Immunohistochemical localization of
manganese superoxide dismutase in the
rat cochlea
賴銘堂
Lai MT;Ohmichi T;Egusa K;Okada S and Masuda Y
摘要
Abstract
There has been recent increasing interest in the involvement of superoxide radicals (O2 –) and their scavenging enzymes, the superoxide dismutases, in the pathophysiology of certain diseases. Since mitochondria are significant intracellular sources of O2 – and important targets of oxidant injury, determining the intracochlear localization of mitochondrial O2 – scavenging enzyme may provide important insight into the
pathogenesis of injury due to cochlear oxidants. In order to locate the mitochondrial O2 – scavenging enzyme, manganese superoxide dismutase (MnSOD), the authors used a modified immunoglobulin peroxidase bridge sequence method to detect MnSOD in paraffin-embedded, formalin-fixed rat cochleas. Site-specific immunolocalization of MnSOD could be demonstrated in the cochlear labyrinth, suggesting that the generation of intracochlear O2 – was possibly implicated in the metabolically active sites and sites rich in vascularity. This study also provided a useful probe for detecting MnSOD
immunohistochemically from ethylenediamine tetraacetic acid-treated materials without requiring an antigen retrieval procedure.
