Cbp-1 mutant real-time data

Fig 49. Cbp-1 mutant real-time PCR data to gene expression

Over 80% of the cancers known produce, because p53 is lost badly at present.

P53 is important, because it has 3 functions:

1. Order cells not to grow again, is engaged in the work of mending damaged DNA attentively (growth arrest).

2. When being unable to mend, order cells to commit suicide.

3. Suppress the blood vessel hyperplasia (anti-angiogensis), Supply the cancer cell with a nutrient, and make the cancer cell unable to grow up, shift.

Produce the cancer, but destroy p53 .Celegans cbp-1 is similar to human P300/CBP

P300/CBP is activating of p53, by combining with p53 N end, and 382 acetylating lysine, increase p53 activate the ability that the gene expression. [32] Cbp-1 gene expression accords with expectancy. Cbp-1 gene expression is cancer negative related.The result infers the magnetic field correlate with cancer genes.

Chapter 4 Conclusion.

Our initial discovery is that we find normal C.elegans N2 behavior is unusual under magnetic field function. Later we combine with super paramagnetic nanoparticles and magnetic field in our experiment. The result is more remarkable. We suppose the magnetic field also influence other physiological mechanisms besides behavior. In numerous real-time PCR data are analyzed, we find out the gene correlated with magnetic field thoroughly. Biological mechanism and gene expression pathway to C.elegans about the magnetic field are we design more than one hundred primer pairs in order to analyze gene expression. Certainly there are a lot of topics worthy to be deep we have not finished yet. By our present experimental result, we think the magnetic field causes the influence on the gene and behavior on the living beings.

1. The magnetic field influences C.elegans behavior.

C.elegans creeps slowly under magnetic field function. Noted down and all shown the magnetic field influence behavior by statistical graph or video. Mostly correlate with nervous system about research of C.elegans behavior. Nervous system relevant pathological changes have many such as Alzheimer's disease it is a question that is worth going into seriously.

2. Magnetic field is induction of apoptosis

Showing from real-time PCR data, there is a lot of apoptosis, cancer, oxidation all correlate with magnetic field function. In order to confirm the result even more. We have concentrated resources on finding out ced-3 and ced-6 gene that apoptosis are correlated with.Cbp-1 gene is correlated with cancer. Because of cbp-1 mutant behavior data, the magnetic field and cancer direct relation that we can not come to a conclusion.

3. Apoptosis deficient mutant is insensitive to magnetic field

Ced-3 is important key of apoptosis pathway. Ced-3 begins function expression and then the living cell switch a succession of apoptosis mechanisms. We use mutant C.elegans to prove apoptosis deficient mutant is insensitive to magnetic field. Reference [33] echoes with our result.

(Fig50) .They also use mutant ced-3 and ced-6. Ced-3 alone mutant is not apt apoptosis. Ced-6 mutant is not relevant directly to apoptosis, but ced-3 and ced-6 pairs of mutant more difficult to apoptosis.

Ced-3 mutant is not sensitive to the magnetic field (our experiment)

Ced-6 is pathways to regulate cell corpse engulfment.Ced-6 does not participate in apoptosis directly [33]. Our results reflect by ced-6 behavior data proves ced-6 single mutant not to be obvious to the magnetic field influence. Certainly the result of ced-3 and ced-6 accord with expectancy .The magnetic field relates to apoptosis directly. We confirm the magnetic field cause apoptosis to the living beings.

Fig 50 Reference [33] some data

Future prospect

Relevant researches about magnetic field influence living beings are worth our further investigating very much. We can find out the physiological factor that the magnetic field influences to C.elegans in succession in the future. We can make C.elegans life span. Still we prove other gene magnetic fields are related more accurately. We have proved 3 genes after all.

In addition, the proteins that the magnetic field related will be proved in the direction of our future.

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