麻杏石甘湯對塵璊誘發氣喘天竺鼠之影響
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(2) 63. Dermatophagoides pteronyssinus. 1. 49.5. 9.7. 11.6. 4.2. 39.9. 9.2. 11.5. 4.7. 52.9. 7.4. 3.3. 1.3. 5.1. 28.3. 10.0. 29.2. 7.1. 19.4. 6.9. 13.5. 4.0. 25.4. 11.0. 15.6. 51.3. 8.0. 11.9. 3.5. 13.0. 5.8*. 32.6. 6.1. 36.7. 8.0. 10.8. 4.3. 28.0. 7.8. 24.5. 4.9. 48.0. 9.2. 4.3. 2.3. 15.2. 4.1. 23.8. 10.1. 21.0. 5.9. 41.9. 9.8. 16.1. 4.4. 22.3. 7.5. 23.1. 6.0. 38.6. 6.5. 13.3. 3.5. 21.7. 13.3. 18.1. 4.9. 29.6. 6.3 6.1 8.9. 51.2. 8.9. 3.2. 1.6. 19.9. 7.2. 48.9. 8.0. 17.8. 4.2. 13.5. 13.1. 4.4. 52.8. 14.5. 17.0. 7.7. 17.0. 47.9. 5.0. 5.0. 3.0. 16.3. 7.5. 30.9. 9.9. 21.2. 4.4. 45.6. 6.1. 7.0. 1.7. 26.3. 8.5. 18.7. 7.5. 53.3. 13.6. 11.1. 7.8. 16.9. 5.9. 46.0. 5.7. 4.3. 2.1. 12.7. 2.8. 37.1. 6.8. *p<0.05 p<0.01. p<0.05 p<0.01. Dermatophagoides pteronyssinus * p<0.01. p<0.01. [12-14] 350 30. 450. Dunkin-. 90. Hartley 30. DerP DerP. 30 100 g. 0 DerP. 7. 14 21. 4 mg/ml [15].
(3) 64. phosphate-buffered saline (PBS) ( 4. 4. 3. 3. 8). 7. [17]. 50. 5. 400xg. 15. 40. Eagle 1ml. minimum essential medium (MEM) (500xg , 15. ). 1g/ml. -20. [18]. [16]. Liu stain 5. 6. 1. 21. 2. 15. 4 mg/ml. 10 g/kg(1 g/ml) 4 mg/ml. DerP. 0.9%. 1. 6. 24. 48. 72. DerP 10. 5. 1. 2. 4. 6. 8. 12. 24. 48. 72. 1 24. 48. 10%. 6. 7. 72 5. 6 15. 2 0.9%. 10 ml/kg. 8. 1 5 m. 4 mg/ml. DerP. H.E. [19]. 10. F-test Duncan's Multiple-Range Test. 5. 6. (one way-. 100 g. DerP 0.9%. Range Test. 10. (Model GPBB-2. Duncan's Multiple-. ANOVA). Shizume 1. ). Medical Co.. 6. 8 24. (pressure/volume, P/V. ) P/V. 1. 2. 4. 6. (A). 12. 24. P/V. 5 48 (B). 5. 72. 1. B/A. 2. 2. 4. 6. 8. 12. 24. 48. 72 (. p<0.01) 1. 6. 24. 48. 72. 1 24. (p <0.01). (p<0.05) 48. 6. (p<0.05). (p <0.01). 72. (p<0.01) 5 ml. 1. (p<0.01). 6. 1,.
(4) 65. (p<0.05). 4 1. 6. 24. 48. 24. 72. [30-32] 1. (. 6. 1) Inoue 1. 6. TEI-7361 neutrophil elastase 24. 48. [33]. 72. neutrophil elastase 1. 6. Hutson 24. 48. 72. cromolyn sodium. [34]. disodium. cromoglycate [35]. DerP 24. 72. 10 5. 12 Dermatophagoides farinae. 12. DerP. [36] 1. 6. 12. Iijima [17]. [20,21] Hutson. Frew. (one second of force expiratory volume, [22]. FEV1). [37,. [23-26] 38] 72 [27] [28,29]. 24. 48.
(5) 66 bronchial reactivity. Clin Allergy 1977;7:503-13. 8.. Cartier A, Thomson NC, Frith PA, et al. Allergen-induced. increase. in. bronchial. responsiveness to histamine: relationship to the late asthmatic response and change in airway. (ephedrine). calibre. J Allergy Clin Immunol 1982;70:170-7.. (d-. pseudoephedrine). 2. -. 9.. Hargreave FE. Late-phase asthmatic responses and airway inflammation. J A l l e r g y C l i n Immunol 1987;83:525-7.. (amygdalin). 10. Barnes PJ, Chung KF, Page CP. Inflammatory mediators and Asthma. P h a r m a c o l. (glycyrrhizic acid). 18. —. Rev. 1988;40:49-72. 1980;22-4. 11.. 12. Ishii A, Ito K, Ino Y, et al. Experimental asthma in guinea pigs sensitized with mites (D.P). Int Arch Allergy Appl Immunol 1989;89:400-3.. (CaSO4. 13. Haida M, Okudaira H, Ogita T, et al. Allergens. 2H2O). of the house dust mite Dermatophagoides farinae-immunochemical studies of four. [39-42]. allergenic fractions. J Allergy Clin Immunol 1985;75:686-92. 14. Tovey ER, Chapman MD, Wells CW, et al. The distribution of dust mite allergen in the houses of patients with asthma. Am Rev Respir Dis 1981;124:630-5. 15. Hsiue TR, Lei HY, Hsieh AL, et al. Mite-induced. 2. allergic airway inflammation in guinea pigs. Int Arch Allergy Immunol 1997;112:295-302. 16. 1994;16:8-10 17. Iijima. H,. Ishii. M,. Bronchoalveolar 1.. Pepys J, Hutcheroft BJ. Bronchial provocation tests in etiologic diagnossis and analysis of asthma. Am Rev Respir Dis 1975;112:829-59.. 2.. O. Byrne PM, Dolovich J, Hargreave FE. Late. characterization of late asthmatic response in guinea pigs. Am Rev Respir Dis 1987;l36:922-9. 18. Hutson PA, Church MK, Clay TP, et al. EarIy. Hargreave FE, Dolovich J, Robertson DG, et al.. pig. Am Rev Respir Dis 1988;137:548-57. 19. Bai TR, Prasad FW. Abnormalities in airway smooth muscle in fatal asthma. Am Rev Respir Dis 1990;141:552-7. 20. Kay AB, Wadlaw AJ, Mobel R, et al. Leukocytes and the asthma process. In: Kay AB, ed. Allergy. The late asthmatic responses. Can Med Assoc J. and Inflammation. London: Academic Press,. 1974;110:415-21.. 1987:203-23.. Booij-Noord H, Orie NGM, de Vries K. Immediate and late bronchial obstructive. 21. Nagy. L,. Lee. TH,. Kay. AB.. Neutrophil. chemotactic activity in antigen-induced late. reactions to inhalation of house dust mite and. asthmatic reactions. N Engl J Med 1982;306:497-. provocative effects of disodium cromoglycate. 501.. and prednisolone. J Allergy Clin Immunol. 22. Sastre J, Banks DE, Lopez M, et al. Neutrophil. 1971;48:344-54.. chemotactic activity in toluene diisocyanate-. Robertson DG, Kerigan AT, Hargreave FE, et al.. induced asthma. J A l l e r g y C l i n I m m u n o l. Late asthmatic response induced by ragweed allergen. J Allergy Clin Immunol 1974;54:244-54. 7.. al.. and late-phase bronchoconstriction after. 1987;136:740-51.. 6.. et. allergen challenge of nonanesthetized guinea. asthmatic response. A m R e v R e s p i r D i s. 5.. K,. histologic. bronchial obstructive reaction to experimental 1972;2:43-61.. 4.. and. Booij-Noord H, de Vries K, Sluiter HJ, et al. Late inhalation of house dust extract. Clin Allergy. 3.. Yamauchi. lavage. 1990;85:567-72. 23. Metzger WJ, Mosely P, Nugent K, et al. Local. Cockcroft DW, Ruffic RE, Dolorich J, et al.. antigen challenge in bronchoalveolar lavage in. Allergen-induced increase in non-allergic. asthmatic patients. Chest I985;87(Suppl):155-6..
(6) 67 asthmatic responders. Am Rev Respir Dis. 24. Metzger WJ, Richerson HE, Worden K, et al. Bronchoalveolar lavage of allergic asthmatic patients following allergen bronchoprovocation.. 1993;147:76-81. 33. Inoue H, Kobayashi H, Shiba S. The Effect of. Chest 1986;89:483-7.. neutrophil elastase on airway constriction and. 25. De Monchy GR, Kauffman HF, Venge P, et al. Bronchoalveolar. eosinophilia. hyperresponsiveness. Abstract book. The. following. Second Asian Pacific Congress of Allergology. allergen-induced delayed asthmatic reactions. Am Rev Respir Dis 1985;11:373-6.. and Clinical Immunology 1995:l22. 34. Hutson PA, Holgate ST, Church MK. The effect. 26. Diaz P, Gonzales C, Galleguillos F, et al.. of cromolyn sodium and albuterol on early. Eosinophils and macrophages in bronchial. and late phase bronchoconstriction and airway. mucus and bronchoalveolar lavage during. leukocyte infiltration after allergen challenge. allergen-induced late-phase asthmatic reactions.. of nonanesthetized guinea pigs. Am Rev Respir. J Allergy Clin Immunol 1986;77:244-9.. Dis 1988;138:1157-63.. 27. Bousquet J, Chanez P, Lacoste JY, et al.. 35.. I. Eosinophilic inflammation in asthma. N Engl J Med 1990;323:1033-9.. 1983;8:43-5 36. Yu CK, Lee SC, Wang JY, et al. Early-type. 28. De Monchey JG, Kauffman HF, Venge P.. hypersensitivity associated airway inflamma-. Bronchoalveolar eosinophilia during allergen-. tion. induced late asthmatic reactions. Am Rev. Dermatophagoides Farinae in sensitized mice. J. Respir Dis 1985;131:373-6.. and. eosinophilia. induced. by. Immunol 1996;156:1923-30.. 29. Lam S, LeRiche J, Phillips D, et al. Cellular and. 37. Hutson PA, Church MK, Clay TP, et al. Early. protein changes in bronchial lavage fluid after. and late phase bronchoconstriction after. late asthmatic reactions in patients with red. allergen challenge of nonanesthetised guinea. cedar asthma. J A l l e r g y C l i n I m m u n o l. pig. (The association of disordered airway. 1987;80:44-50.. physiology to leukocyte infiltration). Am Rev. 30. Calhoun WJ, Jarjour NN, Gleich GJ, et al. Increased airway inflammation with segmental. Respir Dis 1988;137:548-57. 38. Frew AJ, moqbel R, Azzawi M. T lymphocytes. versus aerosol antigen challenge. Am Rev. and eosinophils in allergen-induced late-phase. Respir Dis 1993;147:1465-71.. asthmatic reactions in the guinea pig. Am Rev. 31. Liu MC, Hubbard WC, Proud D, et al. Immediate and late inflammatory responses to. Respir Dis 1990;141:407-13. 39.. ragweed antigen challenge of the peripheral airways in asthmatics: cellular mediator and permeability changes. Am Rev Respir Dis 1991;144:51-8. 32. Aalbers R, Kauffman HF, Vrugt B, et al. Bronchial lavage and bronchoalveolar lavage in allergen-induced. single. early. and. dual. 1993;15:48-9 40. 1991;13:36-7 41. 1989;10:134-5 42.. ,. ,. , 1998;30:40-1.
(7) 68. The Effect of Ma-Xing-Gan-Shi-Tang on Dermatophagoides pteronyssinusInduced Asthmatic Guinea Pig Shung-Te Kao, Jau-Dong Chang1, Tzuen-Ren Hisue3, Yih-Shyong Lai2, Jaung-Geng Lin1 Department of Chinese Medicine, China Medical College Hospital, 1Institute of Chinese Medical Sciences, and 2Department of Pathology, School of Medicine, China Medical College, Taichung; 3. Department of Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R.O.C.. Allergic asthma is one of the most common chronic pediatric diseases in Taiwan. According to an epidemiology study of asthma in schoolchildren in Taipei, the prevalence of asthma increased from 1.3% in 1974 to 10.8% in 1994. More then 90% of children with asthma have positive skin test responses to house dust mite (HDM), and Dermatophagoides pteronyssinus (DerP) was found to be the dominant species. MaXing-Gan-Shi-Tang (MXGST), a traditional Chinese medicine, has been used in treatment of the bronchial asthma for several centuries. To understand the mechanism of antiasthmatic property of MXGST, a guinea pig model of allergic asthma was used to investigate the effect of MXGST on DerP-induced early and late asthmatic responses and airway inflammation. The results showed administration of MXGST significantly inhibited the antigen induced immediate asthmatic responses and late asthmatic responses, and inhibited the increase in percent of neutrophil in bronchoalveolar lavage fluid at 1h, 6h after antigen challenge. Histopathologic examination showed MXGST suppressed the neutrophil infiltration into the airway at early phase. Otherwise, MXGST cannot suppress the eosinophil infiltration into the airway at late phase. In conclusion, M X G S T d o e s h a ve a n e ffe c t o n a s t h m a t r e a t m e n t , w h i c h c o u l d r e l i eve t h e bronchoconstriction and decrease neutrophil infiltration into airway. The precise mechanism of action of MXGST in asthma remains to be elucidated. ( Mid Taiwan J Med 1999. 4 62-8 ). Key words immediate asthmatic responses, inflammatory cell, late asthmatic responses, Ma-XingGan-Shi Tang. Received September 29, 1998.. Revised December 30, 1998.. Accepted January 5, 1999. Address reprint requests to. Shung-Te Kao, Department of. Chinese Medicine, China Medical College Hospital, No 2, YuhDer Road, Taichung, Taiwan, R.O.C..
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