BHE Induces p53-dependent Trans-activation and Fas/CD95-dependent Apoptosis Pathway in HCT 116 human Colorectal Cancer Cells

| 會員名稱：張庭嘉 | 登出

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BHE Induces p53-dependent Trans-activation and Fas/CD95-dependent

Apoptosis Pathway in HCT 116 human Colorectal Cancer Cells

Ting-Chia Chang1*, Yu-Jen Chiu2, Kuang-Chi Lai3, Kuei-Li Lin4, Shu-Fen Peng5, Mann-Jen Hour6, Chao-Ying Lee6, Yi-An Jin1, Jing-Gung Chung5#, Wen-Wen Huang5#, Jai-Sing Yang1#

1

Department of Pharmacology, School of Medicine, China Medical University., 2Department of Medical Education, Far Eastern Memorial Hospital., 3Department of Surgery, China Medical University Beigang Hospital., 4Department of Radiation Oncology, Chi Mei Medical Center.,

5_{Department of Biological Science and Technology, China Medical University.,} 6_{School of}

Pharmacy, China Medical University.

In this study, we reported the cytotoxicity and action mechanism of BHE in HCT 116 human colorectal cancer cells. BHE showed growth inhibition, DNA damage and apoptosis in HCT 116 cells by using determination of MTT assay, morphologic changes, DAPI staining, comet assay and DNA gel electrophoresis. The results indicated that BHE induced the apoptotic cell death through extrinsic apoptotic pathway by the elevation of Fas/CD95, FADD and caspase-8 protein levels. Intracellular reactive oxygen species (ROS) generation was observed on BHE-stimulated HCT 116 cells. BHE induced early phosphorylation of p53Ser18 and histone H2AXSer139 through ataxia telangiectasia mutated (ATM) activation in response to ROS-induced DNA damage. The results of colorimetric assays revealed the activities of caspase-8 and caspase-3 were increased in BHE-treated cells. This is the first report on the activation of ROS-dependent ATM/p53 signaling as an important mechanism of BHE-induced cell death in HCT 116 human colorectal cancer cells.

BHE Induces p53-dependent Trans-activation and Fas/CD95-dependent

Apoptosis Pathway in HCT 116 human Colorectal Cancer Cells

1_{中國醫藥大學 醫學系 藥理學科,} 2_{亞東紀念醫院 醫學教育學科,} 3_{中國醫藥大學} 附設醫院 外科, 4奇美醫院 放射腫瘤科, 5中國醫藥大學 生物科技系, 6中國醫藥 大學 藥學系

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第27屆生醫年會--大會摘要預覽

2011/12/5

http://www.jacbs.org.tw/edit.aspx

張庭嘉1*, 邱宇任2, 賴光啟3, 林奎利4, 彭淑芬5, 侯曼貞6, 李昭瑩6, 金益安1, 鍾景光5#, 黃雯雯5#, 楊家欣1#

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第27屆生醫年會--大會摘要預覽

2011/12/5

http://www.jacbs.org.tw/edit.aspx