• 沒有找到結果。

Potential Vectors of Pierce’s Disease in Taiwan: Ecology and Integrated Management

3. Educational promotion

In order to increase farmers’ awareness of the knowledge regarding PD symptoms and the favorable environment for potential insect vectors, Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ) invites Su and Shih to hold informational meetings about the integrated management of PD and potential insect vector every year.

So farmers can observe and cut down their infected grapevines, as well as prevent the host plants of potential insect vectors. At Bai-mao-tai 60 studying site, for example, after weed control by the farmers, the population of potential insect vectors was significantly reduced, and the numbers of PD-infected grapevines also continued to decline each year.

Future Prospects

PD was first reported in Taiwan in 2002. Since then, the researchers under the suggestion and support of the government performed immediate eradication of infected grapevines. Later, a cooperation between Taiwan Agricultural Chemicals and Toxic Substance Research Institute and Taiwan Agricultural Research Institute was established to study PD and the ecology of the leafhopper vectors, set up the management of potential insect vectors at different agriculture environments, investigate the hosts of pathogens and potential insect vectors, and reduce the population densities of pathogens and potential insect vectors in fields, thereby reducing economic losses.

Unfortunately, over the past decade, the researches on insect-mediated diseases and insect vectors received limited governmental funding. The studies of the potential insect vectors of PD in Taiwan, for example, require research works on technologies about monitoring migration of insect vectors and their feeding behavior, test of disease transmission, statistical population ecology, biological control, microbial control and new non-chemical materials. Therefore, it is urgent to incorporate domestic or international interdisciplinary collaborative researches, and to train research teams in this area which is one of the main purposes of this international symposium. With the publication of the papers presented at this symposium and the records of group discussions, it is expected to highlight the main scheme and bottlenecks of researches

on insect-mediated pathogens and insect vectors which can be a vital reference for the authorities in agriculture sector and researchers to develop related research plans. The scholars who participate in this international academic symposium are welcome to maintain cooperation with researchers in Taiwan. Together, the fundamental knowledge of insect-mediated pathogens and insect vectors would be built, and the technology that can fulfill the needs of the industry will be created in the future.

ACKNOWLEDGMENTS

This seminar was supported by the grants (102AS-4.1.1-CI-C1 and 102AS-4.1.1-ST-a3). As the project director, the first author is grateful to the agencies for the financial supports.

LITERATURE CITED

1. Almeida, R. P. P., Blua, M. J., Lopes, J. R. S., and Purcell, A. H.. 2005. Vector transmission of Xylella fastidiosa: Applying fundamental knowledge to generate disease management strategies. Ann. Entomol. Soc. Am. 98:775-786.

2. Berisha, B., Chen, Y. D., Zhang, G. Y., Xu, B. Y., and Chen, T. A. 1998. Isolation of Pierce’s disease bacteria from grapevines in Europe. Eur. J. Plant Pathol.

104:427-433.

3. Chang, C. J., Shih, H. T., Su, C. C., and Jan, F. J. 2012. Diseases of important crops, a review of the causal fastidious prokaryotes and their insect vectors. Plant Pathol. Bull.

21:1-10.

4. Chu, Y. J. 2001. Pierce’s disease and control techniques. Yantai Fruits 2001 (4):11-12.

5. Chu, Y. J. 2002. Pierce’s disease and control techniques. Hebei Fruits 2002 (1):44-45.

6. Freitag, J. H. 1951. Host range of Pierce’s disease virus of grapes as determined by insect transmission. Phytopathology 41:920-934.

7. Hopkins, D. L., and Adlerz, W. C. 1988. Natural hosts of Xylella fastidiosa in Florida.

Plant Dis. 72:429-31.

8. Hopkins, D. L. 1989. Xylella fastidiosa: xylem-limited bacterial pathogen of plants.

Ann. Rev. Phytopathol. 27:271-290.

9. Janse, J. D., and Obradovic, A. 2010. Xylella fastidiosa: its biology, diagnosis, control and risks. J. Plant Pathol. 92 (Supplement 1):S1. 35-48.

10. Metcalf, Z. P. 1965. General catalogue of the Homoptera. Fasc. VI. Cicadelloidea.

Part 1. Tettigellidae. USDA-ARS, Washington. 730 pp.

11. Oman PW. 1949. The Nearctic leafhoppers (Homoptera: Cicadellidae). a generic classification and check list. Wash. Ent. Soc. Mem. 3:1-253.

12. Purcell, A. H. 1982. Insect vectors relationships with prokaryotic plant pathogens.

Annu. Rev. Phytopathol. 20:397-417.

13. Purcell, A. H. 1997. Xylella fastidiosa, a regional problem or global threat? J. Plant Pathol. 79:99-105.

14. Purcell, A. H., and Hopkins, D. L. 1996. Fastidious xylem-limited bacterial plant pathogens. Annu. Rev. Phytopathol. 34:131-151.

15. Raju, B. C., Goheen, A. C., Teliz, D., and Nyland, G. 1980. Pierce’s disease of grapevines in Mexico. Plant Dis. 64:280-282.

16. Redak, R. A., Purcell, A. H., Lopes, J. R. S., Blua, M. J., Mizell III, R. F., and Andersen, P. C. 2004. The biology of xylem fluid-feeding insect vectors of Xylella fastidiosa and their relation to disease epidemiology. Annu. Rev. Entomol.

49:243-270.

17. Severin, H. H. P. 1950. Spittle-insect vectors of Pierce’s disease virus. II. Life history and virus transmission. Hilgardia 19:357-382.

18. Shih, H. T., Dietrich, C. H., and Yang, J. T.. 2004. Use of vineyards as habitats by leafhoppers (Insecta: Hemiptera: Cicadelloidea) in central Taiwan. Plant Prot. Bull.

45:405- 406.

19. Shih, H. T., Su, C. C., Feng, C. Y., Fanjiang, C. C., Hung, W. F., and Hung, L. Y.

2009. Studies on the morphology, ecology, and host range for Kolla paulula (Walker, 1858) (Hemiptera: Membracoidea: Cicadellidae: Cicadellinae). Formosan Entomol. 29(4):353 (in Chinese).

20. Shih, H. T., Lee, C. Y., Wen, Y. D., Su, C. C., Chang, S. C., Chang, C. J., Tuan, S. J., and Feng, C. Y. 2011. Advance and application prospect in an integrated management of the vectors of plant pathogenic prokaryotes. p. 107-122. In: Shih and Chang [eds.], Proceedings of the symposium on integrated management technology of insect vectors and insect-borne diseases. Special Publication of TARI No. 152. Taiwan Agricultural Research Institute, Bureau of Animal and Plant Health Inspection and Quarantine. 222 pp. (in Chinese with English abstract)

21. Su, C. C., Shih, H. T., Lin, Y. S., Su, W. Y., and Kao, C. W. 2011. Current status of Pierce’s disease of grape and its vector in Taiwan. p. 25-50. In: Shih and Chang

[eds.], Proceedings of the symposium on integrated management technology of insect vectors and insect-borne diseases. Special Publication of TARI No. 152.

Taiwan Agricultural Research Institute, Bureau of Animal and Plant Health Inspection and Quarantine. 222 pp. (in Chinese with English abstract)

22. Su, C. C., Chang, C. J., Chang, C. M., Shih, H. T., Tzeng, K. C., Jan, F. J., Kao, C.

W., and Deng, W. L. 2013. Pierce's disease of grapes in Taiwan: Isolation, cultivation, and pathogenicity of Xylella fastidiosa. J. Phytopathol. 161:389-396.

23. Yang, M. F., Deitz, L. L., and Li, Z. Z. 2005. A new genus and two new species of Cicadellinae from China (Hemiptera: Cicadellidae), with a key to the Chinese genera of Cicadellinae. J. New York Entomol. Soc. 113:77-83.

24. Young, D. A. 1986. Taxonomic Study of the Cicadellinae (Homoptera: Cicadellidae) Part 3. Old World Cicadellini. North Carolina Agric. Res. Ser. Tech. Bull.

281:1-639.

Table 1. From January, 2009 to August, 2011, the monthly average temperature and total rainfall, and the populations of Kolla paulula at Bai-mao-tai area of Hsinshe District in Taichung City 1 2 3 4 5 6 7 8 9 10 11 12 2009 The period of peak occurrence (every ten days)

Monthly average temperature 12.16 18.59 17.71 19.99 23.25 25.48 26.14 26.32 25.62 23.7519.82 15.23 Monthly total rainfall 0 mm 15 mm 189.7 mm385.6 mm22 mm 93.8 mm 111 mm996.5 mm27.5 mm13.5 mm23.5 mm 22.5 mm 2010 The period of peak occurrence (every ten days)

Monthly average temperature 14.95 16.86 19.34 20.73 24.68 25.22 26.84 26.28 25.68 23.43 18.66 15.46 Monthly total rainfall 47.9 mm 155.8 mm32.5 mm171.5 mm26 mm 795 mm 392 mm376.5 mm228.8 mm6.5 mm22.9 mm 37.3 mm 2011 The period of peak occurrence (every ten days)

Monthly average temperature - 15.04 15.54 20.71 23.98 27 26.56 27.01 - - - - Monthly total rainfall - 33.5 mm52.5 mm2.5 mm 157.5 mm185 mm 354.2 mm250 mm- - - - Remarks: represents the first peak of the population occurred every year; represents the second peak of the population occurred every year; represents the third peak of the population occurred every year.

Understanding Bacterial Virulence Genes and Mechanisms of