BIODEGRADATION OF GASOLINE OXYGENATE METHYL TERT-BUTYL ETHER BY PURE AND MIXED CULTURES
林振寰、張耀南,林啟文
E-mail: 9015661@mail.dyu.edu.tw
ABSTRACT
TO INVESTIGATE THE BIODEGRADABILITY OF METHYL TERTIARY BUTYL ETHER, THE RESEARCH FOCUSED ON THE ACCLIMATION AND ENRICHMENT FOR MTBE UNDER CONTROLLED LABORATORY CONDITIONS FROM A MTBE MANUFACTURING PLANT BIOTREATER. ADDITIONALLY, THE NUTRITIONAL AND ENVIRONMENTAL REQUIREMENTS FOR THE MTBE BIODEGRADATION WERE EXAMINED BY A PURE CULTURE OF ENV425 (ATCC 55798) AND A MIXED CULTURE OF UCD. RESULTS SHOWED THAT THE
MICROBIAL CULTURE (ENV425) WAS ABLE TO DEGRADE MTBE WHEN GROWN IN A MEDIUM CONTAINING BACTO BEEF EXTRACT. HOWEVER, STRAIN ENV425 WAS UNABLE TO DEGRADE MTBE WHEN GROWN IN A MEDIUM CONTAINING D-GLUCOSE AND BACTO PEPTONE EVEN THOUGH THE OPTICAL DENSITY WAS AS HIGH AS 1.1 IN A D-GLUCOSE MEDIUM. RESULTS FROM A BATCH TEST INDICATE THAT UCD CULTURE WAS CAPABLE TO DEGRADE MTBE IN A MINIMAL MEDIUM. HOWEVER, THE MTBE BIODEGRADATION
DECREASED WHEN THE UCD CULTURE WAS GROWN IN A MEDIUM CONTAINING ADDITIONAL NUTRIENTS.
IT WAS ALSO FOUND THAT THE MTBE BIODEGRADATION DECREASED AND THE LAG PHASE WAS
PROLONGED WHEN ADDITIONAL CULTURES WERE PRESENT. RESULTS OF BATCH SUBSTRATE REMOVAL EXPERIMENTS SHOWN THAT MTBE WAS COMPLETELY REMOVED WITHIN 191 HOURS FOR A MICROBIAL CULTURE DERIVED FROM A MTBE MANUFACTURING PLANT BIOTREATER, OVER A SIX-MONTH
ACCLIMATED PERIOD. THE RESULTS FROM ACCLIMATED EXPERIMENTS FOR A LABORATORY-SCALE BIOFILTER REVEALED THAT THE INLET MTBE CONCENTRATIONS AFFECTED THE SUBSTRATE REMOVAL RATES. HOWEVER, THE BIODEGRADATION RATES WERE LOW FOR THE CURRENT BIOFILTER, AND WHICH WAS BELIEVED TO DUE TO THE INSUFFICIENT ACCLIMATION TIME.
Keywords : GASOLINE OXYGENATES, MTBE, BIOFILTER, BIODEGRADATION Table of Contents
第一章 緒論--P1 第二章 文獻回顧--P4 2.1 MTBE之來源--P4 2.2 MTBE之物理化學性質--P6 2.3含氧化合物的作用及主要品 種--P7 2.4 MTBE對人體健康之影響--P8 2.5世界各國對MTBE需求成長情形--P10 2.6 MTBE分解菌之代謝路徑--P12 第三章 MTBE之批次降解試驗--P20 3.1前言--P20 3.2材料與設備--P21 3.2.1試驗材料--P20 3.2.2儀器設備--P21 3.3試驗方法--P22 3.3.1 菌種之來源--P22 3.3.2菌種之培養--P23 3.3.3菌株濃度測試--P23 3.4生物降解試驗--P24 3.4.1 ENV425批次降解試驗--P24 3.4.2 UCD菌種之MTBE降解試驗--P25 3.4.3批次馴化菌種之MTBE降解試驗--P25 3.5氣相層析儀法定量分析--P26 3.5.1標準 曲線--P26 3.5.2 GC-FID分析條件--P26 3.6對照組試驗--P27 3.7氣-液相之平衡--P27 第四章 生物濾床反應系統建造--P33 4.1前 言--P33 4.2生物濾床之設計--P34 4.2.1濾料之選擇--P34 4.2.2影響濾床影響效率之因素--P35 4.3生物濾床反應系統之組裝及規 格--P36 4.3.1濾床相關設備--P37 4.3.2分析儀器--P39 4.4實驗材料及藥品--P40 4.4.1濾料--P40 4.4.2實驗藥品--P40 4.4.3濾料之 篩選--P41 4.4.4微生物之培養--P41 4.4.5濾料調配--P41 4.4.6濾料之性質測定--P42 第五章 結果與討論--P47 5.1 ENV425菌株 降解能力之探討--P47 5.2 UCD混合菌降解MTBE能力之探討--P48 5.3連續馴化培養之菌株降解MTBE之能力測試--P50 5.4 生物濾床之降解測試--P50 5.4.1第一階段生物濾床--P50 5.4.2第二階段生物濾床--P51 第六章 結論與建議--P63 6.1 MTBE之 批次降解試驗--P63 6.2生物濾床之降解力測試--P64
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