建議

1. 本研究使用能源作物向日葵為實驗植體，若只考量後續帶來之能

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源效益，未來研究應可找尋與向日葵同屬於生質能源之油菜及玉米 等作物。

2. 本研究生長激素添加方式為噴灑至葉片濕潤，從 IAA 組別來看其 生長情形並無有效提升，未來可考量直接配置溶液澆灌至土壤等不 同之添加方式。

3. 本研究添加過氧化鈣於水耕中效益不佳，以包膜方式添加入水體 中之方式會因長期浸泡於水裡造成膜快速溶解，須考量其他添加方 式取代於包膜添加。

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意見回復

口試委員 高志明 老師：

1. 使用之化學藥劑之間會有交互作用，彼此之間之效果也許是抵制 或為增加效益，哪個組合與控制組比較為較佳?

回覆: 生長激素濃度為 10^-8M 時為最有效果之組合。

2. 添加過氧化鈣考慮其效果與經濟考量下，是否有其必要性?

回覆: 過氧化鈣於水耕試驗中效果不佳，但其在土壤中不僅僅扮演著 釋氧劑之角色，其加入還可改變土壤中 pH 值，進一步改善目前現地 中土壤普遍酸化之問題。

3. 植物促生菌如何添加比較有效果?

回覆: 添加植物生長激素為預期增進土壤中促生菌更加活躍以及提 供植體本身植物荷爾蒙使其促進生長，而本研究中添加生長激素最有 效果之濃度為 10^-8M。

4. 添加過氧化鈣是否會有阻塞問題，以及 pH 上升之影響?

回覆: 本研究添加於水耕試驗中產生問題為釋放氧氣及 pH 上升速度 過快，不僅不適合耗時較長之植生復育實驗，且因係水耕試驗，將植 體長時間浸泡於 pH 高之溶液環境中也會造成損害，但若應用於土壤 環境中，可預期改變土壤中酸化之問題。

5. 為何選擇向日葵，而不是玉米或是其他能源作物?

回覆: 因高雄大學本身已有向日葵花田，於實驗作物上之採集以及於 實驗設計上可更接近於實場環境，而未來本實驗室後續研究可於考量 到後續粹油效益上來選用其餘能源作物做為比較，感謝委員寶貴意 見。

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6. 為何選擇噴灑於葉片上，而非直接澆灌?

回覆: 因參考許多文獻皆是以噴灑葉片方式來做為添加，經證明其結 果也是較其餘添加方式有效，而本研究也於實驗初期以培地茅植體做 為實驗比較不同添加部位之效果，也為噴灑葉片之組別較為有效。

7. 非點源噴灑是否有污染上之疑慮?

回覆: 因其濃度(10^-8M)比起其餘化學藥劑濃度來說屬較低濃度，但若 考慮到噴灑上污染之疑慮，未來應於實驗設計時，將植體皆安置於一 溫室中，應可有效減低此一疑慮。

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口試委員 董正釱 老師：

1. 數據總結來說，不同濃度下之最佳濃度為何?

回覆: 生長激素濃度為 10^-8M 時為最有效果之組合。

2. 在哪個金屬下效果更好?

回覆: 本研究只針對重金屬銅來做為整治目標重金屬。

3. 可考慮於複合重金屬環境中，比較其整治多種重金屬之效果。

回覆: 感謝委員寶貴意見，實驗室後續研究中應可考慮添加不同重金 屬來達到一複合重金屬環境比較其整治效果。