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第六章 圖表（Figure）
Figure 1. Curcumin inhibits the enzymatic activity of Src and FAK.
IV-5 cells were treated without or with different concentrations of curcumin (20, 50 and 100 μM) for different periods of time (24 hrs or 48 hrs ). Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotting, using the antibodies as indicated.
Figure 2. Two Chinese herbs inhibit the enzymatic activity of Src and
FAK in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.21-32) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.29 and 30 exhibited anti-Src and –FAK activity.
Figure 3. One Chinese herb can significantly reduce the level of Src
Pi-Y416 and FAK Pi-Y861 in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.62-78) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.78 exhibited anti-Src and –FAK activity.
Figure 4. One Chinese herb can supress the Src and FAK kinase
activity in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.79-90) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.86 exhibited anti-Src and –FAK activity.
Figure 5. One Chinese herb had inhibitory effect on activition of Src
and FAK in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.192-218) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.195 exhibited anti-Src and –FAK activity.
Figure 6. One Chinese herb can significantly reduce the level of Src
Pi-Y416 and FAK Pi-Y861 in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.139、201-214) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.139 exhibited anti-Src and –FAK activity.
Figure 7. One Chinese herb can supress the Src and FAK kinase
activity in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.228-240) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.232 exhibited anti-Src and –FAK activity.
Figure 8. Two Chinese herbs inhibit the enzymatic activity of Src and
FAK in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.241-254) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.249 and 250 exhibited anti-Src and –FAK activity.
Figure 9. One Chinese herb can supress the Src and FAK kinase
activity in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.268-279) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.273 exhibited anti-Src and –FAK activity.
Figure 10. One Chinese herb can supress the Src and FAK kinase
activity in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.96、319-333) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.96 exhibited anti-Src and –FAK activity.
Figure 11. One Chinese herb can supress the Src and FAK kinase
activity in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.49-60) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Sample No.51 exhibited anti-Src and –FAK activity.
Figure 12. Two Chinese herbs can supress the proliferation of IV5
cells in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest for 48 hrs. Reduced cell number and altered cell morphology were detected in IV5 cells treated with interested herbs.
Figure 13. Three Chinese herbs can supress the survival of IV5 cells
in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest for 48 hrs. Reduced cell number and altered cell morphology were detected in IV5 cells treated with interested herbs.
Figure 14. Three Chinese herbs can supress the survival of IV5 cells
in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest for 48 hrs. Reduced cell number and altered cell morphology were detected in IV5 cells treated with interested herbs.
Figure 15. Three Chinese herbs can supress the proliferation of IV5
cells in this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest for 48 hrs. Reduced cell number and altered cell morphology were detected in IV5 cells treated with interested herbs.
Figure 16. Two Chinese herbs can supress the survival of IV5 cells in
this assay.
IV-5 cells were treated with or without 1% Chinese herbs of interest for 48 hrs. Reduced cell number and altered cell morphology were detected in IV5 cells treated with interested herbs.
Figure 17. Comparison of cell proliferation among the cell treated
with different extracts.
IV-5 cells were treated with or without 1% Chinese herbs of interest for 48 hrs. Reduced cell number and altered cell morphology were detected in IV5 cells treated with interested herbs.
Figure 18. Screen for the extracts that can supress the amount or
enzymatic activity of Src and FAK.
IV-5 cells were treated with or without 1% Chinese herbs of interest (sample No.1-333) for 48 hrs. Lysates (50 μg) from each group were analyzed by SDS-PAGE and western immunoblotted with antibodies as indicated. Lysates from IV5 cells that incubated with curcumin (100 μM) for 24 hrs were utilized as a positive control. Candidate extracts exhibited anti-Src and –FAK activity and suppressed the expression level.
Figure 19. Twelve Chinese herbs that retain anti-Src and –FAK
activity can effectively reduce the cell proliferation in IV5 cells.
附圖一： The involvement of Src in a variety of cell physiology.
Src activation by several pathways can lead to diverse effects on a tumor’s clinical behavior. Interruption of Src signaling can potentially decrease a tumor’s size and growth rate, its ability to metastasize, and its ability to induce bone pain and fractures (Finn, 2008).
附圖二：
Src and focal adhesion kinase are two therapeutic targets in cancer treatment.
Src and FAK integrate signals from both integrins and growth factor receptors on the cell membrane. Src is activated by autophosphorylation on tyrosine 416 while FAK is autophosphorylated on tyrosine 397. This acts as a high affinity binding site for the SH2 domain of Src that in turn phosphorylates FAK on a number of additional tyrosine residues. These then act as docking sites for a number of signalling intermediates. FAK also contains a N-terminal FERM domain and proline rich regions while Src has an SH2 and SH3 domain all of which facilitate protein–protein interactions and activation of a diverse range of signalling pathways
(Brunton and Frame, 2008).