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目 錄
第一章 前言... 1
第二章 文獻探討... 4
第三章 材料與方法 一、實驗材料與儀器... 12
二、實驗方法... 16
第四章 結果... 35
第五章 討論... 72
第六章 結論... 77
參考文獻... 79
英文摘要... 90
謝詞... 92
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圖目錄
圖 4.1 Effect of various concentrations of gypenosides on AFF
production by human cervix cancer cells (Ca Ski)… … … . 37 圖 4.2 Effect of various concentrations of gypenosides on AAF
production by human cervix cancer cells (HeLa)… … … ... 37 圖 4.3 Effects of incubation timeon AAF production by human cervix
cancer cells (Ca Ski)… … … ... 38 圖 4.4 Effects of incubation timeon AAF production by human cervix
cancer cells (HeLa)… … … . 39 圖 4.5 a-f Effects of various concentrations of gypenosides on the
HPLC profile of N-acetylation of AF by human cervix cancer cells
(Ca Ski)… … … ... 40 圖 4.6 a-f Effects of various concentrations of gypenosides on the
HPLC profile of N-acetylation of AF by human cervix cancer cells
(HeLa)… … … . 42 圖 4.7 Effects of gypenosides on DNA-AF adducts in human cervix
cancer cells (Ca Ski)… … … ... 44 圖 4.8 Effects of gypenosides on DNA-AF adducts in human cervix
cancer cells (HeLa)… … … . 45 圖 4.9 a-b Effects of gypenosides on the expression of NAT mRNA in
human cervix cancer cells (Ca Ski)… … … ....… … 46 圖 4.10 a-b Effects of gypenosides on the expression of NAT mRNA in
human cervix cancer cells... 47 圖 4.11 Down-regulation of NAT gene in human cervix cancer cells
treated with 350 µg/ml gypenosides, assayed by cDNA microarray.. 48 圖 4.12.Amount of acetylation of 2-AF by N.gonorrhoreae NAT with
or without specific concentrations of gypenosides were calculated... 52 圖 4.13 Effects of various concentrations of gypenosides on AAF
production by blood cytosol of SD rats in the presence of 30 mM
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AF after incubation for 18 hours... 53 圖 4.14 Effects of gypenosides on the production of AAF by rat
leukocytes in various concentrations of AF... 55 圖 4.15 a-f DNA-adducts levels in leukocytes, liver, lung, bladder,
colon and uterus of SD rats with gastric intubation of AF and
co-treated with or without gypenosides after 12 and 24 hours... 57 圖 4.16 Mean plasma AF concentration versus time profile in
gypenosides pretreated and control animals ... 59 圖 4.17 Mean plasma AAF concentration versus time profile in
gypenosides pretreated and control animals ... 59 圖 4.18 Distribution of total AF metabolites... 71 圖 4.19 Distribution of total AAF... 71
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表目錄
表 4.1 Effects of gypenosides on human cervix tumor cells’ cytosols
(Ca Ski) NAT activity... 35
表 4.2 Kinetic data for acetylation of AF in human cervix cancer cells’ cytosol (Ca Ski)... 36
表 4.3 Effects of gypenosides on the growth of N. gonorrhoreae... 49
表 4.4 Effects of gypenosides on N. gonorrhoreae NAT activity in vitro... 50
表 4.5 Kinetic data for acetylator of 2-AF in N. gonorrhoreae... 51
表 4.6 Effects of gypenosides on N-acetyltransferase activity in vitro... 54
表 4.7 Michaelis-Menten parameters for NAT activity in various organs from control and Gypenosides treated rats... 56
表 4.8 Distribution of AF metabolites in bladder from SD rats... 61
表 4.9 Distribution of AF metabolites in blood from SD rats... 62
表 4.10 Distribution of AF metabolites in colon from SD rats... 63
表 4.11 Distribution of AF metabolites in kidney from SD rats... 64
表 4.12 Distribution of AF metabolites in liver from SD rats... 65
表 4.13 Distribution of AF metabolites in stomach from SD rats... 66
表 4.14 Distribution of AF metabolites in uterus from SD rats... 67
表 4.15 Distribution of AF metabolites in urine from SD rats... 68
表 4.16 Distribution of AF metabolites in stool from SD rats... 69
表 4.17 Distribution of total AF metabolites... 70 .
