（4.193）；其他被活化的基因包括 Homo sapiens U5 snRNP-specific protein, 116KD（U5-116KD）mRNA；Myosin VI 等；其下調（Down）的基 因中，最受抑制的是；Aquaporin 6(4.193），kidney specific；其他包 括 Ribosomal protein S17；ALEX1protein 等。
(2)標準化，標準化組受到活化上調的基因中，表量最大的基因是 ME1；AARS；
GGCX；RPP2；UBA2；CHUK 等。標準化組下調基因中最受抑制的是 NAPK14 等。
6、以作用機轉而言，以未標準化實驗組 對照組（DADS/DMSO）的 Ratio of mean 數值來分析基因受到 DADS 活化（up）或抑制（down）：以 Apoptosis 而言，
上調基因（＞2.0）有 DAP-1（2.25），TRIP9（2.07）。下調基因有 Apaf-1(0.48)、
Bf1-1(0.42)、BCL2-related(0.39)、Daxx(0.38)、DENN(0.32)。DAP-1(2.25)
是 Apoptosis 基因，BCL2-related(0.39)及 DENN(0.32)是 Anti-Apoptosis，
即大蒜素 DADS 對於大腸癌細胞株 Colo 205 的細胞分裂調控，有一部份是透 過 Apoptosis 的作用，而且是經由促進凋亡基因(DAP-1)的活化及抑制抗凋 亡基因(BCL2 及 DENN)的共同參與作用來完成達到殺死 Colo 205 癌細胞，如 圖 25-2730,47，即大蒜素 DADS 會經由活化 Apoptosis 的基因（DAP-1）及抑制 抗 Apoptosis 的基因（BCL2 及 DENN）共同作用，而造成大腸癌細胞株的死 亡。
7、經由 cDNA microarray 晶片數值，DADS 可能通過調控癌相關基因表達以及通 過阻抑癌細胞周期進程程等途徑來抑制大腸細胞株 Colo 205 癌細胞增殖，
誘導抑制癌細胞分化及凋亡。有多個基因參與了大蒜素 DADS 毒殺 Colo 205 的作用，這些基因如同雨後春筍，長短不一，其公用有向上增生及向下抑制 調控功能的不同作用，除了 Apoptosis 的基因外，其他如 Oncogene 抑癌基 因（rho G 0.24）；V-ral simian leukemia viral oncogene homolog B （ras related；GTP binding）（0.2），表現多受到抑制，相對的抑制大腸細胞株 Colo 205 癌細胞增殖。
8、未標準化及標準化（normalization）的基因表現個體及表現量及 ratio of
（DADS/DMSO）的數值，是會因標準化及為標準化此二種不同的統計方法，
而呈現不同的結果，因此，以後如何二者取其一，是否全部標準化，以求比 較客觀方法，仍是以後必須繼續探討的問題。
9、Cy5（紅光）635nm 和 Cy3（綠光）532nm 實驗會因結合標識的螢光染劑不同，
而有不同結果，實際必須互相交換做兩次，才不會有因結合不同染劑而造成 誤差，但因目前 Microarray 晶片仍偏貴，且因限於經費，因此未能多做幾 次，以後如有更多的時間及經費，將繼續從事此方面的研究。
10、凋亡基因(Apoptosis)及抗凋亡基因(Anti-Apoptosi)共同參與 DADS 調控 Colo 205 細胞株的作用，中間還包括 p5326,29,61。凋亡基因及抗凋亡基因的共 同拮抗作用，就如同日月對於萬物生長的調控，亦猶如中醫的陰陽對於人的 體質生長之調控。二者相互消長，促使萬物生長平衡，永保健康，也就是內 經所謂【陰平陽秘，有為乃治】，陰陽調和，恰猶如凋亡基因及抗凋亡基因 互相拮抗，以維持細胞的穩定，不致癌化。其他有關實際臨床上 DADS 對人 體癌症治療的效果及機轉，仍有待日後進一步探討。
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