Curriculum Vitae
Ming Chi Yang (楊明誌), Ph.D.
Date of Birth: October 10, 1981
Institute of Biochemical Engineering, 國立交通大學 National Chiao Tung University 生化工程研究所 75 Po-Ai Street, 新竹市博愛街 75 號 Hsinchu 30050, Taiwan. 生科實驗館 101 室
Tel: +886-3-571-2121 ext. 56939 聯絡電話：03-5712121 ext.56939 Mobile Tel: +886-9-32936291 手機：0932936291
Fax: +886-3-572-9288 傳真：03-5729288 E-mail: [email protected]
Education
2005/9-present Ph.D. Institute of Biochemical Engineering, National Chiao Tung University, Hsinchu, Taiwan (國立交通大學生化工程研究所)
2004/9-2005/6 M.S. Institute of Biochemical Engineering, National Chiao Tung University, Hsinchu, Taiwan (國立交通大學生化工程研究所)
1999/9-2004/6 B.S. Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan (國立清華大學生命科學系)
Dissertation
Physiological and physico-biochemical function of bovine milk β-lactoglobulin as probed by site-directed mutagenesis, bioinformatics and its crystal structure complexed with vitamin D Advisor: Chair Professor Simon J.T. Mao, Ph.D. (毛仁淡 講座教授)
This work constitutes identification of the secondary vitamin D binding site (exosite) of β-lactoglobulin, essential residues for exosite binding and vitamin D transport role of β-lactoglobulin. Most detail of the dissertation has been published as journal articles (see publication list).
Research Area
Doctoral and Graduate Research
Institute of Biochemical Engineering, National Chiao Tung University, Hsinchu, Taiwan, 2004/9-2009 (research advisor: Dr. Simon J.T. Mao). (指導教授：毛仁淡 講座教授)
z Identification of the secondary vitamin D binding site (exosite) of β-lactoglobulin by fluorescence spectra, bioinformatic programs and X-ray diffraction.
z Determination of essential residues for exosite binding by site-directed mutagenesis and monoclonal antibody.
z Definition of vitamin D transport role of β-lactoglobulin with animal study.
z Ligand binding ability of β-lactoglobulin in native and thermal denatured state.
z Simulation of binding between haptoglobin and hemoglobin.
Undergraduate Research:
Division of Animal Medicine, Animal Technology Institute Taiwan, Maioli, Chunan, Taiwan, 2001/9-2003/6 (research advisor: Dr. Chao-Wei Liao). (指導教授：廖朝暐 研究員)
z Immune response of pig after immunization with alive vaccine of porcine reproductive and respiratory syndrome (PRRS) virus and a chimeric protein composed PRRSV ORF6 with Pseudomonas exotoxin A.
z Development of oral T-cell vaccine for porcine reproductive and respiratory syndrome (PRRS) virus.
Teaching Experience
Teaching Assistant “Biochemical Experiments”
Department of Biological Science and Technology, National Chiao Tung University, September 2006-January 2007
Teaching Assistant “Organic chemistry”
Department of Biological Science and Technology, National Chiao Tung University, February 2006-June 2006
Teaching Assistant “Drug delivery”
Department of Biological Science and Technology, National Chiao Tung University, September 2005-January 2006
Technical Experience
Molecular genetics: Genomic DNA and total RNA isolation; PCR and RT-PCR;
Electrophoresis of nucleic acids; Gene cloning and expression; Site-directed mutagenesis.
Biochemistry: Polyacrylamide gel electrophoresis; Protein determination;
Spectrophotometric enzyme assays; Microencapsulation; Affinity (monoclonal antibody) column chromatography; HPLC (gel filtration, ion exchange and reverse phase); Ligand binding assay by fluorescence spectrophotometer and isothermal calorimetry; Peptide synthesis; Protein array; Chemical modification of protein; Limited proteolysis;
MALDI-TOF mass spectra; Confocal laser scanning microscopy; Protein crystallography.
Immunohistochemistry; Fluorescent and enzyme labeling of antibody and protein; FACS analysis.
Professional Certificate
z Certificate in radiation safety training for operator (Radiation Protection Association R.O.C.) 「財團法人中華民國輻射防護協會操作人員輻射安全訓練班結訓證書 (94 輻 協訓字第 0076 號」
z Certificate in ethical training in animal experimentation (Council of Agriculture, Executive Yuan, Taiwan) 「行政院農業委員會實驗動物人道管理訓練合格證書 (實動訓字(九四) 第一九○號」
Publications
1. Song CY, Chen WL, Yang MC, Huang JP, Mao SJT. Epitope mapping of a monoclonal antibody specific to bovine dry milk. Involvement of residues 66-76 of strand D in thermal denatured β-lactoglobulin. Journal of Biological Chemistry 2005; 280:
3574-3582. (SCI IF: 5.6; Area of Biochemistry & Molecular Biology: 40/263)
2. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. A novel conformation-dependent monoclonal antibody specific to native structure of β-lactoglobulin and its application.
Journal of Dairy Science 2006; 89:912-921. (SCI IF: 2.4; Area of Agriculture, Dairy &
Animal Science: 3/47)
3. Yang MC, Guan HH, Liu MY, Yang JM, Chen WL, Chen CJ, Mao SJT. Crystal structure of a secondary vitamin D3 binding site of milk β-lactoglobulin. 2006 Protein Data Bank (PDB) access code: 2GJ5 (MMDB ID: 58939).
4. Yang MC, Guan HH, Liu MY, Lin YH, Yang JM, Chen WL, Chen CJ, Mao SJT. Crystal structure of a secondary vitamin D3 binding site of milk β-lactoglobulin.
Proteins-Structure Function and Bioinformatics 2008; 71:1197-1210. (SCI IF: 4.7 based on the year submitted; Area of Biophysics: 18/69)
5. Lai IH, Lin KY, Larsson M, Yang MC, Shiau CH, Liao MH, Mao SJT. A unique tetrameric structure of deer plasma haptoglobin: An evolutionary advantage in HP 2-2 phenotype with homogeneous structure. FEBS Journal 2008; 275: 981-993. (SCI IF: 3.4;
Area of Biochemistry & Molecular Biology: 91/263)
6. Yang MC, Guan HH, Yang JM, Ko CN, Liu MY, Lin YH, Huang YC, Chen CJ, Mao SJT.
Rational design for crystallization of β-lactoglobulin and vitamin D3 complex: revealing a secondary binding site. Crystal Growth & Design 2008; 8: 4268-4276 (SCI IF: 4.0; Area of Crystallography: 1/25). Selected as the cover of Journal Issue 2008, Dec.
7. Yang MC, Chen NC, Chen CJ, Wu CY, Mao SJT. Evidence for β-lactoglobulin involvement in vitamin D transport in vivo- role of the γ-turn (Leu-Pro-Met) of β-lactoglobulin in vitamin D binding. FEBS Journal 2009; 276: 2251-2265 (SCI IF: 3.4;
Area of Biochemistry & Molecular Biology: 91/263)
8. Yang MC. Physiological and physico-biochemical function of bovine milk β-lactoglobulin as probed by site-directed mutagenesis, bioinformatics and its crystal structure complexed with vitamin D. 2009, Ph.D. thesis in the Institute of Biochemical Engineering, National Chiao Tung University, Hsinchu. Taiwan.
Presentations
1. Yang MC, Chen WL, Mao SJT. Ligand binding ability of β-lactoglobulin in native and thermal denatured state. The Twentieth Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Oral presentation. Programs & abstracts. P230 (March 26-27, 2005) 2. Chen WL, Yang MC, Song CY, Mao SJT. Thermal denaturation of β-lactoglobulin as
probed by specific monoclonal antibodies. The Twentieth Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Programs & abstracts. P396 (March 26-27, 2005) 3. Yang MC, Chen WL, Mao SJT. Secondary vitamin D binding domain of β-lactoglobulin:
A thermal independent site. The 10th Conference on Biochemical Engineering, Taipei, Taiwan. Oral presentation. Programs & abstracts. P103 (June 24-25, 2005)
4. Mao SJT, Chen WL, Yang MC, Liu WT. Distinguish between native and thermally denatured β-lactoglobulin using a monoclonal antibody as a probe. 2005 Joint Annual Meeting of ADSA-ASAS-CSA, Cincinnati, OH, USA. Oral presentation. Programs &
abstracts. P77 (July 24-28, 2005)
5. Song CY, Yang MC, Mao SJT. Residues 69-74 of β-lactoglobulin are responsible for a monoclonal antibody binding to thermal denatured lactoglobulin. 2005 Joint Annual Meeting of ADSA-ASAS-CSA, Cincinnati, OH, USA. Oral presentation. Programs &
abstracts. P76 (July 24-28, 2005)
6. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. β-lactoglobulin plays a provocative role in lymphocyte proliferation. The Fourteenth Symposium on Recent Advances in Cellular and Molecular Biology, Pingtung, Taiwan. Programs & abstracts. P159 (January 18-20, 2006)
7. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. β-lactoglobulin plays a provocative role in lymphocyte proliferation. The Twenty-first Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Programs & abstracts. P120 (March 18-19, 2006) 8. Yang MC,Chen C J, Liu MY, Mao SJT. Crystal structure of β-lactoglobulin and vitamin
D complex. Identification of a second binding site for vitamin D. The Twenty-first Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Programs & abstracts. P167 (March 18-19, 2006)
9. Yang MC,Chen C J, Liu MY, Mao SJT. Crystal structure of β-lactoglobulin and vitamin
10. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. β-lactoglobulin plays a provocative role in lymphocyte proliferation. Experimental Biology 2006, San Francisco, CA, USA.
FASEB. J. 20 :A961 (April 1-5, 2006)
11. Guan HH, Yang MC, Liu MY, Yang JM, Chen WL, Mao SJT, Chen CJ. Crystal structure of β-lactoglobulin and vitamin D complex. NSRRC 12th Users’ Meeting and Workshops, Hsinchu, Taiwan. Oral presentation. Programs & abstracts. O3-1 (October 3-4, 2006) 12. Yang MC, Guan HH, Liu MY, Lin YH, Yang JM, Chen WL, Chen CJ, Mao SJT. Crystal
structure of a secondary vitamin D3 binding site of milk β-lactoglobulin. 2006 Best Poster Competition of College of Biological Science & Technology, NCTU, Hsinchu, Taiwan.
Programs & abstracts. P94 (November17, 2006)
13. Yang MC, Guan HH, Liu MY, Lin YH, Yang JM, Chen WL, Chen CJ, Mao SJT. Crystal structure of a secondary vitamin D3 binding site of milk β-lactoglobulin. 2007 NCTU Conference of Biological Technology, Hsinchu, Taiwan. Programs & abstracts. P9 (October 30, 2007)
14. Guan HH, Yang MC, Liu MY, Yang JM, Chen WL, Mao SJT, Chen CJ. Crystal structure of β-lactoglobulin and vitamin D complex. Identification of a second binding site for vitamin D: A thermal independent site. The 8th Conference of the Asian Crystallographic Association, Taipei, Taiwan Programs & abstracts. P06-074 (November 4-7, 2007)
15. Yang MC, Chen NC, Mao SJT. Physiological function of secondary vitamin D binding site on milk β-lactoglobulin. The Sixteenth Symposium on Recent Advances in Cellular and Molecular Biology, Pingtung, Taiwan. Programs & abstracts. P166 (January 23-25, 2008)
16. Lai IH, Lin KY, Larsson M, Yang MC, Shiau CH, Liao MH, Mao SJT. A unique tetrameric structure of deer plasma haptoglobin: an evolutionary advantage in hp 2-2 phenotype with homogeneous structure. The Sixteenth Symposium on Recent Advances in Cellular and Molecular Biology Pingtung, Taiwan. Programs & abstracts. P167 (January 23-25, 2008)
17. Yang MC, Chen NC, Lee J, Lai IH, Mao SJT. Interaction of deer Hp 2-2 with hemoglobin is superior to human Hp 2-2: an evolutionally advantage. The Sixteenth Symposium on Recent Advances in Cellular and Molecular Biology Pingtung, Taiwan.
Programs & abstracts. P170 (January 23-25, 2008)
18. Yang MC, Chen NC, Mao SJT. Physiological Function of Secondary Vitamin D Binding Site on Milk β-Lactoglobulin. The Twenty-third Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Oral presentation. Programs & abstracts. P82 (March 29-30, 2008)
19. Lai IH, Lin KY, Larsson M, Yang MC, Shiau CH, Liao MH, Mao SJT. A unique tetrameric structure of deer plasma haptoglobin: an evolutionary advantage in Hp 2-2 phenotype with homogeneous structure. The Twenty-third Joint Annual Conference of
Biomedical Sciences, Taipei, Taiwan. Oral presentation. Programs & abstracts. P102 (March 29-30, 2008)
20. Yang MC, Chen NC, Lee J, Mao SJT. Interaction of deer Hp 2-2 with hemoglobin is superior to human Hp 2-2: an evolutionally advantage. The Twenty-third Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Oral presentation. Programs &
abstracts. P83 (March 29-30, 2008)
21. Yang MC, Guan HH, Liu MY, Lin YH, Yang JM, Chen WL, Mao SJT, Chen CJ. Rational Design for Crystallization of β-Lactoglobulin and Vitamin D3 complex Reveal a Secondary Binding Site. The 12th International Conference on the Crystallization of Biological Macromolecules, Cancun, Mexico. Oral presentation. (May 6-9, 2008)
22. Yang MC, Chen NC Structural and functional importance of the secondary vitamin D binding of β-Lactoglobulin. 2008 Best Poster Competition of College of Biological Science & Technology, NCTU. Programs & abstracts. G1-21 (May 28, 2008)
23. Yang MC, Chen NC Binding of deer Hp 2-2 with hemoglobin is superior to human Hp 2-2: an evolutionally advantage. 2008 Best Poster Competition of College of Biological Science & Technology, NCTU. Programs & abstracts. G1-20 (May 28, 2008)
24. Lai IH, Lin KY, Yang MC A unique tetrameric structure of deer plasma haptoglobin: an evolutionary advantage in Hp 2-2 phenotype with homogeneous structure. 2008 Best Poster Competition of College of Biological Science & Technology, NCTU. Programs &
abstracts. G1-23 (May 28, 2008)
25. Lai IH, Yang MC, Shiau CH, Liao MH, Mao SJT. A unique tetrameric structure of deer plasma haptoglobin: an evolutionary advantage in Hp 2-2 phenotype with homogeneous structure. The Spring Symposium of the Taiwan Association of Veterinary Medicine and Animal Husbandry, Pingtung, Taiwan. Programs & abstracts. P78. (May 31, 2008)
26. Chen CJ, Guan HH, Yang MC, Mao SJT. Rational crystallization of β-lactoglobulin and vitamin D3 complex reveal a secondary binding site. The XXI Congress and Assembly of the International Union of Crystallography (IUCr2008), Osaka, Japan. Acta Cryst. A64:
C242-C243. (August 23-31, 2008)
27. Yang MC, Guan HH, Yang JM, Ko CN, Liu MY, Lin YH, Huang YC, Chen CJ, Mao SJT.
Rational design for crystallization of β-lactoglobulin and vitamin D3 complex: revealing a secondary binding site. 2008 NCTU Research Conference of Biological Technology, Hsinchu, Taiwan. Invited speech. (December 30, 2008)
28. Yang MC, Guan HH, Yang JM, Ko CN, Liu MY, Lin YH, Huang YC, Chen CJ, Mao SJT.
Rational design for crystallization of β-lactoglobulin and vitamin D3 complex: revealing a secondary binding site. The Seventeenth Symposium on Recent Advances in Cellular and Molecular Biology Pingtung, Taiwan. Programs & abstracts. P214 (February 11-13,
29. Chen WL, Chen CL, Chao LS, Peng JY, Lai IH, Yang MC, Wang LS. The novel biomarkers for non-small cell lung carcinoma (NSCLC) by comparative proteomics:
cytochrome c oxidase subunit Va is a potentially new biomarker for tumor invasion in NSCLC. The Twenty-fourth Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Oral presentation. Programs & abstracts. P64 (March 21-22, 2009)
30. Yang MC, Guan HH, Yang JM, Ko CN, Liu MY, Lin YH, Huang YC, Chen CJ, Mao SJT.
Rational design for crystallization of β-lactoglobulin and vitamin D3 complex: revealing a secondary binding site. The Twenty-fourth Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Oral presentation. Programs & abstracts. P66 (March 21-22, 2009)
31. Lai IH, Wang SH, Lee JW, Chen WL, Yang MC, Lin BR, Mao SJT. Neutrophils as one of the major haptoglobin sources in mastitic milk. The Twenty-fourth Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. Programs & abstracts. P374 (March 21-22, 2009)
Honor
1. Award for outstanding oral presentation in the 2005 10th Conference on Biochemical Engineering, Taipei, Taiwan. “Secondary vitamin D binding domain of β-lactoglobulin:
A thermal independent site.”
2. Excellent Research Award in the 2007 NCTU Conference of Biological Technology, Hsinchu, Taiwan. ”Crystal structure of a secondary vitamin D3 binding site of milk β-lactoglobulin.”
3. Outstanding Poster Award in the 2008 Best Poster Competition of College of Biological Science & Technology, NCTU. “Structural and functional importance of the secondary vitamin D binding of β-Lactoglobulin.”
4. Outstanding Research Award and Invited Speech in the 2008 NCTU Research Conference of Biological Technology, Hsinchu, Taiwan. “Rational design for crystallization of β-lactoglobulin and vitamin D3 complex: revealing a secondary binding site.”
Publications