Integrated HDA-GT12 system offers efficient genetic information

Provide the Right Performance and Price to the Right Customers

HDA GT-12 ^TM System

High Performance Nucleic Acid Analyzer

Integrated HDA-GT12 system offers efficient genetic information

Applications for Applications for

DNA DNA Analysis Analysis

Instrument Instrument

Software Software Chemistry Chemistry

Technical Technical

Support Support

HDA-GT12™

Genetic Analyzer

Gel cartridge kits

• GCK-5000

• GCK-5000F

• GCK-10K

• GCK-RNA*

BioCalculator software

• Instrument Control

• Data Analysis System Maintenance

• Customer training

• Trouble shooting

Yesterday and Today

Classical method, which is labor-intensive, highly dependent on operator technique, time-consuming, unreliable, slow, and often a subjective process that can take days. Interpreting samples is costly since a high level of skill is required

Today and Tomorrow

6 2 2 527

9 1 5

2 6 3 4

6 7 1 2 3

7 6 1 4 7

9 9 0

1 10

1 9 0 2 1 7

1 8 0 1 6 0 1 6 0

2 0 1 2 3 8

2 4 2 3 09

6 2 2 527

9 1 5

2 6 3 4

6 7 1 2 3

7 6 1 4 7

9 9 0

1 10

1 9 0 2 1 7

1 8 0 1 6 0 1 6 0

2 0 1 2 3 8

2 4 2 3 09

Technology of Capillary Electrophoresis

eGene’s Designed Platform

Patents Awarded Technology

eGene’s Designed Platform

Capillary Electrophoresis Patents Awarded Technology

HDA GT-12 ^TM System

‰ Capillary Electrophoresis

‰ Light-Induced Florescence Detection

‰ Polymer gel and EtBr intercalating dye

-1 0 1 2 3 4 5

3.0 4.0 5.0 6.0 7.0

Tim e (m inute )

RFU

Slab gel electrophoresis

HDA System electrophoresis

Advantages of HDA-GT12 System for DNA analysis

‰ Automated system (auto sample loading)

‰ Fast separation (around 10 min separation time)

‰ High resolution (2-5 bp within 500 bp)

‰ High sensitivity (Detect ng – pg/μl)

4 base pair resolution of the HDA system in 10 minutes as compared to a 8% acrylamide gel which runs for hours.

553 713 500 427

417 311 413

249 200 151 140 118 100 82 66

24 40 42 48 726

Functional Features (1)

‰ Provide high-resolution electrophoresis

• Achieve 2-5 bp resolution in 10 minutes

Functional Features (2)

‰ Provides high-detection sensitivity

• Achieve pico-gram level for one shot

• Achieve 0.1 ng/μl for undiluted PCR amplified DNA

Series dilution of PCR products (indicated by arrows) amplified in 50ml reaction.

The original 30ng/μl of PCR DNA was diluted up to 12800x by water and the results showed that HDA system can detect the DNA concentration as low as 2.3pg/μl.

bp

PCR Dilution Factor

1x 10x 50x 100x 200x 400x 800x 1600x 3200x 6400x 12800x

80

102

174

257

267

289

434

458

587

DNA samples in a 96 well-plate were analyzed in 40 minutes. An individual window was blown up to show high speed resolution of DNA ranged 15bp to 5kb and data analysis was provided by

BioCalculator ^TM Software.

Functional Features (3)

‰ Provides high-throughput DNA screening

• Achieve 96 samples in one step

Special display modes of

HDA GT-12 ^TM System

Design for DNA Electrophoresis Users

‰ Collect digital data

‰ Present in gel view format

Separation in 10 minutes Separation in 1.5 hours

Design for DNA Electrophoresis Users

(Continued)

‰ Multiple data comparison

• 36 data in one row

‰ Flexible data analysis (arrange, zoom, contract )

Design for Genotyping Users

‰ Present in electropherogram

‰ Data calculation

Design for Genotyping User

(Continued)

Multiple data comparison in electropherogram

‰ Automatic data collection/analysis

• For 12 samples in 5-10 minutes

• For 96 samples in 0.5-1.5 hours

Easy-To-Use Operation

‰ Easy As 1-2-3 for PCR product screening

• Insert Cartridge

• Place PCR amplified DNA into system

• Select method to run

Usages of HDA GT

Usages of HDA GT - - 12 12 ^{TM TM} System System

D D iagnostic Applications iagnostic Applications

Screening for Pathogens Detection

Detection of Hepatitis B Virus (HBV) DNA

Sample 1 Sample 2

4 34 4 58

80 102 80 102 80 102 1 74 2 57 2 67 2 57 2 67 2 89 4 58 5 87

b p U p per m a rke r

L ow er m a rke r

18 6b p

T o tal an alysis tim e: 10 m inu tes 4 34

4 58

80 102 80 102 80 102 80 102 80 102 80 102 80 102 1 74 2 57 2 67 2 57 2 67 2 57 2 67 2 57 2 67 2 89 4 58 5 87

b p U p per m a rke r

L ow er m a rke r

18 6b p

T o tal an alysis tim e: 10 m inu tes

HBV DNA Marker

Detection of Hepatitis B Virus (HBV) DNA

Sample 1 Sample 2

4 34 4 58

80 102 80 102 80 102 1 74 2 57 2 67 2 57 2 67 2 89 4 58 5 87

b p U p per m a rke r

L ow er m a rke r

18 6b p

T o tal an alysis tim e: 10 m inu tes 4 34

4 58

80 102 80 102 80 102 80 102 80 102 80 102 80 102 1 74 2 57 2 67 2 57 2 67 2 57 2 67 2 57 2 67 2 89 4 58 5 87

b p U p per m a rke r

L ow er m a rke r

18 6b p

T o tal an alysis tim e: 10 m inu tes

HBV DNA Marker

Detection of Mycobacterium tuberculosis DNA

M. tuberculosis Sample 2

DNA Marker

434 458

80 102 80 102 80 102 174 257 267 257 267 289 458 587

bp Upper marker

Lower marker

Total analysis time: 10 minutes

219bp 434

458

80 102 80 102 80 102 80 102 174 257 267 257 267 289 458 587

bp Upper marker

Lower marker

Total analysis time: 10 minutes

219bp

Sample 1

Sample 3 Sample 4

Detection of Mycobacterium tuberculosis DNA

M. tuberculosis Sample 2

DNA Marker

434 458

80 102 80 102 80 102 174 257 267 257 267 289 458 587

bp Upper marker

Lower marker

Total analysis time: 10 minutes

219bp 434

458

80 102 80 102 80 102 80 102 174 257 267 257 267 289 458 587

bp Upper marker

Lower marker

Total analysis time: 10 minutes

219bp

Sample 1

Sample 3 Sample 4

Typing of Human Papillomaviruses (HPV) DNA

Control

4 5 0 b p

2 5 0 b p 4 3 4

4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

b p

T o t a l a n a l y s i s t i m e : 1 0 m i n u te s 4 5 0 b p

2 5 0 b p 4 3 4

4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

b p

4 5 0 b p

2 5 0 b p 4 3 4

4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

b p

4 3 4 4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

b p

T o t a l a n a l y s i s t i m e : 1 0 m i n u te s

Oncogenic H PV S am pl e

DN A M ar ke r

Typing of Human Papillomaviruses (HPV) DNA

Control

4 5 0 b p

2 5 0 b p 4 3 4

4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

b p

T o t a l a n a l y s i s t i m e : 1 0 m i n u te s 4 5 0 b p

2 5 0 b p 4 3 4

4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

b p

4 5 0 b p

2 5 0 b p 4 3 4

4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

b p

4 3 4 4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

b p

T o t a l a n a l y s i s t i m e : 1 0 m i n u te s

Oncogenic H PV S am pl e

DN A M ar ke r

Identification of Plasmodium species (Malaria detection)

4 3 4 4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

bp U p p e r m a r k e r

L o w e r m a r k e r

269bp 436bp 395bp 499bp

T o ta l an a ly sis tim e: 1 0 m in u te s

4 3 4 4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

bp U p p e r m a r k e r

L o w e r m a r k e r

269bp 436bp 395bp 499bp

T o ta l an a ly sis tim e: 1 0 m in u te s

alariae P. O

vale

P. Falciparum P. Vivax

Identification of Plasmodium species (Malaria detection)

4 3 4 4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

bp U p p e r m a r k e r

L o w e r m a r k e r

269bp 436bp 395bp 499bp

T o ta l an a ly sis tim e: 1 0 m in u te s

4 3 4 4 5 8 5 8 7

8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 8 0 1 0 2 1 7 4 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 2 5 7 2 6 7 2 8 9 4 3 4 4 5 8 5 8 7

bp U p p e r m a r k e r

L o w e r m a r k e r

269bp 436bp 395bp 499bp

T o ta l an a ly sis tim e: 1 0 m in u te s

alariae P. O

vale

P. Falciparum P. Vivax

Screening in Genetic Diseases

305bp 305bp

311bp

305bp

311bp

Total automatic analysis time: 10 minutes

305bp 305bp

311bp

305bp

311bp

311bp 305bp 305bp

311bp

305bp

311bp

Total automatic analysis time: 10 minutes

Molecular Diagnosis of Bardet-Biedl Syndrome (Short Tandem Report Polymorphism)

Normal Hetero Homo Marker

Molecular Diagnosis of Hereditary

Inclusion Body Myopathy (H.I.B.M)

Screening in Genetic Diseases

Demonstration of STR/Microsatellite Analysis

Molecular Diagnosis of Down Syndrome

App Dyrk1a Acta1

Anal Biochem. (2005) 340:213-219

Quantitative Assay of Deletion or Duplication Genotype by Capillary Electrophoresis System:

Application in Prader–Willi Syndrome and Duchenne Muscular Dystrophy

Prader–Willi Syndrome

Duchenne Muscular Dystrophy

Clin. Chem. (2006) 10: 1373-1378

HLA-A “02” SSP Subtyping

(48 genetics markers in one patient)

16

1 7 0 b p 2 3 0 b p 1 7 5 b p 8 0 b p 1 2 0 b p

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

M 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32

M 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

2 2 8 b p

Detection of Muscular Dystrophy genotypes Detection of Muscular Dystrophy genotypes

via Universal Condition Direct Sequencing

via Universal Condition Direct Sequencing

Multiplex PCR Analysis

Multiplex PCR Analysis

Alignment Marker

10plex PCR

Calibration Marker

1 3 5 3

7 2 1 9 4 1 0 7 8

8 7 2

3 1 0 6 0 3

2 8 1

2 7 1

1 8 8

1 1 8 2 5 3 4 1 9

3 1 4 4 9 5 5 9 9

2 3 4 7 7 6 8 2 3 8 7 7

6 5 3

DNA Size Marker

10plex PCR

The 10plex panel detection of antibiotic resistance genes in methicillin-resistant Staphylococcus aureus (MRSA)

Alignment

Marker

16plex PCR

Calibration Marker

Calibration Marker

5 8 7

8 0 1 0 2 4 5 8

4 3 4

2 6 7 2 9 8

2 5 7

1 7 4 5 9 9

1 8 5 1 9 4 2 0 8 2 3 1 2 5 7 2 7 0 2 9 8 3 2 2 3 4 7 3 6 8 3 9 9 4 3 6 4 8 4 5 1 5 5 5 0

P ri m e r

DNA Size Marker

Alignment Marker

Alignment Marker Primer

Analysis of 16plex PCR DNA in Cystic Fibrosis Gene

for Mutation Detection

Genetic Analysis in Food Products

Genetic Analysis in Food Products

Genetic Analysis in Food Products

Analysis of Vertebrate Species Specific PCR amplified DNA in Meat

80 102 174 257 267 289 434 458 587

Goat Chicken Cow Sheep Pig Horse

Negative Control DNA

Marker

157 bp

439 bp 398 bp

329 bp 274 bp

227 bp

Total automatic analysis time: 10 minutes bp

Analysis of Vertebrate Species Specific PCR amplified DNA in Meat

80 102 174 257 267 289 434 458 587

80 102 80 102 174 257 267 289 257 267 289 434 458 434 458 587

Goat Chicken Cow Sheep Pig Horse

Negative Control DNA

Marker

157 bp

439 bp 398 bp

329 bp 274 bp

227 bp

Total automatic analysis time: 10 minutes bp

Detection of Genetic Modified Organism (GMO) Specific PCR Amplified DNA in Food Materials

80 102 174 257 267 289 434 458 587

DNA Marker

Maize GMO

Soya Control Maize

Control

Soya GMO

bp

225 bp

190 bp

118 bp

190 bp

Total automatic analysis time: 10 minutes

Detection of Genetic Modified Organism (GMO) Specific PCR Amplified DNA in Food Materials

80 102 80 102 174 257 267 289 434 458 587

DNA Marker

Maize GMO

Soya Control Maize

Control

Soya GMO

bp

225 bp

190 bp

118 bp

190 bp

Total automatic analysis time: 10 minutes

Multicapillary

Multicapillary electrophoresis analysis electrophoresis analysis of single

of single - - nucleotide sequence variations nucleotide sequence variations

Multicapillary electrophoresis analysis of single-nucleotide sequence variations in the deoxycytidine kinase gene

Clinical Chemistry (2006) 52:1756–1762

Clinical Chemistry (2006) 52:1756–1762

T T argeting argeting Induced I nduced L L ocal L ocal Lesion esion in in G G enome enome

5’ 3’

3’ 5’

5’ 3’

3’ 5’

5’ 3’

3’ 5’ 3’ 5’

3’ 5’

PCR amplify

Denature & Re-annealing

5’ 3’

5’ 3’

5’ 3’ 3’

5’

Nuclease CEL1 cuts at mis-match

PCR Amplification of Target Gene from Pooled Genomic DNA

Wild type gene

Mutant gene

Japan Rice Genetics Newsletter 2005 Vol. 22, 89

A modified TILLING system for rice mutant screening

Laboratory

Laboratory Applications Applications

Plasmid Cloning

(Large DNA fragments analysis )

500

1500

1000 4500

4000 3000 3500

2500

8500 2000

6000

5500 5000

Oligonucleotides Separation

18-mer 21-mer

19-mer

20-mer

21-mer

22-mer

23-mer

24-mer

RNA Fragments Size Analysis

Total automatic analysis time: 10 minutes

281

2604 1908 1383 955 623

3638 4981

28 S

18 S

50 25 ^{12.5 6.125}

RNA concentration in solution (ng/μl)

Total automatic analysis time: 10 minutes

Total RNA Quality Analysis

Multiple Applications in One System

AFLP

PCR DNA Screening

STR/microsatellite Analysis Oligo Analysis RNA Analysis

Plasmid cloning

eGene’s Cartridge Products HDA-GT12™ DNA Analyzer

GCK-5000F GCK-10K

8-channel 4-channel

Choice based on the Sample capacity Choice based

on type of Application ANAL Y S IS

12-channel

RNAQC*

GCK-5000

Yellow system service key Green data analysis key Blue system operation key

Distributors/

Application Scientists/ Collaborators Field Service Engineers

Instrument Control, Data Analysis, Method Development,

Maintenances and troubleshooting

Users Data Analysis

Users Instrument Control, Data Analysis

BioCalculator ^TM Software

Software keys Software CD

Instrument Control Data Analysis

= +

Choice of data Output

• Full plate and single data report by BioCalculator

• Gel view and electrophenogram image in JPEG format

• Real time and analysis data in Microsoft Excel format.

• Single data report in HTML format.

‰ Right Performance ‰ 9 Right Resolution 9 9 Right Sensitivity 9 Right Throughput

‰ Acceptable Cost

HDA-GT12 System Provides Total Solution

for Current and Future Genotyping Works

17841 Fitch

Irvine, CA 92614 USA Tel:(949)250-8686 Fax:(949)250-8833 www.egeneinc.com

Contact Information

台灣總代理

瑞林生物科技股份有限公司

台北市松德路72號7樓

電話：02-27230886 傳真：02-27230876

瑞林網址http://www.prismabiotech.com.tw

Resolution of GCK-5000F

Resolution of GCK-5000