Therapeutic concentrations of propofol
protects mouse macrophages from nitric
oxide-induced cell death and apoptosis
蔡世音
Chang H;Tsai SY;Chang Y;Chen TL;Chen RM
摘要
Abstract
Purpose To evaluate the potential effect of a clinically relevant concentration of propofol (PPF) on cell viability and nitric oxide-induced macrophage apoptosis.
Methods Mouse macrophages (cell line Raw 264.7) were cultured and incubated with a nitric oxide donor sodium nitroprusside (SNP), PPF, and a combination of PPF and SNP for one, six and 24 hr. Cell viability was determined by the colorimetric
3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Apoptotic cells were determined by analyzing the percentages of sub-G1 phase in macrophages. The amounts of nitric oxide were assayed.
Results The amounts of nitric oxide in macrophages were increased with time when incubated with SNP (P < 0.05). Simultaneously SNP caused cell death of macrophages in a concentration-and time-dependent manner (P < 0.05). PPFper se did not alter the amount of basal and SNP-provided nitric oxide in macrophages. A therapeutic concentration of PPF (30 μM) exhibited no cytotoxicity. After incubation with SNP for one and six hours, PPF could completely or partially block nitric oxide-induced cell death, respectively (P < 0.05).
Administration of SNP to macrophages resulted in a time-dependent pattern of increase of apoptotic cells (P < 0.05). Similar to the results of the cell viability analyses, PPF was able to protect macrophages from nitric oxide-induced apoptosis in one and six hour-treated groups (P < 0.05) but not in the 24 hr treated group.
Conclusion PPF at a therapeutic concentration, can protect mouse macrophagesin vitro from nitric oxide-induced cell apoptosis as well as cell death.
