圖 4-2 ApoE 剔除鼠餵食實驗飼料第 0 至 12 週三酸甘油酯濃度
fig.4-2 Serum level of triglyceride in ApoE knockout mice fed the experimental diets for 12 weeks.
1.Values are means ± S.E.M. mg/dl.
2.Data were analyzed by Student’s t-test.
圖 4-3 ApoE 剔除鼠餵食實驗飼料第 0 至 12 週血液膽固醇濃度
fig.4-3 Serum level of total cholesterol in ApoE knockout mice fed the experimental diets for 12 weeks.
1.Values are means ± S.E.M. mg/dl.
2.Data were analyzed by Student’s t-test.
圖 4-4 ApoE 剔除鼠餵食實驗飼料後血液 NEFA 濃度
fig.4-4 Serum level of NEFA in ApoE knockout mice fed the experimental diets for 15 weeks.
1.Values are means ± S.E.M. mg/dl.
2.Data were analyzed by Student’s t-test.
圖 4-5 ApoE 剔除鼠餵食實驗飼料 15 週後腎臟中總膽固醇及三酸甘油酯濃度 Table 4-5 Renal total cholesterol(TC) and triacylglycerol(TG) concentrations in ApoE knockout mice fed the experimental diets for 15 weeks 1.Values are means ± S.E.M. mg/dl.
2.Data were analyzed by Student’s t-test.
A
B
圖 4-6 ApoE 剔除鼠餵食實驗飼料 12 週後頭臂動脈 Intima / Media area ratio
Fig.4-6 intima/media ratio of brachiocephalic artery in ApoE knockout mice fed the experimental diets for 15 weeks.
1.Values are Mean±SEM
2. Data were analyzed by Student’s t-test.
圖 4-7 ApoE 剔除鼠餵食實驗飼料 12 週後主動脈損傷情形
Fig.4-7 Atherosclerotic lesion formation in ApoE knockout mice fed the experimental diets for 15 weeks.
The aortic arch were dissected out and examined for atherosclerotic lesion formation using the en face technique. A, two representative aortae from the HF+BEA and HF group. B, the extent of atherosclerotic lesion formation in each mouse, expressed as percentage of aortic surface area covered by atherosclerotic lesions. The mean atherosclerotic lesion area of each group of mice is indicated by a horizontal bar with mean value shown next to it.
A
B
圖 4-8 ApoE 剔除鼠餵食實驗飼料 12 週後肝臟切片(蘇木精-伊紅染色)
Fig 4-8 The lipid accumulation in the liver sections stained by hematoxylin-eosin of ApoE knockout mice fed the experimental diets for 15 weeks.(scale bar=100μm)
圖 4-9 ApoE 剔除鼠餵食實驗飼料 12 週後頭臂動脈血管切片(蘇木精-伊紅染色) (scale bar=100μm)
Fig 4-9 The brachiocephalic artery stained by hematoxylin-eosin of ApoE
knockout mice fed the experimental diets for 15 weeks.(scale bar=100μm)
圖 4-10 ApoE 剔除鼠餵食實驗飼料 12 週後 periodic acid-Shiff (PAS)染色染色之腎 臟切片
Fig 4-10 The kidney sections stained by periodic acid-Shiff (PAS) of ApoE knockout mice fed the experimental diets for 15 weeks.
圖 4-11 ApoE 剔除鼠餵食實驗飼料 12 週後對於頭臂動脈動脈表現 MMP-9 之影 響
Fig. 4-11 Immunohistochemical analysis of MMP-9 in brachiocephalic artery in ApoE knockout mice fed the experimental diets for 15 weeks.(scale bar=100μm)
圖 4-12 ApoE 剔除鼠餵食實驗飼料 12 週後對於頭臂動脈動脈表現 IL-1β之影響 Fig. 4-12 Immunohistochemical analysis of of IL-1β in brachiocephalic artery in
ApoE knockout mice fed the experimental diets for 15 weeks.(scale bar=100μm)
圖 4-13 ApoE 剔除鼠餵食實驗飼料 12 週後對於頭臂動脈動脈表現 HO-1 之影響 Fig. 4-13 Immunohistochemical analysis of of HO-1 in brachiocephalic artery in
ApoE knockout mice fed the experimental diets for 15 weeks.(scale bar=100μm)
圖 4-14 ApoE 剔除鼠餵食實驗飼料 12 週後對於頭臂動脈動脈表現 TM 之影響 Fig. 4-14 Immunohistochemical analysis of of TM in brachiocephalic artery in
ApoE knockout mice fed the experimental diets for 15 weeks.(scale bar=100μm)
圖 4-15 苦瓜乙酸乙酯萃物對 ApoE 剔除鼠胸主動脈中 MMP-9 蛋白質表現量的影 響
Fig. 4-15 Effects of ethyl acetate extracts of bitter gourd on the protein expression of MMP-9 in thoracic aorta.
Tissue protein was collected for MMP-9 analysis using western blots. Amounts of IL-1β were quantified using β-actin as internal control.Values are means ± S.D, HF+BEA group n=5,. HF group n=6 .Data were analyzed by Student’s t-test.
圖 4-16 苦瓜乙酸乙酯萃物對 ApoE 剔除鼠胸主動脈中 IL-1β蛋白質表現量的影響 Fig. 4-16 Effects of ethyl acetate extracts of bitter gourd on the protein expression of IL-1β in thoracic aorta.
Tissue protein was collected for IL-1β analysis using western blots. Amounts of IL-1β were quantified using β-actin as internal control. Values are means ± S.D, HF+BEA group n=5,. HF group n=6 .Data were analyzed by Student’s t-test. *Significantly different from the other group. (p < .05)
第四節 討論與結論
0.00123％Thiazolidinedione (n=5)，餵食 18 週，結果發現投與 1%山苦瓜乙酸乙酯 萃取物於高脂飲食餵養之 ApoE 剔除雄鼠，可顯著的降低其新生內層與中層面積(1g/kgBW/day)，未顯著降低血液 TC、TG 及 NEFA 之結果相似(趙哲毅，2003)；
另一研究中，曾氏添加相同劑量 1%山苦瓜乙酸乙酯萃物於高脂飼料中，同樣無法 改善 6~10 週齡及 18 週齡 ApoE 剔除鼠血液 TC 與 TG 濃度(曾雅旻，2006，見表 4-1，實驗 1、2)。然而，曾氏於研究指出，山苦瓜乙酸乙酯萃物雖無法降低血脂，