人參及人參皂苷;於純系小鼠之馬兜鈴酸腎炎模型的藥效評估 Effects of ginseng and ginsenosides on aristolochic acid-induced nephropathy in inbred mice
中文摘要
馬兜鈴酸 (aristolochic acid, AA) 在中草藥引起的腎病變中扮演重要的角色。本 研究的目的為藉由投予 AA 引起馬兜鈴酸腎病變 (aristolochic acid nephropathy, AAN)，以評估人參 (ginseng extrat, GE) 及其成份 ginsenoside Rb1、Rd 及 Rg1 對 AAN 的改善效果。
給予純系小鼠 C3H/He (6 week-old male) 3.0 μg/mL AA 當飲用水，連續 56 天，
之後治療組分別經口投予 GE (125、250、500 mg/kg)或純成份 ginsenoside (Rb1、
Rd、Rg1) 5 mg/kg 連續 14 天，對照組給予等量蒸餾水，Normal 組則全程給予蒸 餾水。
藉由測定尿蛋白，尿中 N-acetyl-beta-D-glucosaminidase (NAG) 與血中 blood urea nitrogen (BUN) 及 creatinine，以評估小鼠腎功能；腎組織使用 PAS 染色觀察病 理組織改變，並進行免疫螢光染色 (TGF-β，MMP-9，HGF)，以辨識損傷部位 之特異性抗原。
實驗結果顯示，投予 GE 250 mg/kg，ginsenoside Rb1, Rg1 之治療組的尿蛋白、
NAG、BUN、SCr 及血糖值都有降低；組織學及免疫螢光染色觀察發現腎組織損 傷的情形皆有緩解。根據以上結果發現，人參濃縮劑部分，GE 250 的療效最好，
其次依序為 GE 500 及 GE 125。人參皂苷部分，有效程度依序為 Rg1、Rb1、Rd。
英文摘要
Aristolochic acid (AA) has been demonstrated to play a crucial role in Chinese herbs nephropathy. The purpose of this study was to evaluate the therapeutic effect of ginseng extrat (GE) and its active component, ginsenoside (GS), on AA-induced nephropathy.
AA was dissolved in distilled water (3μg/ml) as drinking water to C3H/He mice (6 week-old male) for 56 days. The treatment groups were administered orally with GE (125, 250, 500 mg/kg) or ginsenosides (Rb1, Rd, Rg1 5 mg/kg) once daily for 14 days.
The control group was administered with distilled water. The normal group was only administered with distilled water throughout the experiment.
Urine protein (UP), urine N-acetyl-beta-D-glucosaminidase (NAG), blood urea nitrogen (BUN) and serum creatinine were determined to evaluate renal function.
Renal tissues were served to histological examination (PAS stain and
immunofluorescence). The antibodies, including TGF-β (transforming growth factor-β), MMP-9 (matrix metalloproteinase-9), HGF (hepatocyte growth factor),
were chosen to recognize the specific antigens in injury sites.
Compared with the control group, urine protein, NAG, BUN, serum creatinine and blood glucose were decreased in the GE 250 mg/kg, ginsenoside Rb1 and Rg1 treatment groups. In histological examination, we observed the alleviation in all treatment groups. According to the study, the effect of GE 250 is superior to the other GE-treated groups and ginsenoside Rg1 has the best effect among all the GS-treated groups.
