11. General conclusions
11.3 Part 3: Using layer by layer gold nanoparticles hybridization method to
sensing system
Every year, a lot of cases of DHF/DSS occur in epidemic areas and resulted in thousands people deaths. Since there is no effective vaccine to prevent these cases, early diagnosis in the course of the disease may allow for more rapid treatment of cases progressing to DHF/DSS.
In our study, we showed that a sensitive QCM DNA sensor can diagnosed dengue virus in low titer, and combined AuNPs in system as “mass enhancers” for enhancing signal. In previous report, AuNPs-probe always used as enhancers to improve the DNA-sensor sensitivity, and used only one layer AuNPs-probe in their system. In this study, 2nd layer AuNPs-probe are applied not only as enhancer but as played captor. In circulation-system, AuNPs circulate in system and hybridized with surplus target, the target conjugating with AuNPs-probe would move near the QCM chip easily, and QCM system would be more efficient for detection. Using AuNPs-probe effectively amplified the signals in frequency shifts due to their relatively large mass compared to DNA targets, and combined layer by layer AuNPs can step up the surface area of detection and enhance more frequency change in a short time. The circulation system is different from open system, efficiency of different sizes of AuNPs are not similar as Liu et al (2004). In four sizes of AuNPs, 13 nm
AuNPs-probe not only on the 1st layer but also on the 2nd layer have the best efficiency to enhance the mass.
Further, layer by layer AuNPs not only enhanced mass, but also increased some surface area, the dendritic surface can interact with surplus target and AuNPs (conjugated with DENV-P1 or DENV-P2) more efficient than flat surface or one layer AuNPs (frequency change within 30 min), specifically when detected low concentration PCR product.
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