本篇研究目的就是要探討苯甲基異硫氰酸酯和苯乙基異硫氰酸酯對 於人類前列腺癌細胞株DU-145 誘導細胞凋亡的機轉。由實驗的結果 可得知，苯甲基異硫氰酸酯誘導人類前列腺癌細胞株 DU-145 產生凋 亡的機轉，主要是使細胞週期停滯在G2/M phase，並且使細胞 DNA 損傷而無法修復，細胞大量釋放ROS 和鈣離子，造成細胞的粒線體 膜電位下降，使粒線體釋放出凋亡因子，以及造成內質網的氧化壓 力，以及 caspase-3 和 caspase-9 的活性上升，最後誘導細胞走向凋亡。
而苯乙基異硫氰酸酯誘導人類前列腺癌細胞株DU-145 產生凋亡的機 轉，則有些微不同，主要也是使細胞週期停滯在G2/M phase，並且 使細胞DNA 損傷而無法修復，使細胞大量釋放 ROS 和鈣離子，會造 成細胞的粒線體膜電位下降，使粒線體釋放出凋亡因子，以及造成內 質網的氧化壓力，以及活化Fas 和 Fas ligand，造成 caspase-3、
caspase-8 和 caspase-9 的活性上升，最後誘導細胞產生凋亡。
苯甲基異硫氰酸酯誘導人類前列腺癌細胞株DU-145 產生凋亡的機 轉，主要是透過ROS 的大量產生，造成內質網壓力，以及粒線體膜 電位的下降，使細胞走向粒線體凋亡路徑。而苯乙基異硫氰酸酯誘導 人類前列腺癌細胞株DU-145 產生凋亡的機轉，則是透過 ROS 的大 量產生，造成內質網壓力，活化死亡接受器Fas 和 Fas ligand，使細
胞走向死亡接受器凋亡路徑，以及粒線體膜電位的下降，使細胞走向 粒線體凋亡路徑。
總結以上的結論，苯甲基異硫氰酸酯和苯乙基異硫氰酸酯會造成人類 前列腺癌細胞株DU-145 細胞週期停滯，並且走向凋亡，證實苯甲基 異硫氰酸酯和苯乙基異硫氰酸酯對於人類前列腺癌細胞株DU-145 有 抗癌的效果，因此苯甲基異硫氰酸酯和苯乙基異硫氰酸酯在未來應用 在癌症患者的治療上具有無限的潛力。
BITC
G2/M phase arrest G2/M phase arrest
Cyclin B
GRP78 MMP↓MMP↓
Ca2+
G2/M phase arrest G2/M phase arrest
Nuclear
Bid tBidtBid
圖5-2 苯乙基異硫氰酸酯誘導人類前列腺癌細胞株 DU-145 走向細胞 凋亡的機轉
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