新聞稿
臺北醫學大學 101 學年度碩士班暨碩士在職專班招生入學考試
分子生物學試題 本試題第 1 頁；共 2 頁 (如有缺頁或毀損，應立即請監試人員補發) 注 意 事 項 一、本試題共三大題，共計 100 分。 二、請將最適當的答案依題號作答於答案用卷本上。 三、試題答錯者不倒扣；題次號碼錯誤或不按順序或鉛筆作答，不予計分。 一、 選擇題：(每題 2 %，共 14%)1. Histones are _______ that are usually associated with _________.
acidic proteins; DNA acidic proteins; RNA basic proteins; DNA
basic proteins; RNA coenzymes derived from histidine; enzymes
2. Topoisomerases can:
change the linking number (Lk) of a DNA molecule. change the number of base pairs in a DNA molecule.
change the number of nucleotides in a DNA molecule. convert D isomers of nucleotides to L isomers. interconvert DNA and RNA.
3. At which phase of cell cycle, the cell contains 4n DNA ?
G1 S G2
M none of the above
4. Protein function can be regulated by sumoylation or phosphorylation. We call this situation as:
transcriptional control posttranscriptional control
translational control posttranslational control
none of the above
5. Protein expression is an important parameter monitoring gene’s function. One of the following techniques is NOT used in studying proteins:
ELISA Western blot analysis HPLC
FISH 2-dimentional electrophoresis
6. The restriction fragment length polymorphism (RFLP) is used to analyze
different DNA sequence different RNA sequence
different protein sequence none of the above
7. Which technique CAN BE used to “ knock down” gene expression ?
RNA interference Southern blot restriction mapping
marker exchange none of the above
二、 填充題：(每題 2 %，共 14%)
1. The protein terminates transcription for about half of all E. Coli mRNAs.
2. The assembly of general transcription factors to a eukaryotic promoter begins at the site of in a promoter.
3. In prokaryotes, can remove primer, replaces it with correct nucleotides, proofreads new strand, if errors found
exonuclease removes and repairs with correct nucleotides.
4. The is a protective structure at each end of an eukaryotic chromosome. Specifically, the tandemly repetitive DNA at the end of the chromosome's DNA molecule.
5. In prokaryotes, DNA replication begins at a unique site called: .
6. In the lac operon of E. coli, is the site which is downstream from the promoter but upstream from the
β-galactosidase gene.
新聞稿
臺北醫學大學 101 學年度碩士班暨碩士在職專班招生入學考試
分子生物學試題 本試題第 2 頁；共 2 頁
(如有缺頁或毀損，應立即請監試人員補發)
三、 問答題：(72%)
1. Please describe the following terms: (1) epigenetic modification (3%) (2) housekeeping gene (3%) (3) Okazaki fragment (3%)
(4) Shine-Dalgarno sequence (3%)
2. (1) Please describe the differences between reverse transcritase-PCR (RT-PCR) and standard PCR?(5%) (2) For what purpose would you use RT-PCR? (5%)
3. Please describe the mechanism of micro RNA in regulation of gene expression ? (10%) 4. Please describe the mechanism of RNA processing in Eukaryote cells.(10%)
5. What is the melting temperature (Tm)? What kind of factors will influence the Tm value? (10%)
6. Eukaryotic genomic DNA containing the Rb gene can be cloned into plasmid that replicates in E. coli, but the Rb protein
is not expressed from this plasmid in E. coli. Why? How can the expression of eukaryotic genes in bacteria be completed?
(10%)
