藥科 2 心得報告 b303098155 陳胤淳
Scifinder 使用成果
Green tea polyphenol EGCG suppresses lung cancer cell growth through
upregulating miR-210 expression caused by stabilizing HIF-1α
By: Wang, Hong; Bian, Shengjie; Yang, Chung S.
(-)-Epigallocatechin-3-gallate (EGCG) has been reported to affect many cellular regulatory pathways. This study aims to det. whether EGCG could target microRNA (miRNA), one of the mechanisms for cells to achieve subtle change in multiple targets. We found that, in both human and mouse lung cancer cells in culture, EGCG specifically upregulated the expression of miR-210, a major miRNA regulated by HIF-1α. Furthermore, we found that overexpression of miR-210 led to reduced cell proliferation rate and anchorage-independent growth as well as reduced sensitivity to EGCG. On the mechanisms of miR-210 regulation by EGCG, we demonstrated that the regulation was mediated through the
hypoxia-response element in miR-210 promoter. Consistently, the upregulation of miR-210 was found to be correlated with the stabilized HIF-1α in lung cancer cell lines after EGCG treatment. This EGCG-induced stabilization of HIF-1α was further shown by the stabilization of HA-tagged HIF-1α but not the P402A/P564A-mutated HIF-1α by EGCG, suggesting that EGCG targets the
oxygen-dependent degrdn. (ODD) domain. Direct evidence was obtained by affinity binding assay showing that EGCG specifically binds HIF-1α with a Kd = 3.47 μM. This result suggests that EGCG binding interferes with the hydroxylation of key Pro residues in the ODD domain, preventing HIF-1α from the Pro hydroxylation-dependent ubiquitination and subsequent proteosome-mediated degrdn. In summary, our results demonstrated, for the first time, the elevation of miR-210 by EGCG in lung cancer cell lines and this is mediated by the stabilization of HIF-1α. This event contributes to the anticancer activity of EGCG.
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心得：
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Innovation 查詢使用結果
Health noodles containing green tea for preventing gastric cancer and
intestinal cancer
By: Chen, Xiaobo; Yu, Qinggui; Wang, Jianqiang
Assignee: Liyang Bailushan Ecological Agricultural Development Co., Ltd., Peop.
Rep. China
The title health noodles are manufd. from (by wt. parts): flour 95-97 and green tea
powder 3-5. The health noodles are rich in tea polyphenols, caffeine,
lipopolysaccharides, and theanine, and can prevent gastric cancer and intestinal cancer,
promote digestion, and prevent eye disease. The health noodles have the advantages
of high nutrition, good taste, no pigment, and beautiful color.
Patent Information
Patent No.
Kind
Date
Application No.
Date
CN 102178161
A
Sep 14, 2011
CN 2010-10558908
Nov 25, 2010
Priority Application
CN 2010-10558908
Nov 25, 2010
Indexing
Food and Feed Chemistry (Section 17-11)
Concepts
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Tea products
green tea leaves; health noodles contg. green tea for preventing gastric cancer and
intestinal cancer
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Eye disease
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Flours and Meals
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Health food
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Intestinal neoplasm
•
Pasta
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Triticum aestivum
•
Wheat
health noodles contg. green tea for preventing gastric cancer and intestinal
cancer
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Lipopolysaccharides
health noodles contg. green tea for preventing gastric cancer and intestinal
cancer
Food or feed use; Biological study; Uses
•
