The Study of Siah2 in Oral Squamous Cell Carcinoma
Sheng-Nan Kuo, Meng-Wei Liu Abstract
Oral Squamous Cell Carcinomas is the fifth common form of cancer in the world.
The gene SIAH2 is located at 3q25 and the protein translated from SIAH2 involved in ubquitin pathway as one kinds of specificity E3 Ubiquitin ligase. E3 Ubiquitin ligase has much relationship with many cancer researches, and there also has been some studies about anomaly expression of SIAH2 in carcinogenesis. We had detected the whole genome of ten oral patients by aCGH (Array-based Comparative Genomic Hybridization) and found that 80% of oral cancer patients had the situation of SIAH2 copy number amplification. We demonstrate that overexpression of SIAH2 in OSCC cell lines and patients. Furthermore, we investigate the function of SIAH2 by the RNA interference (RNAi) technology in order to evaluate the role of SIAH2 in OSCC. To verify the RNAi-induced expression, we examined SIAH2 mRNA and protein expression by real time RT-PCR, Western blotting and Immunocytochemistry. Our results showed that knockdown of SIAH2 could inhibit cell growth. In the further study, we would like to investigate that genomic aberrations of SIAH2 may contribute to the pathogenesis of OSCC.
