2011 食品營養細胞學實驗

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2011 食品營養細胞學實驗

Date Topics

02/15 Introduction

1. Outline of Cell Biology Experiments 2. Equipments of Cell Biology Experiments

3. Sterile Handling

02/22 Preparation of Medium

1. Content of Medium 2. Aseptic Technique

3. Sterile Filtration

03/01 Cell Culture and Subculture of Cells

03/08 Cell Growth Curve

03/15 Detection of Chromosome Content

03/22 Fluorescent Staining of Lysomes and Mitochondria 03/29 Assay of Cell Viability: MTT Test and Cell Freezing

04/05 春節放假一日

04/12 實驗期中考

04/19 Measurement of Intracellular calcium Levels and Cell Thawing

04/26 SDS Polyacrylamide Gel Electrophoresis (Sample and PAGE preparation) 05/03 SDS polyacrylamide Gel Electrophoresis

(Sample loading、stained and distain) 05/10 RNA Analysis (I): Isolation of RNA 05/17 RNA Analysis (II): Electrophoresis of RNA

05/24 Flow cytometery

05/31 實驗數據與分析(上台報告)

06/07 Check Out &實驗期末考

*灰色標示部分為該實驗課改成上午 AM 8:30 上課

數據

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參考文獻

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