試題 第 頁 1
高雄醫學大學 100 學年度研究所招生考試試卷
系所: 醫學檢驗生物技術學系碩士班
科目: 生物化學
一、解釋名詞： 20% (每題二分)
1. SNP 2. RNA interference 3. Entropy 4. plasmalogen 5. mannitol 6. Cellular senescence 7. proteomic 8. isozyme 9. uncoupling agent 10. cyclin二、配對題 20%
1)______ Glucose is removed from the blood and
phosphorylated by the ______ enzyme glucokinase. 2)______ Urea and other waste products are excreted by the
______.
3)______ The liver and muscle are metabolically linked by the ______.
4)______ The endocrine glands secrete ______.
5)______ Epinephrine binding to the ______ receptor causes an increase in [Ca2+].
6)______ The protein _____ was the first G protein–coupled receptor to be structurally analyzed at the atomic level.
7)______ Insulin binds to a receptor that possesses ______ activity.
8)______ A phosphatase activated by Ca2+, ______, is essential for T cell proliferation.
A) -adrenergic B) calcineurin C) Cori cycle D) pancreas E) leptin F) phosphatase G) liver H) hormones I) rhodopsin J) ketone bodies K) kidney L) tyrosine kinase
9)______ Following prolonged starvation, ______ are used as fuel by the brain. 10)______ A strain of genetically obese mice lack the protein ______.
三、選擇 10%
1. What is the overall net charge on the peptide lys-lys-ser-glu at pH 7.0? A) +2 B) +1 C) 0 D) -1 E) -2
試題 第 頁 2
2. The KM can be considered to be the same as the dissociation constant KS for E + S binding if:
A) this statement cannot be completed because KM can never approximate KS. B) ES E + P is fast compared to ES E + S.
C) the turnover number is very large. D) k2 k–1.
E) kcat/KM is near the diffusion-controlled limit.
3. The energy that is released by the hydrolysis of ATP by actin is used for:
A) actin filament assembly. B) actin filament disassembly. C) actin-myosin assembly. D) actin-myosin disassembly. E) muscle contraction.
4. Which of the following is (are) the result of insulin binding to its receptor? A) MAPK activation, which alters gene expression via Fos and Jun. B) PI3K activation leading to an increase in glucose transport.
C) Histone dephosphorylation resulting in decreased expression of glycogen synthase. D) A and B
E) A, B, and C
5. The nitrogen atom added to IMP to form AMP is from ____, and to form GMP is from ______. A) Asp, Phe B) Gln, Phe C) Asp, Gln D) Gly, Asp E) Gln, Asp
四、簡答題：20 % (每題 4 分)
1. Please draw the structure of the peptide Ala–His–Met. 2. What is the aetiology of β-thalassemia?
3. What kind of membrane proteins are cytochrome c and cytochrome oxidase? 4. What are the terminal electron acceptors in aerobic and anaerobic organisms?. 5. The first step in pyrimidine biosynthesis is the formation of the molecule ___?___
五、問答題 30% (每題 5 分)
1. What is meant by the statement “muscle carbohydrate metabolism serves only muscle”? 2. Explain why globin alone or heme alone is not effective as an oxygen carrier.
3. Why is it essential for survival that bacterial cells be surrounded by a cell wall? 4. What are the possible metabolic fate(s) of glucose-6-phosphate?
5. Describe the characteristics of glucokinase. How does it differ from other hexokinases? 6. What is the function of the GTP (or GDP) bound to tubulin?
