The nitrogenous and carbonaceous compounds present in the aeration tank were removed by the SNAD process. The overall removal efficiencies of nitrogenous and carbonaceous compounds in the SNAD process was calculated using stoichiometric equations are 75.7% and 28%, respectively. The microorganism from the landfill-leachate treatment aeration tank was confirmed to be Anammox bacteria using the FISH and PCR analyses.
The SNAD process also was successfully developed in a SBR. The influence of OLR and NLR on the performance of the SBR was investigated. The experimental outcomes indicate that the performance of the SBR is affected more by the NLR compared to the OLR. The influent BOD has a good correlation with the TN removal in the system. Partial nitrification and anammox were responsible for majority of total nitrogen removal in the SBR. The landfill-leachate contains less bioavailable COD; therefore, the effluent from the SNAD process still needs some treatment with regard to organics. The maximum ammonium removal in the SBR was close to 94%, however, it can be improved by increasing the oxygen-transfer efficiency from gas to liquid phase. As a whole, the experimental findings indicate that the SNAD process is more suitable for the landfill-leachate treatment with high nitrogen and low COD content.
Recommendations for future work
Based on the findings of this study, some suggestions are summarized as follows:
1. The reactor used for SNAD process could be improved by sealing the reactor exposing to the atmosphere. A completely covered SBR is a better reactor design for estimating the gas production (nitrogen gas, nitrous oxide) and oxygen aerating rate.
2. The model based on simple assumptions could be improved through consideration of more detailed aspects.
57
3. Continuous flow of a reactor design could be implemented to ehance the performance in the future study.
4. According to the slowly growth of Anammox bacteria, attached growth could provide Anammox bacteria retaining in the reactor and reduced the DO exposure chance.
5. The ratio and quantity of AOB, NOB, Anammox bacteria and heterotrophic denitrifier could be identified using biological molecular technologies. The results of this examination could explain the performance of the SNAD process.
6. An appropriate HRT and SRT might be estimated in the future study.
58
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Appendix A: 16S rRNA sequence tggcg aaagggtgag
taatgcattg ataacctgcc tttgagatgg gaataactgc gtttcgagca atcggaacta ccgaaagggc tgctaatacc caataatact ataggtgcaa aagcacttgt ggtcaaatgc taggaattct gttccttgtg cttaaagagg ggttaatgtc ctatcagcta gttggtgggg taatggccta ccaaggcaaa gacgggtagc cggcttgaga gggtggtcgg ccacactggg actgagacac tgcccagact cctacgggag gctgcagtcg agaatctttc gcaatgcccg aaagggtgac gaagcgacgc cgcgtgtggg aagaaggcct tcgggttgta aaccactgtc gggagttagg aaatgcaggt gcgttaatag cgcacttgct tgactaaggc tccagaggaa gccacggcta actctgtgcc agcagccgcg gtaatacaga ggcggcaagc gttgttcgga attattgggc gtaaagagca cgtaggcggc cttgcaagtc agttgtgaaa gccttccgct taacggaaga acggcatctg atactacagg gcttgagtac gggaggggag agtggaactt ctggtggagc ggtgaaatgc gtagatatca gaaggaacgc cggcggcgaa agcgactctc tggtccgaaa ctgacgctga gtgtgcgaaa gctaggggag caaacgggat tagatacccc ggtagtccta gccgtaaacg atgggcacta agtagagggg ttttgattat ttctctgccg gagctaacgc attaagtgcc ccgcctgggg agtacggccg caaggctaaa actcaaaaga attgacgggg gctcgcacaa gcggtggagc atgtggctta attcgatgca acgcgaagaa ccttaccggg gcttgacatg gtagaagtag aatcctgaaa gggtgacgat cggtatccag tccgaagcta tcacaggtgt tgcatggctg tcgtcagctc gtgtcgtgag acgttgggtt aagtccccta acgagcgaaa cccttgtctt tagttgctaa cgggtaatgc tgagcacttt agagagactg ccgtcgttaa gacggaggaa ggtggggacg acgtcaagtc atcatggccc ttatgtcccg ggctgcacac gtgctacaat ggtcggtaca aagggatgct aagtcgcgag atggtgcgaa acctataaag ccgatctcag ttcagattgg aggctgaaac tcgcctccat gaagttggaa tcgctagtaa tcgcggatca gctacgccgc ggtgaatatg ttcccgagcc ttgtacacac cgcccgtcaa gccacccaag caagatgcac ccaaaatcgc ctgcctaacc cgtaagggag ggaagtgcct aagg
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Appendix B: The original data of SBR
Time
73
74
75
76
77
78
79
80
81
82
258 12.615 7.3 154 319 1.5
259 29.537 7.12 162 319 140 4.4 90 152 1.6 410 23 16
260 58.775 7.07 177 319 0
261 11.738 7.37 -34 319 138 2.8 180 187 0 500 66 55
262 1.183 7.35 -18 319 0
263 1.979 7.6 -10 319 181 2.4 96 173 0 490 68 43
264 3.756 7.24 -4 319 0
265 5.431 7.68 -17 319 0
266 1.285 7.42 -1 319 232 0.4 80 168 0 470 57 38