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Effect of silicone rubber chamber filled with Bletilla
striata on regenerating rat sciatic nerve
Li-Ju Lin
Major professor：Yueh-Sheng Chen
Institute of Chinese Medical Science, China Medical University
Application of combining herbal medicine and biomedical material science to nerve regeneration is a new approach. In this report, we
describe a novel use of extraction of hyacinth bletilla, as an extracellular matrix for peripheral nerve regeneration. In the in-vitro test, PC 12 cells were cultured to test the extraction’s growth-promoting capability. In the in-vivo test, a 15-mm gap of rat sciatic nerve was created between the proximal and distal nerve stumps, which were sutured into silicone rubber tubes filled with the hyacinth bletilla gel. As a result, we found that the extraction of hyacinth bletilla could promote the growth and extension of neurites of the PC 12 cells. However, this growth-promoting effect was not seen in the in-vivo test. In addition, we found the recovery of the motor function was slower than that of the sensory function in the regenerated nerve fibers.
Key word: , nerve regeneration, silicon rubber tube, matrix
致謝
本論文由衷地感謝陳悅生老師的細心耐心指導與指正。感謝徐 媛曼老師提供寶貴意見，李采娟老師於統計學上指導。另外，謝謝彰 化基督教醫院神經外科鄭均洹醫師於神經外科技術上協助、台灣養猪 科學研究所游碧蓮小姐於神經切片上的協助、實驗室杜寧漪小姐於細 胞實驗上指正及協助、向士偉醫師於動物實驗上協助。
最後，僅以此論文獻給一直在精神上支持我的家人。