Studies on Preparation of Nanocollagen and Properties of Its Products 謝欣蓉、陳明造
E-mail: [email protected]
ABSTRACT
The purpose of this research was to apply the nanotechnology to nanocize collage which was extracted from pig skin, and study its physicochemical properties changes. The collage extracted from the pig skin was dissolved first using enzymes or acid-alkali treatment, which will then be synthesized in a mixture of emulsion and surfactant to reduce a substances particle diameter or possible effect of particle aggregation in the process of forming nano-sized collagen hydrolysates. The collage solution was also sprayed by electrical pressure enhancing conducted sheet vibrating to make micro-nano size drop and take its particle size. The amino acid composition of the collage products was analyzed using automatic amino acid analyzer, the microstructure was measured by scanning electron microscope(SEM) and transmission electron microscope(TEM). And the collage emulsion was prepared with collage hydrolysate added surfactant, and TiO2 and ZnO and its emulsifying capacity and emulsion stability, viscosity, conductivity and anti-UV rays were also determined. The results were as follows: 1. The yield of collagen was higher when pig skin was homogenized two times, the same result was obtained as the extracted collagen was dried by freezing dehydration. 2. The major amino acid-glycine, proline and hydroxyproline of collage were disappeared from the product prepared from the collagen treated with papain and pepsin. And the major amino acids in the collagen hydrolysates were different with different enzymes and hydrolyzing time. 3. It can be obtained the micro-nano sized particles from the extracted collagen was nozzler-sprayed with electric-pressure enhancing conducting sheet vibrating into the hydrophobic receptor. 4. Emulsifying capacity and emulsion stability of collagen emulsion decreased with pH value increased, however, the highest values were reached at pH 3; Viscosity of collagen was decreased as it was hydrolyzed, but it was more stable at pH 7 and 9. Conductivity of the collagen emulsion increased with concentration of the surfactant. 5. No filamentous but rough surface structure was found on the SEM micrograph of nanocollagen powder obtained from the pig skin hydrolyzed by pepsin. It could be found the nano-sized structure in TEM
micrograph. 6. Anti-UV rays absorption effect for the products from papain and pepsin hydrolyzation and addition of nano-TiO2 or ZnO were higher than the native collagen, especially it can be obtained the synergistic effect when nano-TiO2 and ZnO were combined together.
Keywords : 膠原蛋白、奈米膠原蛋白、木瓜酵素、豬皮、胃蛋白?
Table of Contents
封面內頁 簽名頁 授權書... iii 中文摘要... iv 英文摘要... vi 誌 謝... viii 目錄... ix 圖目錄... xii 表目
錄... xiv 1. 前言... 1 2. 文獻回顧... 3 2.1原料
皮... 3 2.1.1 皮之組織學構造... 3 2.1.2 免疫系統... 3 2.2膠原蛋白之簡 介... 5 2.3膠原蛋白之製備... 5 2.3.1 動物性膠原蛋白之萃取與純化... 5 2.4膠原蛋白分子 與纖維結構... 6 2.5膠原蛋白的型式及組成... 8 2.5.1 在化妝品中常使用的方式... 12 2.5.2 膠原 蛋白對人體的功能... 12 2.5.3 膠原蛋白在化妝保養品上的應用... 14 2.6 奈米定義與特性... 14 2.6.1 定義... 14 2.6.2 奈米的單位... 14 2.6.3 奈米材料... 15 2.6.4 奈米材料 特性... 15 2.6.5 奈米材料分類... 15 2.6.6 奈米粒子之製備方法... 16 2.6.7 奈米粒子的 特性... 16 2.6.8 化工上的應用... 16 2.7 奈米鋅、鈦粒子(ZnO、TiO)... 18 2.7.1 奈米粒子 之簡介... 18 2.7.2 隱蔽力... 18 2.7.3 白色顏料... 19 2.7.4 奈米鋅的用
途... 20 2.8 物理的防晒劑... 21 2.9 紫外線... 22 3. 材料與方 法... 23 3.1 實驗材料與方法... 23 3.1.1 實驗材料 ... 23 3.1.2 實驗葯 品... 23 3.1.3 實驗設備... 24 3.2 膠原蛋白製程方法... 25 3.3 一般成分分 析... 27 3.4 胺基酸組成分析... 27 3.5 液滴尺寸大小量測... 28 3.6 電導 度... 28 3.7 乳化力... 28 3.8 乳化安定性... 29 3.9 黏
度... 29 3.10 掃描式電子顯微鏡構造圖之分析... 30 3.11 高解析度穿透式電子顯微鏡觀察... 30 3.12 抗紫外線機能性... 31 4. 結果與討論... 34 4.1 膠原蛋白之產率製程... 34 4.2 胺基酸組成分析... 36 4.3 液滴尺寸大小量測... 39 4.4 電導度... 44 4.5 乳化
力... 47 4.6 乳化安定性... 47 4.7 黏度... 50 4.8 掃描式電子顯微鏡構 造圖... 52 4.9 高解析度穿透式電子顯微鏡觀察... 55 4.10抗紫外線機能性... 60 5. 結
論... 67 參考文獻... 69 REFERENCES
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