一、茶葉粗抽物對腦缺血鼠誘發自發性運動量之影響 如 Fig.4 所示，茶葉粗抽物 100 mg/kg 可增加因腦缺血鼠之自發 性運動量（P＜0.05）。
手術後
Locomotor activity (sec)
0 200 400 600 800
1000 Sham
Ischemia TF-30 mg/kg TF-100 mg/kg TF-300 mg/kg
*
Fig.4 Effects of Theae Folium (TF, 30、100、300 mg/kg) on locomotor activity in ischemic rats.
* P<0.05 compared with ischemia group.
如 Fig.5 所示，茶葉粗抽物 100 mg/kg 可增加因腦缺血鼠之探索 能力（P＜0.05）。
手術後
Entries in Holes (counts)
0 1 2 3 4 5
6 Sham
Ischemia TF-30 mg/kg TF-100 mg/kg TF-300 mg/kg
*
Fig.5 Effects of Theae Folium (TF, 30、100、300 mg/kg) on entries in holes in ischemic rats.
* P<0.05 compared with ischemia group.
二、茶葉粗抽物對腦缺血鼠誘發水迷宮學習記憶障礙之影響 1. 茶葉粗抽物對腦缺血鼠誘發水迷宮空間操作障礙之影響 如 Fig.6 所示，腦缺血四天後，造成大鼠於水迷宮空間性學習操 作之障礙（P＜0.001）。茶葉粗抽物 100、300 mg/kg 可改善因腦缺血 鼠誘發水迷宮學習操作障礙（P＜0.001）。
Fig.6 Effects of Theae Folium (TF, 30、100、300 mg/kg) on total swimming time on the hidden platform of water maze in ischemic rats.
** P<0.01 compared with sham group.
*** P<0.001 compared with sham group.
## P<0.01 compared with ischemia group.
### P<0.001 compared with ischemia group.
2. 茶葉粗抽物對腦缺血鼠誘發水迷宮參考記憶障礙之影響
Fig.7 Effects of Theae Folium (TF, 30、100、300 mg/kg) on total swimming time swum through the target ring of water maze in ischemic rats.
*P<0.05 compared with sham group.
# P<0.05 compared with ischemia group.
3. 茶葉粗抽物對腦缺血鼠誘發水迷宮工作記憶障礙之影響
Fig.8 Effects of Theae Folium (TF, 30、100、300 mg/kg) on total swimming time swum to hidden platform during reacquistion and retrieval of water maze in ischemic rats.
* P<0.05 compared with sham group.
# P<0.05 compared with ischemia group.
4. 茶葉粗抽物對腦缺血鼠在水迷宮非空間性游泳能力之影響 如 Fig.9 所示，腦缺血九天後，可造成大鼠於水迷宮尋找可見逃 逸平台時間增加（P＜0.05）。茶葉粗抽物 30、100、300 mg/kg 可使游 泳時間減少（P＜0.05），但在游泳速度無任何影響。
Swimming speed (cm/sec)
0 TF-100 mg/kg TF-300 mg/kg
***
### ### ###
Fig.9 Effects of Theae Folium (TF, 30、100、300 mg/kg) on total swimming time and speed on the visible platform of water maze in ischemic rats.
*** P<0.001 compared with sham group.
### P<0.001 compared with ischemia group.
三、(-)-Epigallocatechin gallate 對腦缺血鼠誘發自發性運動 量之影響
如 Fig.10 所示，EGCG 10 mg/kg 及正對照藥 PTX 100 mg/kg 對 腦缺血鼠自發性運動量並無影響。
手術後
Locomotor activity (sec)
0 200 400 600 800 1000
Sham Ischemia
EGCG-10 mg/kg PTX-100 mg/kg
Fig.10 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on locomotor activity in ischemic rats.
如 Fig.11 所示，EGCG 10 mg/kg 及正對照藥 PTX 100 mg/kg 對 腦缺血鼠探索能力並無影響。
手術後
Entries in Holes (counts)
0 1 2 3 4 5 6
Sham Ischemia
EGCG-10 mg/kg PTX-100 mg/kg
Fig.11 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on entries in holes in ischemic rats.
四、(-)-Epigallocatechin gallate 對腦缺血鼠誘發水迷宮學習 記憶障礙之影響
1. (-)-Epigallocatechin gallate 對腦缺血鼠誘發水迷宮空間操作障 礙之影響
Fig.12 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on total swimming time on the hidden platform of water maze in ischemic rats.
** P<0.01 compared with sham group.
*** P<0.001 compared with sham group.
## P<0.01 compared with ischemia group.
### P<0.001 compared with ischemia group.
2. (-)-Epigallocatechin gallate 對腦缺血鼠誘發水迷宮參考記憶障 礙之影響
如 Fig.13 所示，腦缺血七天後，造成大鼠於水迷宮參考記憶障礙
（P＜0.05）。EGCG 10 mg/kg 及正對照藥 PTX 100 mg/kg 可改善因腦 缺血鼠誘發水迷宮參考記憶障礙，但統計上並無顯著差異。
Times (sec)
0 3 6 9 12
Sham Ischemia
EGCG-10 mg/kg PTX-100 mg/kg
*
Fig.13 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on total swimming time swum through the target ring of water maze in ischemic rats.
*P<0.05 compared with sham group.
3. (-)-Epigallocatechin gallate 對腦缺血鼠誘發水迷宮工作記憶障 礙之影響
如 Fig.14 所示，腦缺血八天後，造成大鼠於水迷宮工作記憶障礙
（P＜0.05）。EGCG 10 mg/kg 及正對照藥 PTX 100 mg/kg 可改善因腦 缺血鼠誘發水迷宮工作記憶障礙，但統計上並無顯著差異。
Reacquistion Retrieval
Times (sec)
0 30 60 90 120
Sham Ischemia
EGCG-10 mg/kg PTX-100 mg/kg
* *
Fig.14 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on total swimming time swum to hidden platform during reacquistion and retrieval of water maze in ischemic rats.
* P<0.05 compared with sham group.
4. (-)-Epigallocatechin gallate 對腦缺血鼠在水迷宮非空間性游泳 能力之影響
如 Fig.15 所示，腦缺血九天後，可造成大鼠於水迷宮尋找可見逃 逸平台時間增加（P＜0.05）。EGCG 10 mg/kg 及正對照藥 PTX 100 mg/kg 可使游泳時間減少（P＜0.05）。但在游泳速度並無任何影響。
Swimming speed (cm/sec)
0 EGCG-10 mg/kg PTX-100 mg/kg
***
### ###
Fig.15 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on total swimming time and speed on the visible platform of water maze in ischemic rats.
*** P<0.001 compared with sham group.
### P<0.001 compared with ischemia group.
五、(-)-Epigallocatechin gallate 對腦組織抗氧化能力之影響 1. (-)-Epigallocatechin gallate 對腦缺血鼠 Malonyldialdehyde 含
量變化之影響
如 Fig.16 所示，腦缺血可造成大鼠海馬回及紋狀體 MDA 含量 的升高（P＜0.01）。EGCG 10 mg/kg 可降低腦缺血所造成海馬回及紋 狀體 MDA 含量升高之現象（P＜0.05）。但 PTX 100 mg/kg 則無任何 影響。
Cortex Hippocampus Striatum
MDA (nmole TTMP/mg Protein)
0.0
Fig.16 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on malonyldialdehyde level in ischemic rats.
** P<0.01 compared with sham group.
*** P<0.001 compared with sham group.
# P<0.05 compared with ischemia group.
2. (-)-Epigallocatechin gallate 對腦缺血鼠 Superoxide dismutase 活性變化之影響
如 Fig.17 所示，EGCG 10 mg/kg 可降低腦缺血鼠海馬回及紋狀 體 total-SOD 之活性（P＜0.05）。但 PTX 100 mg/kg 則無任何影響。
Cortex Hippocampus Striatum
SOD activity (U/mg Protein)
0.0
Fig.17 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on total-superoxide dismutase activities in ischemic rats.
### P<0.001 compared with ischemia group.
3. (-)-Epigallocatechin gallate 對腦缺血鼠 Glutathione 含量變化 之影響
如 Fig.18 所示，腦缺血可造成大鼠皮質區及海馬回 total-GSH 含 量的減少（P＜0.05）。EGCG 10 mg/kg 可改善腦缺血所造成大鼠海馬 回 total-GSH 含量減少之現象（P＜0.001）及增加紋狀體中 total-GSH 含量（P＜0.001）； 正對照藥 PTX 100 mg/kg 可改善腦缺血造成大鼠 皮質區 total-GSH 含量升高之現象（P＜0.01）。
Cortex Hippocampus Striatum
Glutathione (pmole/mg Protein)
0
Fig.18 Effects of (-)-epigallocatechin gallate (EGCG, 10 mg/kg) and pentoxifylline (PTX, 100 mg/kg) on total glutathione level in ischemic rats.
* P<0.05 compared with sham group.
** P<0.01 compared with sham group.
## P<0.01 compared with ischemia group.
### P<0.001 compared with ischemia group.