2011 食品營養細胞學實驗
Date Topics
02/15 Introduction
1. Outline of Cell Biology Experiments 2. Equipments of Cell Biology Experiments
3. Sterile Handling
02/22 Preparation of Medium
1. Content of Medium 2. Aseptic Technique
3. Sterile Filtration
03/01 Cell Culture and Subculture of Cells
03/08 Cell Growth Curve
03/15 Detection of Chromosome Content
03/22 Fluorescent Staining of Lysomes and Mitochondria 03/29 Assay of Cell Viability: MTT Test and Cell Freezing
04/05 春節放假一日
04/12 實驗期中考
04/19 Measurement of Intracellular calcium Levels and Cell Thawing
04/26 SDS Polyacrylamide Gel Electrophoresis (Sample and PAGE preparation) 05/03 SDS polyacrylamide Gel Electrophoresis
(Sample loading、stained and distain) 05/10 RNA Analysis (I): Isolation of RNA 05/17 RNA Analysis (II): Electrophoresis of RNA
05/24 Flow cytometery
05/31 實驗數據與分析(上台報告)
06/07 Check Out &實驗期末考
*灰色標示部分為該實驗課改成上午 AM 8:30 上課
