一、PART I 貓：組織免疫學染色篇
麻醉的貓右側肝、膽經之期門、日月穴經電針 20 分鐘後，經組織免 疫學染色後發現膽囊、奧迪氏括約肌、十二指腸CCK 分泌細胞及 VIP 神 經表現增強。
二、PART II 兔子：濃縮中藥木香檳榔丸篇
麻醉的兔子經鼻胃管灌食不同劑量的木香檳榔丸（0.25 gm；0.5 gm；1.0 gm；2.0 gm）60 分鐘內對兔子的奧迪氏括約肌有不同程度之調 控，且有藥物劑量之依存性；其作用機轉經由興奮副交感神經muscarinic M1 receptors。
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Effect of Electroacupuncture and Muh-Shiang Bin-Lang-Wan on Sphincter of Oddi in Anesthetized Animal：
Immuohistochemistry and Mechamism Studies
Kuo Yung-Ling Advisor: Lin Jaung-Geng
Institute of Chinese Medical Science of China Medical University
The sphincter of Oddi (SO) plays an important role in regulating the bile flow into the duodenum. According to Chinese literatures, many chest and abdomen acupoints and Chinese medicine drugs could treat acute abdomen diseases。Our previous studies demonstrated that electroacupunture (EA) modulated the SO motility in rabbits and cats through activation of non-adrenergic non-cholinergic (NANC) pathway。Now； We had designed two studies to investigate the changes of SO
Part I：This study designed to investigate the changes of neurotransmitters such as CCK and VIP in lower biliary tract in cats receiving EA stimulation.
After cats were anesthetized with intramuscular injection of ketamine hydrochloride, they were prepared to conduct EA stimulation on right Qimen (LR14) and Riyue (GB24). The parameters of EA were 6 pulses/ 3 sec and 45 pulses/ 3sec alternatively in frequency, 1-2 mA in intensity and 20 min in stimulation duration. After the comptleteness of EA stimulation, visceral organs such as gallbladder, duodenum and the sphincter of Oddi were removed and frozen for immunohistochemistry localization of CCK and VIP.
The results showed that the distribution of CCK-labelled cells in duodenum, gallbladder and SO were more and distinct after EA than before EA stimulation. Whereas, the VIP-labelled cells were significantly more and distinct in duodenum and SO, but not in gall bladder. We conclude that EA regulates the billiary motility though increasing the distribution of CCK- and VIP- containing cells in duodenum and the sphincter of Oddi.
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Part II：This study was designed to investigate the effect of Chinese Medicinal Herbs MUH-SHIANG-BIN-LANG-WAN (MSBLW) and their mechanism of action on regulating the motility of SO in rabbits. The activity of SO in anesthetized rabbits was measured by using a continuously perfused open-tip manometric method. The rabbits were crammed with different doses of MSBLW through naso-gastric tubes. The SO motility before and after the administration of MSBLW were recorded, and analyzed with a computer equipped with an off line analysis software. The results showed that the SO activity, in terms of tonic pressure and phasic contraction pressure, were significantly changed with a dose-dependent manner. A significant lower tonic pressure and a higher phasic contraction pressure were noticed 40-60 min after administration of MSBLW with the peak response at 0.5-1.0 gm range. The responses were blocked by pretreatment of muscarinic receptors (M1) antagonist, pirenzepine (10mg/kg). We conclude that MSBLW is effective in increasing the SO motility through activation of M1 muscarinic receptors, which provides an evidence-based application of MSBLW in the treatment of biliary disorders.
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謝 辭
中醫所博士班 5 年半來的求學過程裏，歷經了匆忙奔波勞累但又 快樂的研究實驗過程，但因堅持投稿的品質也歷經近2 年期待的煎熬，
當下在寫謝辭中回憶起往事百感交集。
感謝，指導老師林昭庚教授多年來的指導與贈送中西醫病名對照大辭典 一書中”盼為中醫立言”之勉勵；謝慶良教授研究治學嚴謹的態度令我受 益匪淺；邱仁輝教授在陽明大學傳醫所針灸實驗室，提供最大的支持，
使我在研究實驗過程中每周在台北3 天的時間能密集做實驗，順利完成 學業。
感謝，博士口試中，張永賢副校長、陳光偉所長、李德茂主任及成功大 學鄭瑞棠教授細心的指導與建言。
感謝，論文修改中，陳悅生副教授及周美惠助教細心逐字的校正使得以 完美呈現。
感謝，求學行醫生涯中，一直諄諄教導提攜的鍾永祥老師，張步桃老師 及陳榮洲教授。
最後感謝，家人多年來的支持與諒解，謹以此冊論文之成果獻上。
現今，博士學業已告一段落，但我仍不斷的在享受 5 年半來點點滴滴的 求學過程，不管其酸甜苦辣，因為我為夢想付諸於行動。
勇麟 感謝您們
2003/12/25