題名:Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells.
作者:高淑慧
Au HK; Yeh TS; Kao SH; Shih CM; Hsieh RH; Tzeng CR 貢獻者:醫學檢驗暨生物技術學系
上傳時間:2009-10-02T08:57:57Z
摘要:OBJECTIVE: To evaluate the transcription and translation ability of mitochondria in terminally differentiated granulosa cells, these cells were incubated with ionic calcium. DESIGN: Prospective laboratory research.
SETTING: In vitro fertilization laboratory in a
university hospital. PATIENT(S): Granulosa cells were harvested from 50 female patients undergoing IVF. INTERVENTION(S): Analysis of mitochondrial gene
expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and of mitochondrial-encoded proteins by Western blot. MAIN OUTCOME
MEASURE(S): Comparison of the RNA expression levels of genes including cytochrome c oxidase subunit I (COX I), adenosine triphosphate synthase 6 (ATPase 6),
flavoprotein, and succinate-ubiquinone oxidoreductase, and protein levels of COX I and flavoprotein in
different calcium ion treatment groups. RESULT(S): There were dose-dependent increases in RNA expressions of the four genes analyzed from granulosa cells cultured in a serial concentration of calcium ions. This effect was abolished when cells were preincubated with the
extracellular calcium-chelating agent, Ethylene glycol-bis (2-aminoethylether)-N,N,N',N'-tetraacetic acid
(EGTA). The effect of ionic calcium on both the nuclear-and mitochondrial-encoded subunits also was determined. Expression levels of mitochondrial transcription factor A in RNA significantly increased in granulosa cells that were exposed for 24 and 48 hours to 0.5 and 1 microM A23187. CONCLUSION(S): The present study is the first
report to present calcium-dependent increases in the transcription and translation levels of both nuclear-encoded and mitochondrial-nuclear-encoded mitochondrial
respiratory enzyme subunits and also indicates that mitochondrial transcription factor A is involved in mitochondrial biogenesis.
