Author(s): Chang, WT (Chang, Wen-Teish); Hsieh, CH (Hsieh, Cheng-Hong); Hsieh, HS (Hsieh, Hung-Sheng); Chen, CS (Chen, Chinshuh)
Title: Conversion of crude chitosan to an anti-fungal protease by Bacillus cereus
Source: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 25 (3): 375-382 MAR 2009
Language: English Document Type: Article
Author Keywords: Antifungal; Bacillus cereus; Chitosan; Protease; Pythium ultimum KeyWords Plus: CRAB SHELL POWDER; PSEUDOMONAS-AERUGINOSA K-187;
IMPROVED BIOCONTROL ACTIVITY; TRICHODERMA-HARZIANUM; BIOLOGICAL- CONTROL; CHITINOLYTIC ENZYMES; CARBON SOURCE; PURIFICATION;
FLUORESCENS; SHRIMP
Abstract: Bacillus cereus AU004, isolated from soil samples, secreted a complex of hydrolytic enzymes into the culture broth when it was grown aerobically in a medium containing crude chitosan flakes. The presence of the AU004 culture supernatant substantially influenced the growths of the plant-pathogenic fungi Fusarium oxysporum, F. solani and Pythium ultimum in terms of dry weight. AU004 excreted a protease when cultivated in a medium that contained 4% (w/v) chitosan as the major nutritional source. The protease was purified by sequential chromatography and characterized as a novel extracellularly neutral protease. The protease had an Mr of 28.8 kDa. The optimal pH and temperature for protease activity were 7 and 50A degrees C, respectively. Antifungal activity of the protease was observed using an assay based on the inhibition of spore germination and hyphal extension of the fungal Pythium ultimum. This investigation is the first report of the production of an anti-fungal protease from Bacillus spp.
Addresses: [Chen, Chinshuh] Natl Chung Hsing Univ, Dept Food Sci & Biotechnol, Taichung 40227, Taiwan; [Chang, Wen-Teish; Hsieh, Cheng-Hong; Hsieh, Hung-Sheng] Asia Univ, Dept Hlth & Nutr Biotechnol, Wufeng 500, Taichung County, Taiwan
Reprint Address: Chen, CS, Natl Chung Hsing Univ, Dept Food Sci & Biotechnol, 250 Kuokuang Rd, Taichung 40227, Taiwan.
E-mail Address: chinshuh@dragon.nchu.edu.tw Funding Acknowledgement:
Funding Agency Grant Number
National Science Council, Taiwan NSC95-2221-E-272-002
This work was partially supported by a grant from the National Science Council, Taiwan
(NSC95-2221-E-272-002). Ted Knoy is appreciated for his editorial assistance.
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Publisher: SPRINGER
Publisher Address: 233 SPRING ST, NEW YORK, NY 10013 USA ISSN: 0959-3993
DOI: 10.1007/s11274-008-9901-5
29-char Source Abbrev.: WORLD J MICROBIOL BIOTECHNOL ISO Source Abbrev.: World J. Microbiol. Biotechnol.
Source Item Page Count: 8
Subject Category: Biotechnology & Applied Microbiology ISI Document Delivery No.: 403RN
