甲基類固醇與紅血球生成素對脊髓損傷的影響:生物晶片資料分析
The influence of Methylprednisolone and Erythropoietin to spinal cord injury:microarray data analysis
中文摘要
在藥物 MP 與 EPO 治療對於脊髓損傷的研究中，顯示藥物 MP 有極大的副作用，
雖然對於急性期的脊髓損傷治療有幫助，但長期使用會造成很多後遺症；藥物 EPO 對於脊髓損傷恢復已有動物實驗證實比藥物 MP 更好並且它的副作用較 少。我們發現脊髓損傷的相關機制仍然有許多不是很清楚，臨床上真正有助於恢 復的藥物也尚未找到。故我們希望能全面性地了解脊髓損傷的基本反應機制、分 別再進一步地探討藥物 MP 與 EPO 對脊髓損傷的反應機制。我們將設計不同的 時間點，在時間序列上檢視基因表現量的變化，希望可以區分出藥物 MP 與 EPO 對於脊髓損傷反應基因的類型，綜觀這些反應基因群，我們將以生物網絡的方式 呈現，提供對於脊髓損傷的反應機制更深入的剖析。
英文摘要
Treatment of spinal cord injury (SCI) has been a frustrating endeavor in neurological medicine because SCI causes a series of devastating damage.
The current treatment of SCI is restricted in the high-dose glucocorticoid drug. The anti-inflammatory therapy, Methylprednisolone (MP), is the currently accepted drug for acute SCI. Anti-inflammatory treatments in experimental studies have been shown to be useful on reducing inflammation and preserving spinal tissues for functional improvement. However, the high-dose MP in acute SCI induced a wide range of side effects in clinical trials, which is normally associated with higher incidence of infectious and metabolic complications in comparison to placebo or other treatments. In addition to MP, recent studies have investigated that
Erythropoietin (EPO) can reduce the catastrophe of acute SCI and improve healing process after trauma.
In the present study, we address time series gene expression alteration of spinal cord injury with or without MP or EPO treatment using large oligonucleotide microarray datasets. We illustrated the candidate biological pathways to explain the molecular interactions after treatments such as transcriptional regulation,
protein-protein interactions, and phosphorylation. Finally, our analysis revealed significant molecular events of MP/EPO effects and provided potential
pharmacological therapeutics that directed to rescue damaged tissue and regeneration in the injured spinal cord.
