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FIGURE S1 | The reactivity and specificity of anti-zPnpo antibodies. The cells extracts prepared from the E.coli with (+IPTG)/without (-IPTG) overexpressing the recombinant zPnpo used for antibodies generation was subjected to Western blotting with custom-made anti-zPnpo antibodies. E. coli strain HMS174(DE3) encompassing the plasmids Nus-zPnpo/pET43.1a was added with 0.6 mM IPTG in log phase and grown for 6 hours at 37°C. Approximate 5 ml bacterial culture was collected and sonicated. The supernatant of cell lysate containing 2.25 μg protein (per lane) was subjected to Western blot analysis.
FIGURE S2 | Whole-mount in-situ hybridization with zpnpo sense probe.
Embryos at the indicated stages were collected and subjected to WISH by probing with the DIG-labeled riboprobes complementary to the anti-sense strain of zpnpo coding sequence (sense probe) as described in Materials and Methods.
FIGURE S3–10 | The impeded heart development observed in zPnpo morphants. Tg(cmlc2:eGFP) embryos with (3-6; MO)/without(7-10; WT) zPnpo MO injection were video-recorded at 3 dpf for heart pumping. Diminished red blood cells, decreased heart rate, distorted heart tube and incorrectly positioned cardiac chambers were observed in zPnpo morphants.
FIGURE S11 | The impeded trunk development in zPnpo morphants was rescued by co-injecting pnpo mRNA and supplementing with PLP.
Embryos injected with 5 ng zPnpo MO at 1 to 2-cell stages were co-injected with 800 pg zpnpo or hPNPO mRNA (columns 4 and 5), or grown in embryo water containing 1 mM PLP, 1 mM PN, 0.1 mM PM, or 0.1 mM PL (columns 6-9). The embryos displaying body curvature, representing mild defect (A), and lack of tail, representing severe defect (B), were recorded at 4 dpf. The Reported are the averages of data from at least three independent repeats with n = 60~80 embryos for each group and expressed as mean ± SEM.
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Conflict of Interest Statement: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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