5-2-1 建立系統性參數
未來可針對基材建立系統性之參數，研究分析出不同之參數，所 製作出電紡絲之纖維形態之差異，在統整之後，便可以製造出一致性 高、不同直徑大小之奈米電紡絲，會更有利於實驗數據之比較與分析。
5-2-2 收集器之改良
本研究所製作出之奈米電紡絲纖維走向皆為亂數分佈不纖布形 態，這是由於電紡平台之收集器的部分並無進行改裝，所以並無法控 制奈米纖維落在收集器上時之方向性，在過去的一些研究中指出，可 以利用不同的纖維收集方式以控制奈米纖維之方向性。第一種設置是 將靜態的金屬收集器置換成一可轉動之金屬滾筒收集器，由於滾筒轉 動的力量使得纖維得以平行排列(圖 5 - 1a)，以此方式可製做出平行 排列纖維的薄膜(Chew et al., 2005; Matthews et al., 2002)。第二種設置 是將滾筒的長度縮小或是在滾筒上纏繞金屬線(圖 5 - 1bc)，由於電紡 絲收集的範圍變小了，所以可以製作出線狀的電紡絲，但長度只有滾 筒的周徑長(Bhattarai et al., 2005; Xu et al., 2004)。第三種設置方式則 可製作出平行排列之連續電紡絲線，電紡絲先噴在置有金屬收集器的
收集(圖 5 - 2) (Khil et al., 2005; Smit et al., 2005)。
(a) (b) (c)
圖 5 - 1：(a)滾輪式電紡裝置；(b)金屬線圈式電紡裝置；(c)圓盤式電 紡裝置。
圖 5 - 2：平行排列連續電紡絲線之電紡裝置(Smit et al., 2005)。
5-2-3 材料選擇
未來研究可以選擇具有生物可降解性的材料來製作奈米電紡 絲，如：聚乳酸-甘醇酸(PLGA)、左旋型聚乳酸(PLLA)、聚己內酯 (PCL)、膠原蛋白…等。依據不同的需求而使用不同的材料來製作奈
米纖維細胞支架，將可以增加未來組織工程可應用之範圍。
5-2-4 加強基材強度及力學評估
加強基材之強度，可以增加基材之耐用性，將會是可否進行進一 步體內動物實驗之重要因素，而加強強度之方式有許多種，如交聯、
編織等。因此，未來可以研究不同加強基材強度的方式對於強度增加 及細胞反應之影響，以找出最合適之加強基材強度方式。
5-2-5 生物反應器
當基材與細胞相容性之研究達到不錯的效果時，可以著手開發生 物反應器，以模擬出體內之環境，藉由生物反應器之研究，可以了解 在模擬體內環境之後，對於細胞之生長反應、細胞外基質之分泌是否 能達到更好的效果，甚至可以減少人工組織植入體內後，與植體與體 內整合之時間。
5-2-6 幹細胞培養
雖然纖維母細胞擁有不錯的合成膠原蛋白之能力，但其分化能力 是受到限制的，若使用此一種類之細胞，將會縮小組織工程可應用之 領域。因此，使用幹細胞培養將會是不錯的選擇，由於幹細胞有非常 好的分化能力及自我更新的表現。除此之外，幹細胞也有非常穩定的
織培養，應該會有更好的成效。
5-2-7 動物試驗
以目前的科技發展，以人工的方式畢竟還是無法完全模擬出體內 之環境，因此，最後的願景當然還是能夠進行動物試驗，對於臨床之 應用才有足夠之意義。
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