Third World Congress of the International Academy of Oral Oncology
(IAOO)
ABSTRACT SUBMISSION
Title: The Evaluation of Hinokitiol in Oral Squamous Cell Carcinoma Prevention
Abstract No. 0327
Title The Evaluation of Hinokitiol in Oral Squamous Cell Carcinoma Prevention
Abstract Introduction:
Hinokitiol is a constituent of the woody oils isolated from Cupressaceae heartwood. Hinokitiol had been found to be act as antibacterial, antifungal, and antitumor agents. There are a few of studies about hinokitiol with oral cancer. It is interesting that wether hinokitiol has anti-oral cancer potential and how dose hinokitiol to activate the critical anti-oral cancer mechanism.
Methods:
The cytotoxicity of Hinokitiol was evaluated by MTT test including 5 oral squmaous cell carcinoma cell lines (OSCC cell lines: HSC3, OC3, OECM-1, SAS, and SCC4), 5 normal human oral keratinocyte (NHOK from different donors), and 5 normal human oral fibroblast (NHOF from different donors). Cell cycle was analyzed by flow cytometry. The malignant phenotypes including cell colony formation, migration, invasion, were using transwell, and anchorage independent ability was using soft agar assay.
Results:
Hinokitiol was more cytotoxic to 5 OSCC cell lines than 5 NHOKs, and 5 NHOFs. HSC3 was the most sensitive OSCC cell line in hinokitiol treatment. The cell cycle of most cell lines in the study was arrested at G1 phase after incubating with IC50 hinokitiol concentration 24 hr. Low dosages hinokitiol (no significant cytotoxicity in MTT test) inhibited HSC3 cell colony formation, migration, and invasion ability, and SCC4 anchorage independent growth.
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Affiliations (1) Department of Dental Hygiene, College of Health Care, China Medical University, Taichung,, n/a, Taiwan
(2) Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei,, n/a, Taiwan
(3) Department of Nutrition and Health Sciences, School of Health Care Management, Kainan University, Taoyuan,, n/a, Taiwan
Authors T-M. Shieh (1) (1) Presenting Y-H. Shih (2) (2)
S-C. Lin (2) (2) S-M. Hsia (3) (3)
Presenter
email bojukimo@yahoo.com.tw Categories Molecular biology and genetics
Keyword1 cytotoxicity
Keyword2 migration
Keyword3 invasion
Keyword4 anchorage independent
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Oxford Abstracts: IAOO 2011
2011/2/13
https://elsevier.conference-services.net/abstracts.asp?sessionID={B0F46C29-78CD-4A...
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