Association of X-ray Repair Cross-complementing-6 Genotypes with Childhood Leukemia
JEN-SHENG PEI 1, YI-MIN LEE 2, HSUEH-HSIA LO 2, YUAN-NIAN HSU 3, SONG-SHEI LIN 4,* and DA-TIAN BAU 5,6,*
1Department of Pediatrics, Taoyuan General Hospital, Ministry of Health and Welfare,
Taoyuan, Taiwan, ROC;
2Department of Medical Laboratory Science and Biotechnology, Central-Taiwan University
of Science and Technology, Taichung, Taiwan, ROC;
3Department of Family Medicine, Taoyuan General Hospital, Ministry of Health and
Welfare, Taoyuan, Taiwan, ROC;
4Department of Medical Imaging and Radiological Sciences, Central-Taiwan University of
Science and Technology, Taichung, Taiwan, ROC;
5Graduate Institute of Clinical Medical Science, China Medical University, Taichung,
Taiwan, ROC;
6Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung,
Taiwan, ROC. * These Authors contributed equally to this work
Correspondence to: Song-Shei Lin and Da-Tian Bau, Terry Fox Cancer Research Laboratory, China Medical University and Hospital, Taichung, Taiwan 40421, Republic of
China, Tel: +886 422052121 Ext. 1523, email: [email protected]; [email protected]
Running title: Pei et al: XRCC6 in Childhood Leukemia
Key Words: XRCC6/Ku70, non-homologous end-joining, childhood leukemia, polymorphisms, Genotyping.
Abstract.
Background: The Non-homologous end-joining repair gene XRCC6/Ku70 plays an important role in the repair of DNA double-strand breaks (DSBs), and has been found to be involved in the carcinogenesis of many types of cancers including oral, prostate, breast and bladder cancer. However, the contribution of XRCC6 to childhood leukemia has yet to be studied. In the present study, we investigated the association of XRCC6 genotypes with the risk of childhood leukemia. Materials and Methods: Two hundred and sixty-six patients with childhood leukemia and equal number of age-matched healthy controls recruited in Central Taiwan, were genotyped investigating these polymorphisms’ association with childhood leukemia. Results: As for XRCC6 promoter T-991C, patients carrying the TC genotype had significantly increased risk of childhood leukemia compared with the TT wild-type genotype [odds ratio (OR)= 2.30, 95% confidence interval (CI)= 1.38-3.84, p=0.0047]. Meanwhile, the genotypes of XRCC6 promoter C-57G, A-31G and intron3 were not statistically associated with childhood leukemia risk. Conclusion: Our findings suggest that XRCC6 genotype could serve as a predictor of childhood leukemia risk and XRCC6 could serve as a target for personalized medicine and therapy.Childhood leukemia is the most common childhood cancer worldwide and a severe condition affecting all societies around the globe. The initiation etiology and genomic-contributing factors of leukemia are still largely unknown, both in adult and childhood leukemia.Most possibly, ionizing radiation, chemicals (such as benzene), drugs (such as alkylating agents), genetic, single-gene disorders (such as ataxia telangiectasia, neurofibromatosis, Blackfan-Diamond syndrome), and chromosome abnormalities are implicated in the appearance of childhood leukemia, whereas solid evidence are still lacking. It is commonly recognized that single environmental or genetic factors can only ambiguously explain a small part of subjects who develop childhood leukemia.. Cell response to various genetic injury and its ability to maintain genomic stability by a cooperative DNA repair network are essential in preventing tumor initiation and progression. Accumulated mutations and/or defects in the DNA repairing system are essential for tumorigenesis. It is, therefore, logical to suspect that some genetic variants of these DNA repair genes, such as X-ray cross-complementing group-6 (XRCC6), might contribute to childhood leukemia pathogenesis.
The human genome integrity is maintained by the human DNA repair network, in which accumulated mutations and/or defects are thought to be essential for carcinogenesis (1, 2). For this reason, we hypothesized that the loss or lower of function of DNA repair capacity via genomic variation might have a significant influence on childhood leukemia carcinogenesis. In humans, genetic variations on the predominant non-homologous end-joining (NHEJ), together with those on the alternative homologous recombination DNA double strand break repair subpathway, have been postulated as an important contributor to the etiology of cancer (3). In recent years, several proteins involved in the NHEJ pathway have been identified, including ligase IV, XRCC4, XRCC6 (Ku70), XRCC5 (Ku80), DNA-PKcs, Artemis and XLF (4, 5).
Also, Inappropriate NHEJ can lead to translocations and telomere fusion, hallmarks of tumor cells (6). As for NHEJ, some genetic polymorphisms were reported to influence DNA repair capacity and confer predisposition to several types of cancer, including skin (7), breast (8-10), bladder (11, 12), lung (13) and oral cancers (14, 15). However, there is no information regarding childhood leukemia and NHEJ gene polymorphisms. Recently, epidemiological studies have investigated the association between the XRCC6 polymorphism and the risk for different types of cancer, including gastric cancer (16), oral cancer (14), breast cancer (17), lung cancer (13) and renal cell carcinoma (18). We hypothesized that differential XRCC6 genotypes may also contribute to childhood leukemia susceptibility. To test this hypothesis, our present study was designed to investigate the association of XRCC6 genotypes with risk of childhood leukemia in our case-control study in a population from central Taiwan. To the best our knowledge, this is the first study to evaluate the XRCC6 polymorphisms, in both a Taiwanese childhood leukemia population and globally.
Materials and Methods
Study population and sample collection. Two hundred and sixty-six patients diagnosed with childhood leukemia (a population aged <18 years ) were recruited at the Pediatric Departments at the China Medical University Hospital and National Taiwan University Hospital, Taiwan, Republic of China in 2005-2009. Each patient and non-cancer healthy person (matched with gender and age after initial random sampling from the Health Examination Cohort of the two hospitals) completed a self-administered questionnaire and provided with their peripheral blood samples.
Genotyping assays. Genomic DNA was prepared from peripheral blood leucocytes using a QIAamp Blood Mini Kit (Blossom, Taipei, Taiwan) and processed further according to our previous articles (19-22). The primers used for XRCC6 promoter C-991T were: forward AACTCATGGACCCACGGTTGTGA-3’, and reverse CAACTTAAATACAGGAATGTCTTG-3’; for promoter G-57C were: forward
5’-AAACTTCAGACCACTCTCTTCT-3’, and reverse
AAGCCGCTGCCGGGTGCCCGA-3’; for promoter G-31A were: forward 5’-TACAGTCCTGACGTAGAAG-3’, and reverse AAGCGACCAACTTGGACAGA-3’; for intron3 were forward GTATACTTACTGCATTCTGG-3’, and reverse 5’-CATAAGTGCTCAGTACCTAT-3’. The following cycling conditions were performed: one cycle at 94°C for 5 min; 35 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s; and a final extension at 72°C for 10 min. The PCR cycling conditions performed were: one cycle at 94oC for 5 min; 35 cycles of 94oC for 30 s, 55oC for 30 s, and 72oC for 30 s; and a final extension step at 72oC for 10 min.
RFLP conditions. As for the XRCC6 promoter C-991T, the resultant 301-bp PCR product was mixed with 2 U Dpn II. The restriction site was located at -991 with a C/T polymorphism, and the C form PCR products could be further digested while the T form could not. Two fragments of 101 bp and 200 bp were present if the product was of the digestible C form. The reaction was incubated for 2 h at 37°C. Then, 10 l of product was loaded into a 3% agarose gel containing ethidium bromide for electrophoresis. The polymorphism was categorized as either (a) C/C homozygote (digested), (b) T/T homozygote (undigested), or (c) C/T heterozygote. As for the XRCC6 promoter G-57C, the resultant 298 bp PCR products were mixed with 2 U Hae II. The restriction site was located at -57 with a C/G polymorphism, and the G form PCR products could be further digested while the C form could not. Two fractions 103 and 195 bp were present if the
product was digestible G form. The reaction was incubated for 2 h at 37°C. Then, 10 l of product was loaded into a 3% agarose gel containing ethidium bromide for electrophoresis. The polymorphism was categorized as either (a) G/G homozygote (digested), (b) C/C homozygote (undigested), or (c) C/G heterozygote. As for the XRCC6 promoter A-31G, the resultant 226 bp PCR products were mixed with 2 U Mnl I. The restriction site was located at -31 with a A/G polymorphism, and the A form PCR products could be further digested while the G form could not. Two fractions 80 and 146 bp were present if the product was digestible A form. The reaction was incubated for 2 h at 37°C. Then, 10 l of product were loaded into a 3% agarose gel containing ethidium bromide for electrophoresis. The polymorphism was categorized as either (a) A/A homozygote (digested), (b) G/G homozygote (undigested), or (c) A/G heterozygote. As for the XRCC6 promoter intron3, the resultant 160-bp PCR products were mixed with 2 U Msc I. The restriction site was located at intron3 with a TGG/CCA polymorphism, and the CCA form PCR products could be further digested while the TGG form could not. Two fractions of 46 and 114 bp were present if the product was of the digestible CCA form. The reaction was incubated for 2 h at 37°C. Then, 10 l of product was loaded into a 3% agarose gel containing ethidium bromide for electrophoresis. The polymorphism was categorized as either (a) CCA/CCA homozygote (digested), (b) TGG/TGG homozygote (undigested), or (c) CCA/TGG heterozygote.
Statistical analyses. Matches with all DNA data of polymorphisms (case/control=266/266) were selected for the final analysis. To ensure that the controls used were representative of the general population and to exclude the possibility of genotyping error, the deviation of the genotype frequencies of XRCC6 SNPs in the control subjects from those expected under the Hardy-Weinberg equilibrium was
assessed using the goodness-of-fit test. Pearson’s Chi-square test or Fisher’s exact test (when the expected number in any cell was less than five) was used to compare the distribution of the XRCC6 genotypes between cases and controls. The associations between the XRCC6 polymorphisms and childhood leukemia risk were estimated by computing odds ratios (ORs) and their 95% confidence intervals (CIs) from unconditional logistic regression analysis with the adjustment for possible confounders. p-Values less than 0.05 was considered statistically significant, and all statistical tests were two-sided.
Results
Genotypic distributions of the XRCC6 promoter T-991C among cases and controls are presented in Table I. The ORs for patients carrying TC and CC genotypes were 2.30 (95%CI=1.38-3.84) and 1.69 (95%CI=0.28-10.22) respectively, compared to those carrying TT wild-type genotype (Table I). The p-value for trend was significant at p=0.0047. In the dominant (TC-plus-CC-versus-TT) model, the association between XRCC6 promoter T-991C polymorphism and the risk for childhood leukemia was still statistically significant (OR=2.25, 95%CI=1.37-3.71). However, as for the XRCC6 promoter C-57G (Table II), A-31G (Table III), and intron3 polymorphisms (Table IV), there were no difference between childhood leukemia and control groups in the genotype frequency distribution.
Discussion
In the present study, the associations of XRCC6 genotypes and the risk of childhood leukemia were investigated in a Taiwanese population. After genotyping of the four SNPs, it was shown that individuals carrying the TC and CC genotypes were of higher risk of childhood leukemia compared with those carrying TT genotype at XRCC6 T-991C. To the best of our knowledge, this is the first study investigating the role of XRCC6 in childhood leukemia with positively associated findings.
XRCC6 may work together with XRCC5 as a hetero-dimer, while may work independently of XRCC5 as well (23). Proteomic defects in XRCC6 may cause not only lower DSB repair capacity, but also growth retardation, ionizing radiation hypersensitivity, and severe combination immune deficiency due to severely-impaired variable division joining recombination capacity (24). From the genomic viewpoint, small genomic variations in XRCC6 such as polymorphisms might escape the cell-cycle checking point, and also lead to sub-optimal DNA repair capacity which would accumulate DNA damage step by step triggering childhood leukemia tumorigenesis (8, 9, 25).
Among the various types of cancer, recently there were some epidemiological studies investigating the association between XRCC6 T-991C polymorphism and its risk for gastric (16), oral (14), breast cancer (17), hepatocellular carcinoma (26) and cancer-like pterygium (27). Our data together with these of previous reports could be interpreted as pointing towards the concept that DNA repair gene XRCC6 may play a common role in cancer initiation in the carcinogenesis of leukemia, in addition to its role in solid tumors.
The XRCC6 T-991C genotypic variation mapped to the promoter region of XRCC6 does not directly result in amino-acid coding alteration. It is possible that carrier may
have different expression levels or stability of the XRCC6 protein, similar to the case of XRCC4 (15, 28). Although we could not provide evidence from functional experiments examining whether the XRCC6 T-991C SNP could influence downstream mRNA and protein levels, but we have found positive correlation among hepatocellular carcinoma patients with various XRCC6 T-991C genotypes. From the results of real-time quantitative RT-PCR, we have found that the tissues of C allele indeed had a lower expression level of the XRCC6 mRNA than those of T at XRCC6 T-991C. The obtained results on the protein levels supported this hypothesis as well (26). The T allele might increase the expression levels of XRCC6 mRNA, which may lead to increased expression of XRCC6 protein and elevated DSB repair capacity, producing protective effects on normal tissues.
The present study suffers certain limitations. Firstly, the sample size is moderate, fact that may restrict the reliability and feasibility of stratification and interaction analyses. Secondly, it is not easy to separate the samples into leukemia subtypes. Thirdly, the insufficient clinical and behavioral information, such as patient breeding status, daily diet habits, limited our capacity of performing related risk factor analyses or genotype-lifestyle interaction analyses.
In conclusion, our current study indicated that the functional XRCC6 T-991C polymorphism is associated with childhood leukemia susceptibility in a Taiwanese population..Whether this novel functional XRCC6 polymorphism leads to differential XRCC6 mRNA and protein expression levels will be examined in the near future.
Acknowledgements
This work was supported by Grants PTH10236 from Central-Taiwan University of Science and Technology and Taoyuan General Hospital, Ministry of Health and
References
1. Vogelstein B, Alberts B and Shine K: Genetics. Please don't call it cloning! Science 295: 1237, 2002.
2. Miller KL, Karagas MR, Kraft P, Hunter DJ, Catalano PJ, Byler SH and Nelson HH: XPA, haplotypes, and risk of basal and squamous cell carcinoma. Carcinogenesis 27: 1670-1675, 2006.
3. Goode EL, Ulrich CM and Potter JD: Polymorphisms in DNA repair genes and associations with cancer risk. Cancer Epidemiol Biomarkers Prev 11: 1513-1530, 2002.
4. Jackson SP: Sensing and repairing DNA double-strand breaks. Carcinogenesis 23: 687-696, 2002.
5. Lieber MR: The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway. Annu Rev Biochem 79: 181-211, 2010.
6. Espejel S, Franco S, Rodriguez-Perales S, Bouffler SD, Cigudosa JC and Blasco MA: Mammalian Ku86 mediates chromosomal fusions and apoptosis caused by critically short telomeres. Embo J 21: 2207-2219, 2002.
7. Han J, Colditz GA, Samson LD and Hunter DJ: Polymorphisms in DNA double-strand break repair genes and skin cancer risk. Cancer Res 64: 3009-3013, 2004. 8. Bau DT, Fu YP, Chen ST, Cheng TC, Yu JC, Wu PE and Shen CY: Breast
cancer risk and the DNA double-strand break end-joining capacity of nonhomologous end-joining genes are affected by BRCA1. Cancer Res 64: 5013-5019, 2004.
9. Bau DT, Mau YC, Ding SL, Wu PE and Shen CY: DNA double-strand break repair capacity and risk of breast cancer. Carcinogenesis 28: 1726-1730, 2007. 10. Chiu CF, Wang HC, Wang CH, Wang CL, Lin CC, Shen CY, Chiang SY and
Bau DT: A new single nucleotide polymorphism in XRCC4 gene is associated with breast cancer susceptibility in Taiwanese patients. Anticancer Res 28: 267-270, 2008.
11. Chang CH, Chang CL, Tsai CW, Wu HC, Chiu CF, Wang RF, Liu CS, Lin CC and Bau DT: Significant association of an XRCC4 single nucleotide polymorphism with bladder cancer susceptibility in Taiwan. Anticancer Res 29: 1777-1782, 2009.
12. Chang CH, Wang RF, Tsai RY, Wu HC, Wang CH, Tsai CW, Chang CL, Tsou YA, Liu CS and Bau DT: Significant association of XPD codon 312 single nucleotide polymorphism with bladder cancer susceptibility in Taiwan. Anticancer Res 29: 3903-3907, 2009.
13. Hsia TC, Liu CJ, Chu CC, Hang LW, Chang WS, Tsai CW, Wu CI, Lien CS, Liao WL, Ho CY and Bau DT: Association of DNA double-strand break gene XRCC6 genotypes and lung cancer in Taiwan. Anticancer Res 32: 1015-1020, 2012.
14. Bau DT, Tseng HC, Wang CH, Chiu CF, Hua CH, Wu CN, Liang SY, Wang CL, Tsai CW and Tsai MH: Oral cancer and genetic polymorphism of DNA double strand break gene Ku70 in Taiwan. Oral Oncol 44: 1047-1051, 2008. 15. Chiu CF, Tsai MH, Tseng HC, Wang CL, Wang CH, Wu CN, Lin CC and Bau
DT: A novel single nucleotide polymorphism in XRCC4 gene is associated with oral cancer susceptibility in Taiwanese patients. Oral Oncol 44: 898-902, 2008. 16. Yang MD, Wang HC, Chang WS, Tsai CW and Bau DT: Genetic
polymorphisms of DNA double strand break gene Ku70 and gastric cancer in Taiwan. BMC Cancer 11: 174, 2010.
17. Fu YP, Yu JC, Cheng TC, Lou MA, Hsu GC, Wu CY, Chen ST, Wu HS, Wu PE and Shen CY: Breast cancer risk associated with genotypic polymorphism of the
nonhomologous end-joining genes: a multigenic study on cancer susceptibility. Cancer Res 63: 2440-2446, 2003.
18. Chang WS, Ke HL, Tsai CW, Lien CS, Liao WL, Lin HH, Lee MH, Wu HC, Chang CH, Chen CC, Lee HZ and Bau DT: The role of XRCC6 T-991C functional polymorphism in renal cell carcinoma. Anticancer Res 32: 3855-3860, 2012.
19. Yang MD, Hsu YM, Kuo YS, Chen HS, Chang CL, Wu CN, Chang CH, Liao YM, Wang HC, Wang MF and Bau DT: Significant association of Ku80 single nucleotide polymorphisms with colorectal cancer susceptibility in Central Taiwan. Anticancer Res 29: 2239-2242, 2009.
20. Chang CH, Chiu CF, Liang SY, Wu HC, Chang CL, Tsai CW, Wang HC, Lee HZ and Bau DT: Significant association of Ku80 single nucleotide polymorphisms with bladder cancer susceptibility in Taiwan. Anticancer Res 29: 1275-1279, 2009.
21. Hsu NY, Wang HC, Wang CH, Chiu CF, Tseng HC, Liang SY, Tsai CW, Lin CC and Bau DT: Lung cancer susceptibility and genetic polymorphisms of Exo1 gene in Taiwan. Anticancer Res 29: 725-730, 2009.
22. Hsu CF, Tseng HC, Chiu CF, Liang SY, Tsai CW, Tsai MH and Bau DT: Association between DNA double strand break gene Ku80 polymorphisms and oral cancer susceptibility. Oral Oncol 45: 789-793, 2009.
23. Wang J, Dong X, Myung K, Hendrickson EA and Reeves WH: Identification of two domains of the p70 Ku protein mediating dimerization with p80 and DNA binding. J Biol Chem 273: 842-848, 1998.
24. Khanna KK and Jackson SP: DNA double-strand breaks: signaling, repair and the cancer connection. Nat Genet 27: 247-254, 2001.
end-joining. Cancer Lett 240: 1-8, 2006.
26. Hsu CM, Yang MD, Chang WS, Jeng LB, Lee MH, Lu MC, Chang SC, Tsai CW, Tsai Y, Tsai FJ, Bau DT: The contribution of XRCC6/Ku70 to hepatocellular carcinoma in Taiwan. Anticancer Res 33: 529-535, 2013.
27. Tsai YY, Bau DT, Chiang CC, Cheng YW, Tseng SH and Tsai FJ: Pterygium and genetic polymorphism of DNA double strand break repair gene Ku70. Mol Vis 13: 1436-1440, 2007.
28. Bau DT, Yang MD, Tsou YA, Lin SS, Wu CN, Hsieh HH, Wang RF, Tsai CW, Chang WS, Hsieh HM, Sun SS and Tsai RY: Colorectal cancer and genetic polymorphism of DNA double-strand break repair gene XRCC4 in Taiwan. Anticancer Res 30: 2727-2730, 2010.
Table I. Genotypic and allelic frequencies of XRCC6 promoter T-991C among the childhood leukemia patients and healthy controls.
Cases n=266 Controls n=266 p-Value OR (95%CI)
Genotype
0.0047 T/T 212 (79.7%) 239 (89.8%) 1.00 (ref) T/C 51 (19.2%) 25 (9.4%) 2.30 (1.38-3.84) C/C 3 (1.1%) 2 (0.8%) 1.69 (0.28-10.22) T/C or C/C 54 (20.3%) 27 (10.2%) 2.25 (1.37-3.71)Allele
0.0016 T 475 (89.3%) 503 (94.5%) 1.00 (ref) C 57 (10.7%) 29 (5.5%) 2.08 (1.31-3.31)Table II. Genotypic and allelic frequencies of XRCC6 promoter C-57G among in childhood leukemia patients and healthy controls.
Cases n=266 Controls n=266 p-Value OR (95% CI)
Genotype
C/C 194 (72.9%) 188 (70.7%) 0.8259 1.00 (ref) C/G 68 (25.6%) 73 (27.4%) 0.90 (0.61-1.33) G/G 4 (1.5%) 5 (1.9%) 0.78 (0.21-2.93) C/G or G/G 72 (27.1%) 78 (29.3%) 0.89 (0.61-1.31)Allele
C 456 (85.7%) 449 (84.4%) 0.5472 1.00 (ref) G 76 (14.3%) 83 (15.6%) 0.90 (0.64-1.26)Table III. Genotypic and allelic frequencies of XRCC6 promoter A-31G among in childhood leukemia patients and healthy controls.
Cases n=266 Controls n=266 p-Value OR (95% CI)
Genotype
A/A 228 (85.7%) 222 (83.5%) 0.6236 1.00 (ref) A/G 30 (11.3%) 32 (12.0%) 0.91 (0.54-1.55) G/G 8 (3.0%) 12 (4.5%) 0.65 (0.26-1.62) A/G or G/G 38 (14.3%) 44 (16.5%) 0.84 (0.52-1.35)Allele
A 486 (91.4%) 476 (89.5%) 0.2977 1.00 (ref) G 46 (8.6%) 56 (10.5%) 0.80 (0.53-1.21)Table IV. Genotypic and allelic frequencies of XRCC6 intron3 in childhood leukemia patients and healthy controls.
Cases n=266 Controls n=266 p-Value OR (95% CI)
