17" Conference on
B
iochemical Engineering
June,25-26.2012 國 立高雄師範大學
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E. coli Nissle reduced cardiac dysfunction by decreasing apoptotic
pathway and enhancing survival pathway activities in diabetic rats
4 Departm
PinNing Wang1#, ShengChuan Lin2, ChangYu Chang2 -Chih-Yang Huang3, Chung-Jen Chiang2*, Yun-Peng Chao4*
department ofInformation Engineering and Computer Science, Feng Chia
University, Taichung 407,Taiwan.
2Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan. [email protected]
3Graduate Institute of Basic Medical Science, China Medical University, Taichung 404, Taiwan.
Department of Chemical Engineering, Feng Chia University, Taichung 407, TaiwaaL
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c:99-EC-17-A-HW51-156
Previous studies found that E.coli Nissle is a typical E.coM steam and
non-pathogenic. It could not produce any enterotoxin or cytotoxin and no pathogenic
adhesion molecule has been found. The E. coli Nissle has been shown its beneficial effects on human gastrointestinal tract. However, there are no reports so far to reveal the effects of E.coli Nissle on cardiovascular disease. To investigate whether E. coli Nissle has protective effects against diabetic mellitus (DM)-induced heart disease, our animal experiment was designed as follows: sixty 5-week-old male wistar rats were divided into three groups: normal control group, diabetes DM group [55mg / kgw Streptozotocin (STZ) induced DM, for Intraperitoneal injection], and DM animals with lactic acid bacteria E.coli Nissle (109cfu/ rat / day, treatmentfor 4 weeks).
Our data show that(l) increased blood sugar(418 126.36 V.S control 91.22 8.06) and loss body weight (268 42.07 V.S control 342 23.93) ; (2) the pro-apoptotic proteins, including Fas, FADD, caspase-8, activated-Bak, Bax, Bad, caspase-9, caspase-3 and released Cytochrome C were increased significantly in DM groups. After treatment with E.coli Nissle, blood sugar of DM rats returned to normal range and improved body weight markedly. Meanwhile, western blot analysis show that both death receptor-dependent and mitochondrial-dependent related pro-apoptotic proteins were decreased markedly compare to the untreated group. Furthermore, TUNEL assay show significantreduction of apoptosis levelin left ventriculartissue in E.coli Nissle treatment group. These finding provide the evidence show that in addition to intestinal health, E.coli Nissle may be the potential to cure DM-induce heart disease.
Keyword: E. coli Nissle - diabetes * heart health 255
