第五章 結論與建議
5.2 建議
本研究利用分子生物技術對MBR 進行菌相鑑定與探討，在偵測 上雖可比傳統的微生物培養法來的精確，卻也有其限制，當菌種濃度 過低便無法在後續實驗中將其純化做進一步定序，因此建議後續相關 研究時，可採用多種不同技術同步進行比較與查核。且本研究所使用 之引子對長度約200 bp，在菌種比對上代表性稍嫌不足，建議往後之 相關研究若仍希望藉由 PCR-DGGE 進行菌相鑑定，可設計出長度約 500 bp 的引子對，將會有較高的代表性助於研究。
62
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Phylogenetic relationship
Most closely related sequence GenBank
accession number Bacterial division Identity (%) 10s-A 165 Zoogloea oryzae AB201044 Betaproteobacteria 96 10s-B 167 Aquabacterium sp. AF089857 Betaproteobacteria 94
10s-C Sequencing unsuccessful 10s-D 157 Uncultured Rhodocyclaceae
bacterium AY689091 Betaproteobacteria 85 10s-E 167 Zoogloea oryzae AB201044 Betaproteobacteria 92 10s-F 159 Flexibacteraceae bacterium EU057829 Bacteroidetes 89
10s-G 159 Zoogloea oryzae AB201044 Betaproteobacteria 97 10s-H 161 Candidatus nitrotoga DQ839562 Betaproteobacteria 92 10s-I 161 Uncultured bacterium EF127788 environmental samples 92
10s-J 141 Uncultured bacterium DQ322193 environmental samples 88
10s-K 139 Uncultured bacterium AY754855 environmental samples 91 10s-L 132 Rhodobacter gluconicum DQ363135 Alphaproteobacteria 90 10s-M 154 Uncultured bacterium AY754855 environmental samples 89
10s-N 153 Uncultured bacterium AY754855 environmental samples 90
附錄 A 反應槽前端架設選種槽單元之微生物序列比對列表
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Phylogenetic relationship
Most closely related sequence GenBank
accession number Bacterial division Identity (%) 10.60-A 157 Uncultured bacterium EU239337 environmental samples 100
10.60-B 181 Uncultured bacterium EF061582 environmental samples 96 10.60-C 132 Uncultured bacterium DQ105640 environmental samples 95 10.60-D 169 Uncultured
Chlamydia sp.
DQ903996 Chlamydia 92 10.60-E 158 Uncultured bacterium EF443916 environmental samples 9510.60-F 157 Flavobacterium sp. AM888191 Bacteroidetes 98 10.60-G 174 Uncultured bacterium DQ510105 environmental samples 93
10.60-H 172
Flexibacteraceae bacterium
EU057829 Bacteroidetes 9010.60-I 181 Zoogloea oryzae AB201044 Betaproteobacteria 95 10.60-J 142 Uncultured bacterium EU491294 environmental samples 90 10.60-K 177 Uncultured Bacteroidetes
bacterium DQ365987 Bacteroidetes 95
10.60-L 177 Zoogloea sp. DQ413148 Betaproteobacteria 94 10.60-M 172
Dechloromonas sp.
DQ413167 Betaproteobacteria 96 10.60-N 162 Unidentified bacterium EF605933 environmental samples 89 10.60-O 159Zoogloea sp.
DQ413148 Betaproteobacteria 89 10.60-P Sequencing unsuccessful73
附錄 C 反應槽 SRT 為 30 天之微生物序列比對列表
PCR-DGGE band
Sequence length (bases)
Phylogenetic relationship
Most closely related sequence GenBank
accession number Bacterial division Identity (%) 30-A 157 Zoogloea oryzae AB201044 Betaproteobacteria 95 30-B 167 Aquabacterium sp. AF089857 Betaproteobacteria 93
30-C 141 Bdellovibrio sp. AY294216 Deltaproteobacteria 95 30-D 159 Flexibacteraceae bacterium EU057829 Bacteroidetes 89
30-E 158 Zoogloea oryzae AB201044.1 Betaproteobacteria 91 30-F 159 Zoogloea oryzae AB201044 Betaproteobacteria 96 30-G 161 Candidatus nitrotoga DQ839562 Betaproteobacteria 92 30-H 161 Uncultured bacterium EF127788 environmental samples 92
30-I 161 Uncultured alpha proteobacterium AJ416667 Alphaproteobacteria 88 30-J 141 Uncultured alpha proteobacterium EU272976 Alphaproteobacteria 91
30-K 132 Rhodobacter gluconicum DQ363135 Alphaproteobacteria 90
74
Phylogenetic relationship
Most closely related sequence GenBank
accession number Bacterial division Identity (%) M-A 166 Uncultured Sphingobacteria
bacterium EF438202 Bacteroidetes 93
M-B 166 F.ferrugineum M62798 Bacteroidetes 94
M-C 164 Comamonadaceae bacterium EF103206 Betaproteobacteria 90 M-D 171 Bacteriovorax sp. EF092443 Deltaproteobacteria 82
M-E 161 Flexibacter flexilis AJ871243 Bacteroidetes 82 M-F 164 Prosthecobacter vanneervenii AJ966883 Verrucomicrobia 93 M-G 164 Zoogloea oryzae AB201044 Betaproteobacteria 93 M-H 151 Uncultured bacterium AY250094 environmental samples 89
M-I 161 Uncultured alpha proteobacterium AJ416667 Alphaproteobacteria 88 M-J 180 Uncultured bacterium EU135183 environmental samples 80 M-K 165 Uncultured Flexibacteraceae
bacterium DQ507162 Bacteroidetes 89
M-L Sequencing unsuccessful