1. Introduction
1.6 Framework of Research
1.5 Motivations and Aims of This Study
Hot springs, one of extreme environments that had been proven to be a reservoir of valuable knowledge to the past (earth history), present (microbial ecology) and future (bioprospecting and astrobiology), are worth being further explored and understood. In the past few decades, studies of this field in Taiwan have accumulated a certain amount of knowledge that provided a rough image of local hot spring ecosystems. However, more thorough and systematic researches on prokaryotic diversity are needed. In this study, the high-throughput sequencing technique was applied to obtain sufficient sequence data that facilitated a more comprehensive analysis on prokaryotic community structure in an acidic hot spring ecosystem.
1.6 Framework of Research
Samples of hot spring water were collected, concentrated and from which metagenomic DNAs were extracted for fosmid library construction and direct shotgun sequencing. Results of sequencing were analyzed for assessments of prokaryotic community composition, genome mapping and community gene function distribution. Prokaryotic community structure analysis and gene functions were the main topics of this study (blue and green boxes of Figure 1).
Prior to the sample collection, a general survey on the bacteria, archaea and nanoarchaea distribution of six candidate hydrothermal springs in TVG area was done (white box of Figure 1 and Appendix 1).
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2. Materials and Methods