國立成功大學「邁向頂尖大學計畫」
延攬優秀人才工作報告表
NCKU’s “Aim for the Top University Project”
Work Report Form for Distinguished Scholars
□續聘continuation of employment
離職resignation100 年 7 月 13 日更新
受聘者姓名
Name of the Employee 楊瑞珠 男 女
Male Female
聘 期 Period of Employment
from 102 年(y) 01 月(m) 01 日(d) to 102 年(y) 03 月(m) 31 日(d) 研究或教學或科技研發與
管理計畫名稱 The project title of research,
teaching, technology development and management
邁向頂尖大學計畫 計畫主持人
（申請單位主管）
Project Investigator (Head of Department/Center)
林以行
補助延聘編號
Grant Number HUA100- 3 - 22 - 197
一、研究、教學、科技研發與管理工作全程經過概述。（由受聘人填寫）
Please summarize the entire research, teaching, or science and technology R&D and management work process (To be completed by the employee)
Chronic hepatitis B virus (HBV) infection can cause hepatocellular carcinoma (HCC). Among the mechanisms of HBV tumorigenesis, the viral oncoprotein HBx has been extensively studied. However, the exact mechanism for the HBx-induced tumorigenesis remains to be established. Recently, the molecular biology of liver regeneration (LR) has been used a potential model to study the mechanism of tumorigenesis. In this study, we therefore investigated the role of LR-related genes in HBx transgenic mice which developed HCC in 100% of transgenic mice at around 16 months. The dynamic expression of LR genes was analyzed at different stages (1M, 3M, 6M, 12M, non-tumor liver, and tumor) by Agilent cDNA array and analyzed by IPA software analyses. The candidate genes were then confirmed by real-time PCR. Consistent with array data, the hepatocyte-derived response genes, NF-B1 (Fig. 1) and c-Myc (Fig. 2), were significantly upregulated at the tumor part in the HBx transgenic livers.
Interestingly, SOCS family, which usually become activated in LR were divided into two opposing extremes in the HBx transgenic livers. SOCS4 and SOCS7 were significantly decreased at the tumor part, while SOCS1 and SOCS2 were increased at the tumor and non-tumor part of HBx transgenic livers, respectively (Fig. 3). Surprisingly, by Western blot assay, the expression of SOCS family was significantly decreased at the tumor part in HBx transgenic livers as compared to non-transgenic livers (Fig. 4). Base on these results, the activation of hepatocyte-derived response genes and the downregulation of SOCS family genes at the advanced stages may define the difference between LR and HBV tumorigenesis of hepatocytes. This dynamic expression profile of genes associated with LR in this model of HBx tumorigenesis should help to clarify the biologic regulation and difference in LR and HBV tumorigenesis.
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Fig. 1 The transcriptional level of NF-B1 was significantly upregulated in the HBx transgenic livers.
Fig. 2 The transcriptional level of c-Myc was significantly upregulated at the age of 1 month and tumor part in the HBx transgenic livers.
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Fig. 3 The expression of SOCS4 and SOCS7 were significantly downregulated at the tumor part, while SOCS1 and SOCS2 were upregulated at the tumor and non-tumor part of HBx transgenic livers, respectively.
Fig. 4 The expression of SOCS family was significantly decreased in HBx transgenic livers
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二、研究或教學或科技研發與管理成效評估（由計畫主持人或單位主管填寫）
Please evaluate the performance of research, teaching or science and technology R&D and management Work: ( To be completed by Project Investigator or Head of Department/Center)
(1)是否達到延攬預期目標？
Has the expected goal of recruitment been achieved?
是，受延攬人之研究有達到延攬之預期目標。
(2)研究或教學或科技研發與管理的方法、專業知識及進度如何？
What are the methods, professional knowledge, and progress of the research, teaching, or R&D and management work?
受延攬人之研究、專業知識及進度皆有達到預期之目標。
(3)受延攬人之研究或教學或科技研發與管理成果對該計畫(或貴單位)助益如何？
How have the research, teaching, or R&D and management results of the employed person given benefit to the project (or your unit)?
受延攬人之研究對該計畫有關肝癌相關之研究極有助益。
(4)受延攬人於補助期間對貴單位或國內相關學術科技領域助益如何？
How has the employed person, during his or her term of employment, benefited your unit or the relevant domestic academic field?
受延攬人之研究於補助期間對本單位或國內相關學術科技領域，尤其是有關肝
癌相關之研究上極有助益。
(5)具體工作績效或研究或教學或科技研發與管理成果：
Please describe the specific work performance, or the results of research, teaching, or R&D and management work:
受延攬人之具體工作績效和研究成果皆符合預期之要求及目標。
(6)是否續聘受聘人? Will you continue hiring the employed person? □續聘Yes □不續聘No
※此報告表篇幅以三～四頁為原則。This report form should be limited to 3-4 pages in principle.
※此表格可上延攬優秀人才成果報告繳交說明網頁下載。
This report form can be downloaded in http://scholar.lib.ncku.edu.tw/explain/
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