E D I T O R I A L
Response to a Letter to the Editor from Rodgers regarding
‘‘Overexpression of Myostatin2 in zebrafish reduces
the expression of dystrophin associated protein complex
(DAPC) which leads to muscle dystrophy’’
Aseervatham Anusha Amali
Æ Cliff Ji-Fan Lin Æ Yi-Hsuan Chen Æ
Wei-Lun Wang
Æ Hong-Yi Gong Æ Ravikumar Deepa Rekha Æ
Jenn-Khan Lu
Æ Thomas T. Chen Æ Jen-Leih Wu
Published online: 1 November 2008
Ó National Science Council Taipei 2008
We appreciate being made aware of earlier published work
on the cloning of zebrafish myostatin 2 (MSTN-2),
nomenclature of MSTN family. In the published version of
this article, we reported one zebrafish MSTN-2 cDNA
(GenBank accession number: AY614000), which is
dif-ferent from two published zebrafish MSTN-2 according to
comparison of nucleotide and deduced amino acid
sequences (Fig.
1b). However, two previous published
articles which identified zebrafish MSTN-2 cDNA
(Gen-Bank accession number: AY687474) [1] and gene
(GenBank accession number: AY693972) [2] was not
ref-erenced in this article. We apologize for neglecting
pertinent references. According to new nomenclature of
myostatin family [3], we construct new phylogenetic tree
including three zebrafish MSTN-2 to replace old Fig.
3.
References
1. Kerr T, Roalson EH, Rodgers BD (2005) Phylogenetic analysis of the myostatin gene sub-family and the differential expression of a novel member in zebrafish. Evol Dev 7(5):390–400
2. Biga PR, Roberts SB, Iliev DB, McCauley LA, Moon JS, Collodi P, Goetz FW (2005) The isolation, characterization, and expres-sion of a novel GDF11 gene and a second myostatin form in zebrafish, Danio rerio. Comp Biochem Physiol B Biochem Mol Biol 141(2):218–230
3. Rodgers BD, Roalson EH, Weber GM, Roberts SB, Goetz FW (2007) A proposed nomenclature consensus for the myostatin gene family. Am J Physiol Endocrinol Metab 292(2):E371–E372
A. A. Amali C. J.-F. Lin Y.-H. Chen W.-L. Wang H.-Y. Gong R. D. Rekha J.-L. Wu (&)
Laboratory of Marine Molecular Biology and Biotechnology, Institute of Zoology, Academia Sinica, Taipei, Taiwan e-mail: jlwu@gate.sinica.edu.tw
C. J.-F. Lin
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
W.-L. Wang
Institute of Fisheries Sciences, National Taiwan University, Taipei, Taiwan
J.-K. Lu
Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan
T. T. Chen
Department of Molecular and Cell Biology and Biotechnology Center, University of Connecticut, Storrs, CT, USA
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J Biomed Sci (2008) 15:843–845Fig. 1b Alignment of deduced amino acids of three zebrafish MSTN-2, including MSTN-2c (AY614000), MSTN-2b (AY693972) and MSTN-2a (AY687474). Variant amino acid residues in N-terminal of MSTN-2c and in unique domain within matured peptide of zebrafish 2c and MSTN-2b are indicated with asterisks and underlined, respectively
844 J Biomed Sci (2008) 15:843–845
Fig. 3 Phylogenetic tree of MSTNs. Phylogenetic and molecular evolutionary analyses were conducted using
Molecular Evolutionary Genetics Analysis MEGA 4 software (http://www. megasoftware.net/index.html). Tree construction and distance correction were followed by neighbor-joining and Poisson correction methods, respec-tively. Four GDF11 from mammalian and zebrafish were used as an out group. Sequen-ces, in addition to those in Fig. 2, used in construction of this phylogenetic tree are baboon MSTN (AF019619), goose MSTN (AY448009), turkey MSTN (AF019625), chicken MSTN (AY448007), dog MSTN (AY367768), pig MSTN (AY448008), horse MSTN (AB033541), goat MSTN (AY436347), sheep MSTN (AF019622), blue catfish MSTN-1 (AY540992), channel catfish MSTN-1 (AF396747), gilthead seabream MSTN-1 (AF258448), gilthead seabream MSTN-2 (AY046314), striped bass MSTN-1 (AF290910), white perch MSTN-1
(AF290911), white bass 1 (AF197194), shidrum 1 (AF316881), shidrum MSTN-2 (AY059386), human GDF-11 (NM_005811), mouse GDF-11 (NM_010272), rat GDF-11 (XM_343148) and zebrafish GDF-11 (AF411599) J Biomed Sci (2008) 15:843–845 845
