Author(s): Huang, KS (Huang, Kun Shan); Lu, MJ (Lu, Min-Jin); Chen, YS (Chen, Yueh- Sheng); Tsai, FJ (Tsai, Fuu-Jen); Kuo, WW (Kuo, Wei-Wen); Cheng, YC (Cheng, Yi-Chang);
Lin, CC (Lin, Chien-Chung); Tsai, CH (Tsai, Chang-Hai); Huang, CY (Huang, Chih-Yang); Lin, JG (Lin, Jaung-Geng); Ou, HC (Ou, Hsiu-Chung)
Title: Proliferative Effects of Chishao on Schwann Cells are FGF-uPA, and ERK- and JNK- Dependent
Source: AMERICAN JOURNAL OF CHINESE MEDICINE, 37 (6): 1191-1202 2009 Language: English
Document Type: Article
Author Keywords: Chishao; Proliferative Effect; Schwann Cells; Nerve Regeneration KeyWords Plus: FIBROBLAST-GROWTH-FACTOR; PERIPHERAL-NERVE
REGENERATION; CARDIOMYOBLAST APOPTOSIS; IMMUNOLOGICAL ACTIVITIES;
ACIDIC POLYSACCHARIDE; PEONIA-LACTIFLORA; NEURONS; SYSTEM; CANCER;
ROLES
Abstract: This study evaluated the proliferative effects of chishao on RSC96, Schwann cells.
A dose-dependent proliferative effect of chishao was obtained by methylthiazol
tetrazolium( MTT), proliferating cell nuclear antigen (PCNA) Western blotting, and wound healing assays in Schwann cells administered with chishao (0-500 mg/ml), except at 500 mg/ml concentration. The chishao-treated cells also showed a dose-dependent activated fibroblast growth factor-2 (FGF-2) signaling with increased urokinase plasminogen activator (uPA) and decreased plasminogen activator inhibitor-1 (PAI-1), enhanced proliferative proteins, extracellular signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK)- signaling. Using mitogen-actvated protein kinase (MAPK)-signaling chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of chishao on RSC cells were identified to be ERK- and JNK- signaling dependent. Based on the results, applying
appropriate doses of chishao to Schwann cells would be a potential approach for enhancing neuron regeneration.
Addresses: [Ou, Hsiu-Chung] China Med Univ, Dept Phys Therapy, Taichung 404, Taiwan;
[Ou, Hsiu-Chung] China Med Univ, Grad Inst Rehabil Sci, Taichung 404, Taiwan; [Huang, Kun Shan; Chen, Yueh-Sheng; Tsai, Fuu-Jen; Huang, Chih-Yang; Lin, Jaung-Geng] China Med Univ, Grad Inst Chinese Med Sci, Taichung 404, Taiwan; [Tsai, Fuu-Jen] China Med Univ, Dept Pediat Med Res & Med Genet, Taichung 404, Taiwan; [Kuo, Wei-Wen] China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan; [Huang, Chih-Yang] China Med Univ, Grad Inst Basic Med Sci, Taichung 404, Taiwan; [Huang, Kun Shan] Hung Kuang Univ, Dept Nursing, Taichung, Taiwan; [Lu, Min-Jin] Chung Shan Med Univ, Dept Internal Med, Taichung, Taiwan; [Lu, Min-Jin] Chung Shan Med Univ, Dept Microbiol & Immunol, Taichung, Taiwan;
[Cheng, Yi-Chang] Taichung Vet Gen Hosp, Emergency Dept, Taichung, Taiwan; [Lin, Chien-
Chung] Armed Forces Gen Hosp, Dept Orthopaed, Taichung, Taiwan; [Tsai, Chang-Hai] Asia Univ, Dept Healthcare Adm, Taichung 413, Taiwan; [Huang, Chih-Yang] Asia Univ, Dept Hlth
& Nutr Biotechnol, Taichung 413, Taiwan
Reprint Address: Ou, HC, China Med Univ, Dept Phys Therapy, 91 Hsueh Shih Rd, Taichung 404, Taiwan.
E-mail Address: [email protected] Funding Acknowledgement:
Funding Agency Grant Number
China Medical University CMU95-058 CMU95-060
This work was supported by a grant from China Medical University (CMU95-058& CMU95- 060). The authors have declared no conflict of interest statement.
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Publisher: WORLD SCIENTIFIC PUBL CO PTE LTD
Publisher Address: 5 TOH TUCK LINK, SINGAPORE 596224, SINGAPORE ISSN: 0192-415X
DOI: 10.1142/S0192415X09007594
29-char Source Abbrev.: AMER J CHIN MED ISO Source Abbrev.: Am. J. Chin. Med.
Source Item Page Count: 12
Subject Category: Integrative & Complementary Medicine; Medicine, General & Internal ISI Document Delivery No.: 523SA
