (plaques)
Aβ Aβ (Walsh, Klyubin et al.
2002, Kayed, Head et al. 2003) Aβ42
Tau (p-tau)
Tau (Liazoghli, Perreault et al. 2005)
Tau (Chesser, Pritchard et al. 2013) Tau
Tau Tau
Aβ Tau
Aβ Tau
1000µg/m3
Aβ42 Tau
5XFAD
Aβ C57BL6
t-Tau p-Tau 3xTg-AD
5.3.5
H&E
( 2016, 2017, 2018)
(Kim, Kabir et al.
2015)
(Levesque, Surace et al. 2011)
(Hartz, Bauer et al. 2008, Levesque,
Taetzsch et al. 2011) (Kim, Lee et al.
2016)
Aβ Tau 3xTg-AD
C57BL/6
12 3xTg-AD
24
(Bovine Serum Albumin, BSA) 20 (Tween-20)
Tau
(Immunohistochemistry, IHC) (Congo Red)
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